FEMTO3D Atlas(FEMTO3D Atlas három dimenzióban pásztázó mikroszkóp (Infrastruktúra))
Funder: FEMTONICS
Subjects:
Basic medicine
Biological sciences
Clinical medicine
Communication between individual GABAergic cells and their target neurons is mediated
by synapses and, in the case of neurogliaform cells (NGFCs), by unitary volume transmission.
Effects of non-synaptic volume transmission might involve non-neuronal targets, and
astrocytes not receiving GABAergic synapses but expressing GABA receptors are suitable
for evaluating this hypothesis. Testing several cortical interneuron types in slices
of the rat cerebral cortex, we show selective unitary transmission from NGFCs to astrocytes
with an early, GABAA receptor and GABA transporter-mediated component and a late component
that results from the activation of GABA transporters and neuronal GABAB receptors.
We could not detect Ca2+ influx in astrocytes associated with unitary GABAergic responses.
Our experiments identify a presynaptic cell-type-specific, GABA-mediated communication
pathway from individual neurons to astrocytes, assigning a role for unitary volume
transmission in the control of ionic and neurotransmitter homeostasis.
