We introduce a method that combines two-photon polymerization (TPP) and surface functionalization
to enable the indirect optical manipulation of live cells. TPP-made 3D microstructures
were coated specifically with a multilayer of the protein streptavidin and non-specifically
with IgG antibody using polyethylene glycol diamine as a linker molecule. Protein
density on their surfaces was quantified for various coating methods. The streptavidin-coated
structures were shown to attach to biotinated cells reproducibly. We performed basic
indirect optical micromanipulation tasks with attached structure-cell couples using
complex structures and a multi-focus optical trap. The use of such extended manipulators
for indirect optical trapping ensures to keep a safe distance between the trapping
beams and the sensitive cell and enables their 6 degrees of freedom actuation. (C)2015
Optical Society of America
