https://m2.mtmt.hu/ eng 2026-10-24 06:59 JournalArticle 3012680 VALIDATED true Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvárad tér 4, Budapest, 1089, Hungary Department of Otorhinolaryngology, Head and Neck Surgery, Bajcsy-Zsilinszky Hospital, Budapest, Hungary Department of Otorhinolaryngology, Head and Neck Surgery, Semmelweis University, Budapest, Hungary Program in Neurosciences and Mental Health, The Hospital for Sick Children, Toronto, ON, Canada Department of Otolaryngology, Head and Neck Surgery, University Medical Center Groningen, University of Groningen, Groningen, Netherlands Pharmacological and Drug Safety Research, Gedeon Richter Plc, Budapest, Hungary Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary Computational Cognitive Neuroimaging Laboratory, Computational Neuroscience and Cognitive Robotics Centre, University of Birmingham, Birmingham, United Kingdom Cited By :11 Export Date: 9 November 2022 CODEN: NERED Correspondence Address: Zelles, T.; Department of Pharmacology and Pharmacotherapy, Nagyvárad tér 4, Hungary; email: zelles.tibor@med.semmelweis-univ.hu Chemicals/CAS: adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; calcium, 7440-70-2, 14092-94-5; cyclopiazonic acid, 18172-33-3, 83136-88-3; fura 2 acetoxymethyl ester, 105344-37-4, 108964-32-5; pyridoxal phosphate 6 azophenyl 2',4' disulfonic acid, 149017-66-3; Adenosine Triphosphate; Receptors, Purinergic P2X; Receptors, Purinergic P2Y; RNA, Messenger Funding details: K116654, NN107234 Funding details: TÉT_10-1-2011-0421 Funding text 1: This work was supported by the Hungarian-French Collaborative R&I Programme on Biotechnologies (TÉT_10-1-2011-0421) and the Hungarian Research and Development Fund (NN107234 and K116654). We thank Peter Dallos and Claus-Peter Richter for teaching us the preparation of the hemicochlea and László Köles for his advices concerning purinergic receptor pharmacology. 0 2018-09-26T09:44:38.000+0000 false 3012680 2026-10-17T13:26:02.253+0000 2024-06-11T12:14:25.550+0000 2016-02-03T11:29:54.000+0000 true 10008942 Sperlágh Beáta /api/author/10008942 Author true 10008942 2026-01-12T09:29:34.545+0000 2026-01-12T09:29:34.545+0000 2022-10-09T12:24:03.940+0000 true 10015544 Csajbók Edit /api/admin/10015544 Admin true 10015544 Admin 10066925 /api/admin/10066925 Nemec Évi true true Admin 10067876 /api/admin/10067876 Ladányi Gusztáv true true 2024-10-17T16:20:19.023+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Horvath, T ATP-Evoked Intracellular Ca Signaling of Different Supporting Cells in the Hearing Mouse Hemicochlea. true true /api/journal/3344 REVIEWED true false 3344 false 3344 true 0364-3190 1573-6903 Journal FOREIGN 41 1-2 364 375 364 12 2016 Hearing and its protection is regulated by ATP-evoked Ca2+ signaling in the supporting cells of the organ of Corti, however, the unique anatomy of the cochlea hampers observing these mechanisms. For the first time, we have performed functional ratiometric Ca2+ imaging (fura-2) in three different supporting cell types in the hemicochlea preparation of hearing mice to measure purinergic receptor-mediated Ca2+ signaling in pillar, Deiters' and Hensen's cells. Their resting [Ca2+]i was determined and compared in the same type of preparation. ATP evoked reversible, repeatable and dose-dependent Ca2+ transients in all three cell types, showing desensitization. Inhibiting the Ca2+ signaling of the ionotropic P2X (omission of extracellular Ca2+) and metabotropic P2Y purinergic receptors (depletion of intracellular Ca2+ stores) revealed the involvement of both receptor types. Detection of P2X2,3,4,6,7 and P2Y1,2,6,12,14 receptor mRNAs by RT-PCR supported this finding and antagonism by PPADS suggested different functional purinergic receptor population in pillar versus Deiters' and Hensen's cells. The sum of the extra- and intracellular Ca2+-dependent components of the response was about equal with the control ATP response (linear additivity) in pillar cells, and showed supralinearity in Deiters' and Hensen's cells. Calcium-induced calcium release might explain this synergistic interaction. The more pronounced Ca2+ leak from the endoplasmic reticulum in Deiters' and Hensen's cells, unmasked by cyclopiazonic acid, may also suggests the higher activity of the internal stores in Ca2+ signaling in these cells. Differences in Ca2+ homeostasis and ATP-induced Ca2+ signaling might reflect the distinct roles these cells play in cochlear function and pathophysiology. true true 2016 672516 true true true false true false GREEN GREEN 2026-10-17 true https://real.mtak.hu/52200 true 10015544 Csajbók Edit /api/admin/10015544 Admin true 10015544 21 0 16 12 6 18 15 13 14 15 15 11 11 17 4 0 21 17 16 12 0 6 6 Folyóiratcikk Journal Article 24 16 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 4 Egyéb Miscellaneous 29 1 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2016 0 2 2 2 2 2017 0 1 1 1 1 2018 0 2 2 2 2 2019 0 6 2 6 2 2020 0 4 4 4 4 2021 0 1 1 1 1 2022 0 3 3 3 3 2023 0 1 1 1 1 2024 0 0 0 0 0 2025 0 1 1 1 1 Q1 false 10064479 false false false 2016-06-07T12:22:23.000+0000 2016-03-09T13:22:23.000+0000 Farmakológiai Kutatócsoport (KOKI / GYO); Farmakológiai és Farmakoterápiás Intézet (SE / AOK / I); Fül-Orr-Gégészeti és Fej-Nyaksebészeti Klinika (SE / AOK / K); Molekuláris Farmakológia Kutatócsoport (KOKI / GYO) 8 29 4 10 0 true 10066925 10067876 Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 7449817 /api/authorship/7449817 1 0.1 true false Author 10064479 /api/author/10064479 Horváth Tamás true 10064479 true Horvath T true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 23451794 true PersonAuthorship 7449818 /api/authorship/7449818 2 0.1 false false Author 221 /api/author/221 Polony Gábor true 221 true Polony G true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 23451793 true PersonAuthorship 7449819 /api/authorship/7449819 3 0.1 false false Fekete A true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 23451792 true PersonAuthorship 7449820 /api/authorship/7449820 4 0.1 false false Author 10026065 /api/author/10026065 Aller Máté true 10026065 true Aller M true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 23451791 true PersonAuthorship 7449821 /api/authorship/7449821 5 0.1 false false Halmos G true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 23451790 true PersonAuthorship 7449822 /api/authorship/7449822 6 0.1 false false Author 10010479 /api/author/10010479 Lendvai Balázs true 10010479 true Lendvai B true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 23451789 true PersonAuthorship 7449823 /api/authorship/7449823 7 0.1 false false Author 229 /api/author/229 Heinrich Attila true 229 true Heinrich A true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 23451788 true PersonAuthorship 7449824 /api/authorship/7449824 8 0.1 false false Author 10008942 /api/author/10008942 Sperlágh Beáta true 10008942 true Sperlagh B true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 23451787 true PersonAuthorship 7449825 /api/authorship/7449825 9 0.1 false false Author 10000023 /api/author/10000023 Vizi E. 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<span class="author-name" mtid="221"><a href="/gui2/?type=authors&mode=browse&sel=221" target="_blank">Polony G (<span class="authorship-author-name">Polony Gábor</span> <span class="authorAux-mtmt"> Fül-orr-gégészet</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Clinical">C</span>/Department of Otorhinolaryngology, Head and Neck Surgery</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Fekete A </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10026065"><a href="/gui2/?type=authors&mode=browse&sel=10026065" target="_blank">Aller M (<span class="authorship-author-name">Aller Máté</span> <span class="authorAux-mtmt"> Celluláris farmakológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Instituties">I</span>/Department of Pharmacology and Pharmacotherapy</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Halmos G </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10010479"><a href="/gui2/?type=authors&mode=browse&sel=10010479" target="_blank">Lendvai B (<span class="authorship-author-name">Lendvai Balázs</span> <span class="authorAux-mtmt"> Neurofarmakológia</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="229"><a href="/gui2/?type=authors&mode=browse&sel=229" target="_blank">Heinrich A (<span class="authorship-author-name">Heinrich Attila</span> <span class="authorAux-mtmt"> Farmakológia</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10008942"><a href="/gui2/?type=authors&mode=browse&sel=10008942" target="_blank">Sperlagh B (<span class="authorship-author-name">Sperlágh Beáta</span> <span class="authorAux-mtmt"> Neurofarmakológia</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Pharmacology">DP</span>/Laboratory of Molecular Pharmacology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000023"><a href="/gui2/?type=authors&mode=browse&sel=10000023" target="_blank">Vizi ES (<span class="authorship-author-name">Vizi E. Szilveszter</span> <span class="authorAux-mtmt"> Neurofarmakológia, neurokémia</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Pharmacology">DP</span>/Laboratory of Drug Research</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000028"><a href="/gui2/?type=authors&mode=browse&sel=10000028" target="_blank">Zelles T ✉ (<span class="authorship-author-name">Zelles Tibor</span> <span class="authorAux-mtmt"> Idegtudományok</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Pharmacology">DP</span>/Laboratory of Drug Research; <span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Instituties">I</span>/Department of Pharmacology and Pharmacotherapy</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;3012680" target="_blank">ATP-Evoked Intracellular Ca Signaling of Different Supporting Cells in the Hearing Mouse Hemicochlea.</a></div> <div> <span class="journal-title">NEUROCHEMICAL RESEARCH</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0364-3190">0364-3190</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1573-6903">1573-6903</a>)</span>: <span class="journal-volume">41</span> <span class="journal-issue">1-2</span> <span class="page"> pp 364-375 </span> <span class="year">(2016)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.1007/s11064-015-1818-4" target="_blank" href="https://doi.org/10.1007/s11064-015-1818-4"> DOI </a> </span> <span class="id identifier oa_GREEN" title=" Green "> <a style="color:black" title="52200" target="_blank" href="https://real.mtak.hu/52200"> REAL </a> </span> <span class="id identifier oa_GREEN" title=" Green "> <a style="color:blue" title="6307" target="_blank" href="https://repo.lib.semmelweis.hu/handle/123456789/6307"> SE Repozitórium </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000371412800035" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000371412800035"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="84955266033" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84955266033"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="26801171" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=26801171&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> <div class="journal-subject">Journal subject: Scopus - Biochemistry&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> <div class="journal-subject">Journal subject: Scopus - Cellular and Molecular Neuroscience&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q3</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 21</span> | Independent citation: 17 | Self citation: 4 | Unknown citation: 0 | Number of citations in WoS: 13 | Number of citations in Scopus:&nbsp;14 | WoS/Scopus assigned:&nbsp;15 | Number of citations with DOI:&nbsp;15 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;3012680&sort=publishedYear,desc&sort=title"> Number of cited publications: 6 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 3012680</span> | <span class="status-data status-VALIDATED"> Validated </span> <span class="oldId">Old id: 3012680</span> | Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">MEDLINE</span> </div> <div class="lastModified">Last Modified: 2024.06.11. 14:14 Magdolna Klára Teknős-Abonyi (NSZKK 4 admin) </div> <div class="lockedBy">Centrally managed 2024.10.17. 18:20 Évi Nemec (MTMT Központi 3, admin) Gusztáv Ladányi (MTMT API user, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Comments</u>: Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvárad tér 4, Budapest, 1089, Hungary Department of Otorhinolaryngology, Head and Neck Surgery, Bajcsy-Zsilinszky Hospital, Budapest, Hungary Department of Otorhinolaryngology, Head and Neck Surgery, Semmelweis University, Budapest, Hungary Program in...</pre> </div></div>