The advent of capillary electrophoresis (CE) , with its power to separate and analyze
very small amounts of DNA, has generated a host of DNA analytical procedures. In these
novel and wide-ranging volumes of Capillary Electrophoresis of Nucleic Acids, an outstanding
panel of hands-on experts and developers of CE equipment describe in step-by-step
fashion their best cutting-edge methods for the detection and analysis of DNA mutations
and modifications, ranging from precise DNA loci to entire genomes of organisms. This
first volume of the set, Introduction to the Capillary Electrophoresis of Nucleic
Acids, covers the practical and theoretical considerations behind the use of capillary
electrophoresis for the analysis of small oligonucleotides and modified nucleotides.
Along with detailed instructions ensuring ready reproducibility, these protocols offer
time-tested advice on instrumentation, signal detection, the capillary environment,
and the integration of mass spectrometry with CE. Several chapters are devoted to
the analysis of small therapeutic oligonucleotides, nucleosides, and ribonucleotides
by CE. The companion volume, Practical Applications of Capillary Electrophoresis,
addresses techniques for high-throughput analysis of DNA fragments using SNP detection,
mutation detection, DNA sequencing methods, and DNA-ligand interactions.
Comprehensive and up-to-date, the paired volumes of Capillary Electrophoresis of Nucleic
Acids offer an authoritative guide with easy access to fast, versatile, reliable,
and powerful technologies for all those basic and clinical investigators analyzing
DNA variation today.
