Involvement of small heat shock proteins, trehalose, and lipids in the thermal stress management in Schizosaccharomyces pombe.

Glatz, A [Glatz, Attila (biokémia), szerző] Biokémiai Intézet (MTA SZBK); Pilbat, AM [Pilbat, Ana Maria (biofizika), szerző] Biokémiai Intézet (MTA SZBK); Nemeth, GL; Vince-Kontar, K; Josvay, K [Jósvay, Katalin (molekuláris biológia), szerző] Biokémiai Intézet (MTA SZBK); Hunya, A [Hunya, Ákos (neurobiológia), szerző] Biokémiai Intézet (MTA SZBK); Udvardy, A [Udvardy, Andor (Biokémia), szerző] Biokémiai Intézet (MTA SZBK); Gombos, I [Gombos, Imre (Immunológia, biok...), szerző] Biokémiai Intézet (MTA SZBK); Peter, M [Péter, Mária (biokémia), szerző]; Balogh, G [Balogh, Gábor (biokémia), szerző] Biokémiai Intézet (MTA SZBK); Horvath, I [Horváth, Ibolya (Biokémia, stressz...), szerző] Biokémiai Intézet (MTA SZBK); Vigh, L [Vigh, László (Membránbiológia), szerző] Biokémiai Intézet (MTA SZBK); Torok, Z [Török, Zsolt (Membrán biofizika), szerző] Biokémiai Intézet (MTA SZBK)

Angol nyelvű Tudományos Szakcikk (Folyóiratcikk)
Megjelent: CELL STRESS & CHAPERONES 1355-8145 1466-1268 21 (2) pp. 327-338 2016
  • SJR Scopus - Biochemistry: Q2
    Changes in the levels of three structurally and functionally different important thermoprotectant molecules, namely small heat shock proteins (sHsps), trehalose, and lipids, have been investigated upon heat shock in Schizosaccharomyces pombe. Both alpha-crystallin-type sHsps (Hsp15.8 and Hsp16) were induced after prolonged high-temperature treatment but with different kinetic profiles. The shsp null mutants display a weak, but significant, heat sensitivity indicating their importance in the thermal stress management. The heat induction of sHsps is different in wild type and in highly heat-sensitive trehalose-deficient (tps1Delta) cells; however, trehalose level did not show significant alteration in shsp mutants. The altered timing of trehalose accumulation and induction of sHsps suggest that the disaccharide might provide protection at the early stage of the heat stress while elevated amount of sHsps are required at the later phase. The cellular lipid compositions of two different temperature-adapted wild-type S. pombe cells are also altered according to the rule of homeoviscous adaptation, indicating their crucial role in adapting to the environmental temperature changes. Both Hsp15.8 and Hsp16 are able to bind to different lipids isolated from S. pombe, whose interaction might provide a powerful protection against heat-induced damages of the membranes. Our data suggest that all the three investigated thermoprotectant macromolecules play a pivotal role during the thermal stress management in the fission yeast.
    Hivatkozás stílusok: IEEEACMAPAChicagoHarvardCSLMásolásNyomtatás
    2020-08-03 23:32