The leaves of Syringa oblata Lindl., Oleaceae, had been extensively used as a folk
medicine to treat various infections, heal inflammations, icteric hepatitis and acute
mastitis. The study was designed to evaluate the hepatoprotective activity of S. oblata
leaves ethanol extract against CCl4-induced hepatotoxicity in primary hepatocytes
and mice with the indicator of glutathione S-transferase alpha 1. The hepatoprotective
effects of S. oblata leaves ethanol extract were evaluated on the basis of liver histopathology
and biochemical parameters as well as hepatic oxidative stress markers. The results
showed that CCl4 negatively modulated biochemical parameters and liver antioxidant
activities. However, the use of S. oblata leaves ethanol extract restored altered-serum
biochemical parameters and liver antioxidant activities in a dose-dependent manner.
Importantly, the trends in S-transferase alpha 1 were similar to alanine aminotransferase
and aspartate aminotransferase level, and S-transferase alpha 1 was suggested to be
a marker for the evaluation of hepatoprotective activity of S. oblata leaves ethanol
extract. Histopathological examination showed that CCl4 causes significant hepatic
injury relative to control group. The above findings suggested that S. oblata leaves
ethanol extract has hepatoprotective effects against CCl4-induced hepatic injury and
S-transferase alpha 1 may be an indicator to evaluate the protective effects of S.
oblata leaves ethanol extract. (C) 2018 Sociedade Brasileira de Farmacognosia. Published
by Elsevier Editora Ltda.