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The aim of this study was: (1) to determine the surface-electromyography-root-mean-square (sEMGRMS)-acceleration load relationship in the vastus lateralis (VL), vastus medialis (VM), rectus femoris (RF), lateral gastrocnemius (LG) muscles during synchronous WBV, and (2) to assess the reliability of the acceleration corresponding to the maximal sEMGRMS. Methods: Twenty-five sportsman voluntarily took part in this study with a single-group, repeated-measures design. All subjects postured themselves in an isometric half-squat during nine trials in the following conditions: no vibrations and random vibrations of different acceleration loads (from 0.12 to 5.72g). Results: The sEMGRMS were dependent on the acceleration loads in the VL (p=0.0001), LG (p=0.0001) and VM (p=0.011) muscles; while RF was not affected by the acceleration loads (p=0.508). The comparisons among the sEMGRMS-accelerations relationships revealed a significant difference between the LG and the others muscles (p=0.001). 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