https://m2.mtmt.hu/ eng 2026-10-23 18:03 JournalArticle 2986910 VALIDATED true 0 2018-02-20T16:43:57.000+0000 false 2986910 2026-10-17T11:20:01.133+0000 2025-09-02T07:37:44.447+0000 2015-12-21T14:15:45.000+0000 true 10011473 Gereben Balázs /api/author/10011473 Author true 10011473 2025-11-11T08:58:43.162+0000 2025-11-11T08:58:43.162+0000 2025-09-02T07:37:44.538+0000 true 10026589 Tichy Rita /api/admin/10026589 Admin true 10026589 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Kollár, A Different Types of Luciferase Reporters Show Distinct Susceptibility to T3-Evoked Downregulation true true /api/journal/4276 REVIEWED true false 4276 false 4276 true 1050-7256 1557-9077 Journal FOREIGN 26 1 179 182 179 4 2016 BACKGROUND: The firefly luciferase reporter protein is a crucial tool for studies targeting a broad range of biological questions. Importantly, luciferase assays are also widely used to explore mechanisms underlying thyroid hormone dependent regulation of gene expression. However, it was demonstrated that the firefly luciferase reporter is subject to triiodothyronine (T3)-evoked, promoter independent downregulation that is mediated by the thyroid hormone receptor. Since this effect can interfere with readout accuracy, the study aimed to find luciferase reporters that are not susceptible to this phenomenon. METHODS: Luciferase reporter constructs were generated under the control of a minimal thymidine kinase (TK) promoter and transiently transfected into JEG-3 cells to test their activity upon T3 treatment. RESULTS: Activity of the TK-(dCpG)Luc encoding a synthetic (dCpG)Luciferase and TK-NanoLuc expressing the NanoLuc reporter was not significantly changed by T3 treatment while the firefly luciferase control was suppressed by approximately 2.6-fold. T3 also downregulated the activity of Renilla luciferase by approximately 30%. CONCLUSIONS: Novel types of luciferase reporters, especially the synthetic (dCpG)Luciferase, can be more accurate to study T3-regulated gene expression than the classical firefly luciferase reporter. Renilla luciferase, a popular transfection control of dual luciferase assays, should be used with caution in conditions with T3 treatment. true true 2015 794547 true true true false true false GOLD 2026-10-17 true https://doi.org/10.1089/thy.2015.0398 true 10030885 Bálint Eszter /api/admin/10030885 Admin true 10030885 3 0 3 2 1 3 3 3 3 3 3 2 2 2 1 0 3 2 3 2 0 3 3 Folyóiratcikk Journal Article 24 3 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2018 0 1 0 1 0 2019 0 0 0 0 0 2020 0 2 2 2 2 D1 false false false false 2016-05-26T05:18:41.000+0000 2016-02-26T06:18:41.000+0000 Molekuláris Sejt Metabolizmus Kutatócsoport (HRN KOKI) 4 27 1 4 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 6921115 /api/authorship/6921115 1 0.25 true false Author 10057867 /api/author/10057867 Kollár Anna true 10057867 true Kollár A true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 22670016 true PersonAuthorship 6921116 /api/authorship/6921116 2 0.25 false false Author 10090216 /api/author/10090216 Papp Zsuzsanna true true Kvárta-Papp Z true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 22670015 true PersonAuthorship 6921117 /api/authorship/6921117 3 0.25 false false Author 206 /api/author/206 Egri Péter true 206 true Egri P true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 22670014 true PersonAuthorship 6921118 /api/authorship/6921118 4 0.25 false true true Author 10011473 /api/author/10011473 Gereben Balázs true 10011473 true Gereben B true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 22670013 true PublicationIdentifier 1046984 /api/publicationidentifier/1046984 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true IDENTICAL 10.1089/thy.2015.0398 https://doi.org/10.1089/thy.2015.0398 false 1270588 true PublicationIdentifier 14967077 /api/publicationidentifier/14967077 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000368022200009 https://www.webofscience.com/wos/woscc/full-record/000368022200009 false 11335543 true PublicationIdentifier 1046983 /api/publicationidentifier/1046983 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true false IDENTICAL 84954531197 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84954531197 false 1725909 true PublicationIdentifier 1046985 /api/publicationidentifier/1046985 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true false IDENTICAL 26629840 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=26629840&dopt=Abstract false 1270589 true Classification 10545 /api/classification/10545 true true Classification 12548 /api/classification/12548 true true Classification 12215 /api/classification/12215 true true SjrRating 10946825 /api/sjrrating/10946825 211 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 2701 /api/classificationexternal/2701 true 2701 true D1 DIRECT true true /api/publication/2986910 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10057867"><a href="/gui2/?type=authors&mode=browse&sel=10057867" target="_blank">Kollár A (<span class="authorship-author-name">Kollár Anna</span> <span class="authorAux-mtmt"> Neuroendokrinológia</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10090216"><a href="/gui2/?type=authors&mode=browse&sel=10090216" target="_blank">Kvárta-Papp Z (<span class="authorship-author-name">Papp Zsuzsanna</span> <span class="authorAux-mtmt"> Molekuláris biológia, gyulladás élettan</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="206"><a href="/gui2/?type=authors&mode=browse&sel=206" target="_blank">Egri P (<span class="authorship-author-name">Egri Péter</span> <span class="authorAux-mtmt"> Endokrinológia</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Endocrine Neurobiology">DEN</span>/Laboratory of Molecular Cell Metabolism</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10011473"><a href="/gui2/?type=authors&mode=browse&sel=10011473" target="_blank">Gereben B ✉ (<span class="authorship-author-name">Gereben Balázs</span> <span class="authorAux-mtmt"> Pajzsmirigy endokrinológia</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Endocrine Neurobiology">DEN</span>/Laboratory of Molecular Cell Metabolism</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;2986910" target="_blank">Different Types of Luciferase Reporters Show Distinct Susceptibility to T3-Evoked Downregulation</a></div> <div> <span class="journal-title">THYROID</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1050-7256">1050-7256</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1557-9077">1557-9077</a>)</span>: <span class="journal-volume">26</span> <span class="journal-issue">1</span> <span class="page"> pp 179-182 </span> <span class="year">(2016)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1089/thy.2015.0398" target="_blank" href="https://doi.org/10.1089/thy.2015.0398"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000368022200009" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000368022200009"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="84954531197" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84954531197"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="26629840" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=26629840&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Medicine (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;D1</div> <div class="journal-subject">Journal subject: Scopus - Endocrinology&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> <div class="journal-subject">Journal subject: Scopus - Endocrinology, Diabetes and Metabolism&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 3</span> | Independent citation: 2 | Self citation: 1 | Unknown citation: 0 | Number of citations in WoS: 3 | Number of citations in Scopus:&nbsp;3 | WoS/Scopus assigned:&nbsp;3 | Number of citations with DOI:&nbsp;3 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;2986910&sort=publishedYear,desc&sort=title"> Number of cited publications: 3 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 2986910</span> | <span class="status-data status-VALIDATED"> Validated </span> <span class="oldId">Old id: 2986910</span> | Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">MEDLINE</span> </div> <div class="lastModified">Last Modified: 2025.09.02. 09:37 Rita Tichy (SE_KK_Admin5_TR, admin) </div> </div></div>