https://m2.mtmt.hu/ hun 2026-05-31 13:58 JournalArticle 30378596 VALIDATED true Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary Nanobiosensorics Laboratory MTA-EK-MFA, Budapest, Hungary MTA-SE Immune-Proteogenomics Extracellular Vesicle Research Group, Budapest, Hungary Discovery Biology, Discovery Sciences, IMED Biotech Unit, AstraZeneca, Alderley Park, United Kingdom Cited By :63 Export Date: 10 October 2024 Correspondence Address: Buzás, E.I.; Department of Genetics, Hungary; email: buzas.edit@med.semmelweis-univ.hu 0 false 2026-05-19T00:29:25.017+0000 2024-10-07T09:42:50.146+0000 2019-01-08T08:36:46.226+0000 true 10026962 Visnovitz Tamás /api/author/10026962 Author true 10026962 SUSPECT 2026-03-20T08:00:38.497+0000 2026-03-20T08:00:38.497+0000 2024-10-07T09:42:50.518+0000 true 10014559 Bolgár Andrea /api/admin/10014559 Admin true 10014559 Admin 10067876 /api/admin/10067876 Ladányi Gusztáv true true Admin 10014568 /api/admin/10014568 Vándor Andrea true 10014568 true 2024-10-17T16:44:08.551+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Visnovitz, T An improved 96 well plate format lipid quantification assay for standardization of experiments with extracellular vesicles true true /api/journal/10029166 REVIEWED true false 10029166 false 10029166 true 2001-3078 Journal FOREIGN 8 1 1565263 1565263 12 2019 The field of extracellular vesicles is an exponentially growing segment of biomedical sciences. However, the problems of normalisation and quantification of extracellular vesicle samples have not been completely solved yet. Currently, extracellular vesicle samples are standardized on the basis of their protein content sometimes combined with determination of the particle number. However, even this combined approach may result in inaccuracy and overestimation of the extracellular vesicle concentration. Lipid bilayers are indispensable components of extracellular vesicles. Therefore, a lipidbased quantification, in combination with the determination of particle count and/or protein content, appears to be a straightforward and logical approach for the extracellular vesicle field. In this study we set the goal to improve the previously reported sulfo-phospho-vanillin total lipid assay. We introduced an aqueous phase liposome standard (DOPC) to replace the purified lipid standards in organic solvents (used commonly in previous studies). Furthermore, we optimized the concentration of the vanillin reagent in the assay. We found that elimination of organic solvents from the reaction mixture could abolish the background colour that interfered with the assay. Comparison of the optimised assay with a commercial lipid kit (based on the original sulfo-phospho-vanillin lipid assay) showed an increase of sensitivity by approximately one order of magnitude. Thus, here we report a quick, reliable and sensitive test that may fill an existing gap in extracellular vesicle standardisation. When using the optimised total lipid assay reported here, extracellular vesicle lipid measurements can be as easy as measuring proteins with a simple BCA test. true false 2019 true true true false true false GOLD 2026-05-19 true https://doi.org/10.1080/20013078.2019.1565263 97 0 90 68 24 91 89 90 85 93 88 71 66 74 23 0 97 74 90 68 0 7 7 Folyóiratcikk Journal Article 24 85 Könyvrészlet Chapter in Book 25 3 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 1 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 1 Disszertáció Thesis 28 6 Egyéb Miscellaneous 29 1 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2019 0 4 1 4 1 2020 0 8 4 8 4 2021 0 19 17 19 17 2022 0 8 6 8 6 2023 0 22 17 22 17 2024 0 15 12 15 12 2025 0 15 13 15 13 2026 0 6 4 6 4 D1 false 10010387,10026962,10040711,183 false false false 2019-01-29T12:22:21.785+0000 2019-01-08T11:17:48.896+0000 Anatómiai, Sejt- és Fejlődésbiológiai Tanszék (ELTE / TTK / Bio_I); Anyag- és Környezetkémiai Intézet (TTK); Biológiai Nanokémiai Kutatócsoport (HRN TTK / AKI); Fotonika Laboratórium (HUN-REN EK / MFA); Genetikai, Sejt- és Immunbiológiai Intézet (SE / AOK / I); MTA-SE Immun-proteogenomikai Extracelluláris Ve... (SE / AOK / I / GSII); Műszaki Fizikai és Anyagtudományi Intézet (HUN-REN EK) 12 71 7 12 0 true 10014568 10067876 Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 85393518 /api/authorship/85393518 1 0.083333336 true false Author 10026962 /api/author/10026962 Visnovitz Tamás true 10026962 true Visnovitz T true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 84942160 /api/authorship/84942160 2 0.083333336 false false Author 10052584 /api/author/10052584 Osteikoetxea Xabier true 10052584 true Osteikoetxea X true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 85393519 /api/authorship/85393519 3 0.083333336 false false Author 10043376 /api/author/10043376 Sódar Barbara true 10043376 true W. Sódar B true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 85393520 /api/authorship/85393520 4 0.083333336 false false Author 10010387 /api/author/10010387 Mihály Judith true 10010387 true Mihály J true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 85393521 /api/authorship/85393521 5 0.083333336 false false Author 10020615 /api/author/10020615 Lőrincz Péter true 10020615 true Lőrincz P true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 85393522 /api/authorship/85393522 6 0.083333336 false false Author 10040711 /api/author/10040711 Visnovitzné Dr Vukman Krisztina true 10040711 true V. 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true false true PersonAuthorship 85393526 /api/authorship/85393526 11 0.083333336 false false Author 10014807 /api/author/10014807 Varga Zoltán true 10014807 true Varga Z true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PersonAuthorship 85393527 /api/authorship/85393527 12 0.083333336 false true true Author 10015596 /api/author/10015596 Buzás Edit Irén true 10015596 true Buzás E I true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false true PublicationIdentifier 15163032 /api/publicationidentifier/15163032 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.1080/20013078.2019.1565263 https://doi.org/10.1080/20013078.2019.1565263 true true PublicationIdentifier 15213832 /api/publicationidentifier/15213832 SwordSource 36 /api/publicationsource/36 PublicationSourceType 10007 /api/publicationsourcetype/10007 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/api/publication/30378596 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10026962"><a href="/gui2/?type=authors&mode=browse&sel=10026962" target="_blank">Visnovitz T (<span class="authorship-author-name">Visnovitz Tamás</span> <span class="authorAux-mtmt"> Biológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Genetikai, Sejt- és Immunbiológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10052584"><a href="/gui2/?type=authors&mode=browse&sel=10052584" target="_blank">Osteikoetxea X (<span class="authorship-author-name">Osteikoetxea Xabier</span> <span class="authorAux-mtmt"> immunológia, genetika</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Genetikai, Sejt- és Immunbiológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10043376"><a href="/gui2/?type=authors&mode=browse&sel=10043376" target="_blank">W. Sódar B (<span class="authorship-author-name">Sódar Barbara</span> <span class="authorAux-mtmt"> immunológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Genetikai, Sejt- és Immunbiológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10010387"><a href="/gui2/?type=authors&mode=browse&sel=10010387" target="_blank">Mihály J (<span class="authorship-author-name">Mihály Judith</span> <span class="authorAux-mtmt"> IR és Raman spektroszkópia, biofizika</span>) </a> </span> <span class="author-affil"><span title="MTA Természettudományi Kutatóközpont">MTA TTK</span>/<span title="Anyag- és Környezetkémiai Intézet">AKI</span>/Biológiai Nanokémiai Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10020615"><a href="/gui2/?type=authors&mode=browse&sel=10020615" target="_blank">Lőrincz P (<span class="authorship-author-name">Lőrincz Péter</span> <span class="authorAux-mtmt"> Sejtbiológia</span>) </a> </span> <span class="author-affil"><span title="Eötvös Loránd Tudományegyetem">ELTE</span>/<span title="Természettudományi Kar">TTK</span>/<span title="Biológiai Intézet">Bio_I</span>/Anatómiai, Sejt- és Fejlődésbiológiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10040711"><a href="/gui2/?type=authors&mode=browse&sel=10040711" target="_blank">V. Vukman K (<span class="authorship-author-name">Visnovitzné Dr Vukman Krisztina</span> <span class="authorAux-mtmt"> biológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Genetikai, Sejt- és Immunbiológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10063768"><a href="/gui2/?type=authors&mode=browse&sel=10063768" target="_blank">Tóth E Á (<span class="authorship-author-name">Tóth Eszter Ágnes</span> <span class="authorAux-mtmt"> immunológia - extracelluláris vezikulák</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Genetikai, Sejt- és Immunbiológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10071123"><a href="/gui2/?type=authors&mode=browse&sel=10071123" target="_blank">Koncz A (<span class="authorship-author-name">Koncz Anna</span> <span class="authorAux-mtmt"> Biológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Genetikai, Sejt- és Immunbiológiai Intézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="183"><a href="/gui2/?type=authors&mode=browse&sel=183" target="_blank">Székács I (<span class="authorship-author-name">Székács Inna</span> <span class="authorAux-mtmt"> nanobioszenzorika</span>) </a> </span> <span class="author-affil"><span title="Energiatudományi Kutatóközpont">EK</span>/Műszaki Fizikai és Anyagtudományi Intézet; <span title="Energiatudományi Kutatóközpont">EK</span>/<span title="Műszaki Fizikai és Anyagtudományi Intézet">MFA</span>/Fotonika Laboratórium</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10002473"><a href="/gui2/?type=authors&mode=browse&sel=10002473" target="_blank">Horváth R (<span class="authorship-author-name">Horváth Róbert</span> <span class="authorAux-mtmt"> Bioszenzorok</span>) </a> </span> <span class="author-affil"><span title="Energiatudományi Kutatóközpont">EK</span>/<span title="Műszaki Fizikai és Anyagtudományi Intézet">MFA</span>/Fotonika Laboratórium</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10014807"><a href="/gui2/?type=authors&mode=browse&sel=10014807" target="_blank">Varga Z (<span class="authorship-author-name">Varga Zoltán</span> <span class="authorAux-mtmt"> biofizika, kolloidkémia</span>) </a> </span> <span class="author-affil"><span title="Természettudományi Kutatóközpont">TTK</span>/Anyag- és Környezetkémiai Intézet; <span title="MTA Természettudományi Kutatóközpont">MTA TTK</span>/<span title="Anyag- és Környezetkémiai Intézet">AKI</span>/Biológiai Nanokémiai Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10015596"><a href="/gui2/?type=authors&mode=browse&sel=10015596" target="_blank">Buzás E I ✉ (<span class="authorship-author-name">Buzás Edit Irén</span> <span class="authorAux-mtmt"> Immunbiológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/Genetikai, Sejt- és Immunbiológiai Intézet; <span title="Semmelweis Egyetem">SE</span>/<span title="Általános Orvostudományi Kar">AOK</span>/<span title="Intézet">I</span>/<span title="Genetikai, Sejt- és Immunbiológiai Intézet">GSII</span>/MTA-SE Immun-proteogenomikai Extracelluláris Vezikula Kutatócsoport</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;30378596" target="_blank">An improved 96 well plate format lipid quantification assay for standardization of experiments with extracellular vesicles</a></div> <div> <span class="journal-title">JOURNAL OF EXTRACELLULAR VESICLES</span> <span class="journal-issn">( <a target="_blank" href="https://portal.issn.org/resource/ISSN/2001-3078">2001-3078</a>)</span>: <span class="journal-volume">8</span> <span class="journal-issue">1</span> <span class="page"> Paper 1565263. 12 p. </span> <span class="year">(2019)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1080/20013078.2019.1565263" target="_blank" href="https://doi.org/10.1080/20013078.2019.1565263"> DOI </a> </span> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="91469" target="_blank" href="http://real.mtak.hu/91469"> REAL </a> </span> <span class="id identifier oa_GREEN" title=" Green "> <a style="color:blue" title="6780" target="_blank" href="https://repo.lib.semmelweis.hu/handle/123456789/6780"> SE Repozitórium </a> </span> <span class="id identifier oa_GREEN" title=" Green "> <a style="color:black" title="67455" target="_blank" href="https://edit.elte.hu/xmlui/handle/10831/67455"> EDIT </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="MEDLINE:30728922" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/MEDLINE:30728922"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="85060808259" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85060808259"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="30728922" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=30728922&dopt=Abstract"> PubMed </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="8186285396329745346" target="_blank" href="http://scholar.Google.com/scholar?hl=en&lr=&cluster=8186285396329745346"> Google scholar </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Cell Biology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Histology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 97</span> | Független: 74 | Függő: 23 | Nem jelölt: 0 | WoS jelölt: 90 | Scopus jelölt:&nbsp;85 | WoS/Scopus jelölt:&nbsp;93 | DOI jelölt:&nbsp;89 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;30378596&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 7 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 30378596</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> Forrás Idéző Duplumgyanú | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="lastModified">Utolsó módosítás: 2024.10.07. 11:42 Bolgár Andrea (MFA admin 4) </div> <div class="lockedBy">Központi kezelésű 2024.10.17. 18:44 Ladányi Gusztáv (MTMT API user, admin) Vándor Andrea (VA, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary ...</pre> </div></div>