https://m2.mtmt.hu/ eng 2026-10-25 00:12 JournalArticle 3001556 VALIDATED true Barna László és Dudok Barna megosztott első szerzők. Momentum Laboratory of Molecular Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary School of Ph.D. Studies, Semmelweis University, Budapest, Hungary Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Budapest, Hungary Cited By :31 Export Date: 31 March 2021 Correspondence Address: Barna, L.; Momentum Laboratory of Molecular Neurobiology, Hungary; email: katona@koki.hu Funding details: Wellcome Trust, WT Funding details: European Research Council, ERC, 243153 Funding details: Magyar Tudományos Akadémia, MTA, LP-54/2013 Funding text 1: acknoWleDGMents The authors are grateful to B. Pintér for technical assistance and help with establishing the tissue handling protocol; E. Tischler for technical assistance; and E. Szabadits and R. Nyilas (Institute of Experimental Medicine, Hungarian Academy of Sciences) for help with optimizing the immunostaining. We also thank M. Ledri (Institute of Experimental Medicine, Hungarian Academy of Sciences) for providing brain samples, I. Kacskovics (Immunogenes, Ltd.) and G. Balla (Institute of Experimental Medicine, Hungarian Academy of Sciences) for providing antibodies, and A. Zimmer (Institute of Molecular Psychiatry, University of Bonn) for providing knockout mice. We are grateful to S. Turbucz for his help with coding the software. We acknowledge the help of the Nikon Microscopy Center at the Institute of Experimental Medicine, Nikon Europe, Nikon Austria and Auro-Science Consulting for kindly providing microscopy support. The authors are indebted to I. Sase and I. Burda from Nikon for their invaluable help with the application of N-STORM. This work was supported by the Hungarian Academy of Sciences Momentum Program LP-54/2013, by the European Research Council Starting grant no. 243153 and by the Wellcome Trust International Senior Research Fellowship (to I.K.). Momentum Laboratory of Molecular Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary School of Ph.D. Studies, Semmelweis University, Budapest, Hungary Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Budapest, Hungary Cited By :31 Export Date: 12 April 2021 Correspondence Address: Barna, L.; Momentum Laboratory of Molecular Neurobiology, Hungary; email: katona@koki.hu Funding details: Wellcome Trust, WT Funding details: European Research Council, ERC, 243153 Funding details: Magyar Tudományos Akadémia, MTA, LP-54/2013 Funding text 1: acknoWleDGMents The authors are grateful to B. Pintér for technical assistance and help with establishing the tissue handling protocol; E. Tischler for technical assistance; and E. Szabadits and R. Nyilas (Institute of Experimental Medicine, Hungarian Academy of Sciences) for help with optimizing the immunostaining. We also thank M. Ledri (Institute of Experimental Medicine, Hungarian Academy of Sciences) for providing brain samples, I. Kacskovics (Immunogenes, Ltd.) and G. Balla (Institute of Experimental Medicine, Hungarian Academy of Sciences) for providing antibodies, and A. Zimmer (Institute of Molecular Psychiatry, University of Bonn) for providing knockout mice. We are grateful to S. Turbucz for his help with coding the software. We acknowledge the help of the Nikon Microscopy Center at the Institute of Experimental Medicine, Nikon Europe, Nikon Austria and Auro-Science Consulting for kindly providing microscopy support. The authors are indebted to I. Sase and I. Burda from Nikon for their invaluable help with the application of N-STORM. This work was supported by the Hungarian Academy of Sciences Momentum Program LP-54/2013, by the European Research Council Starting grant no. 243153 and by the Wellcome Trust International Senior Research Fellowship (to I.K.). 0 2018-05-11T12:05:37.000+0000 false 3001556 2026-10-17T12:17:22.256+0000 2020-12-17T11:04:03.278+0000 2016-01-20T06:37:20.000+0000 true 10011502 Katona István /api/author/10011502 Author true 10011502 2025-03-24T14:06:19.445+0000 2025-03-24T14:06:19.445+0000 2019-11-07T09:00:04.564+0000 true 10062062 Gere Tamásné /api/admin/10062062 Admin true 10062062 2020-12-17T11:04:03.288+0000 true 10062250 Molnár-Taga Márta /api/admin/10062250 Admin true 10062250 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Barna, L Correlated confocal and super-resolution imaging by VividSTORM true true /api/journal/10009104 REVIEWED true false 10009104 false 10009104 true 1754-2189 1750-2799 Journal FOREIGN 11 1 163 183 163 21 2016 Single-molecule localization microscopy (SMLM) is rapidly gaining popularity in the life sciences as an efficient approach to visualize molecular distribution with nanoscale precision. However, it has been challenging to obtain and analyze such data within a cellular context in tissue preparations. Here we describe a 5-d tissue processing and immunostaining procedure that is optimized for SMLM, and we provide example applications to fixed mouse brain, heart and kidney tissues. We then describe how to perform correlated confocal and 3D-superresolution imaging on these sections, which allows the visualization of nanoscale protein localization within labeled subcellular compartments of identified target cells in a few minutes. Finally, we describe the use of VividSTORM (http://katonalab.hu/index.php/vividstorm), an open-source software for correlated confocal and SMLM image analysis, which facilitates the measurement of molecular abundance, clustering, internalization, surface density and intermolecular distances in a cell-specific and subcellular compartment-restricted manner. The protocol requires only basic skills in tissue staining and microscopy. true true 2016 803179 true true true false true false GOLD 2026-10-17 true https://doi.org/10.1038/nprot.2016.002 true 10063958 Horváth Eszter /api/admin/10063958 Admin true 10063958 100 0 70 57 23 73 66 60 60 63 62 51 50 81 19 0 100 81 70 57 0 8 8 Folyóiratcikk Journal Article 24 64 Könyvrészlet Chapter in Book 25 4 Könyv Book 23 2 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 11 Egyéb Miscellaneous 29 19 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2016 0 5 4 5 4 2017 0 18 16 18 16 2018 0 16 14 16 14 2019 0 13 11 13 11 2020 0 7 5 7 5 2021 0 11 8 11 8 2022 0 14 11 14 11 2023 0 6 5 6 5 2024 0 6 4 6 4 2025 0 2 2 2 2 2026 0 2 1 2 1 D1 false 10011502,10029872,10052396 false false false 2016-04-19T05:40:15.000+0000 2016-01-20T06:40:15.000+0000 Doktori Iskola (SE); Információs Technológiai és Bionikai Kar (PPKE); Molekuláris Neurobiológiai Kutatócsoport (HRN KOKI); Molekuláris Neurobiológiai Kutatócsoport (MTAKOKI / GFO); Roska Tamás Műszaki és Természettudományi Dokto... 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D. Studies</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10052396"><a href="/gui2/?type=authors&mode=browse&sel=10052396" target="_blank">Miczan V (<span class="authorship-author-name">Csapóné Miczán Vivien</span> <span class="authorAux-mtmt"> Képalkotás</span>) </a> </span> <span class="author-affil"><span title="Pázmány Péter Catholic University">PPCU</span>/<span title="Információs Technológiai és Bionikai Kar">ITK</span>/Roska Tamás Műszaki és Természettudományi Doktori Iskola</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10029872"><a href="/gui2/?type=authors&mode=browse&sel=10029872" target="_blank">Horvath A (<span class="authorship-author-name">Horváth András</span> <span class="authorAux-mtmt"> mérnöki tudományok</span>) </a> </span> <span class="author-affil"><span title="Pázmány Péter Catholic University">PPCU</span>/Információs Technológiai és Bionikai Kar</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10055721"><a href="/gui2/?type=authors&mode=browse&sel=10055721" target="_blank">Laszlo ZI (<span class="authorship-author-name">László Zsófia Ilona</span> <span class="authorAux-mtmt"> Neurobiológia</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Molekuláris és Fejlődés Neurobiológia Osztály">MFNO</span>/Molekuláris Neurobiológiai Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10011502"><a href="/gui2/?type=authors&mode=browse&sel=10011502" target="_blank">Katona I ✉ (<span class="authorship-author-name">Katona István</span> <span class="authorAux-mtmt"> Neurobiológia</span>) </a> </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;3001556" target="_blank">Correlated confocal and super-resolution imaging by VividSTORM</a></div> <div> <span class="journal-title">NATURE PROTOCOLS</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1754-2189">1754-2189</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1750-2799">1750-2799</a>)</span>: <span class="journal-volume">11</span> <span class="journal-issue">1</span> <span class="page"> pp 163-183 </span> <span class="year">(2016)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1038/nprot.2016.002" target="_blank" href="https://doi.org/10.1038/nprot.2016.002"> DOI </a> </span> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="3801" target="_blank" href="https://publikacio.ppke.hu/id/eprint/3801"> PPCE Publications </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000367462100011" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000367462100011"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="84988566258" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84988566258"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="26716705" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=26716705&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Biochemistry, Genetics and Molecular Biology (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;D1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 100</span> | Independent citation: 81 | Self citation: 19 | Unknown citation: 0 | Number of citations in WoS: 60 | Number of citations in Scopus:&nbsp;60 | WoS/Scopus assigned:&nbsp;63 | Number of citations with DOI:&nbsp;66 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;3001556&sort=publishedYear,desc&sort=title"> Number of cited publications: 8 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 3001556</span> | <span class="status-data status-VALIDATED"> Validated </span> <span class="oldId">Old id: 3001556</span> | Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">MEDLINE</span> </div> <div class="lastModified">Last Modified: 2020.12.17. 12:04 Márta Molnár-Taga (SE 4-es admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Comments</u>: Barna László és Dudok Barna megosztott első szerzők. Momentum Laboratory of Molecular Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary School of Ph.D. Studies, Semmelweis University, Budapest, Hungary Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Budapest, Hungar...</pre> </div></div>