https://m2.mtmt.hu/ hun 2026-04-05 11:19 JournalArticle 2974496 VALIDATED true : 10.1016/j.ijfoodmicro.2011.07.011. Epub 2011 Aug 4. PMC PMC2708511 Hiányzó Besorolás: 'Evaluation Studies\n\nJournal Article\n\nResearch Support, Non-U.S. Gov\'t',24 Megjegyzés-23107078 N1 Molecular Sequence Numbers: GENBANK: AF217084, AF309418, AJ629062, AJ880277, AY562991, DQ097394, EF201805, EU604247, EU604248, EU604249, EU604250, EU604251, EU604252, EU604253, EU604254, EU604255, EU604256, EU604257, EU604258, EU604259, EU604260, EU604261, EU604262, EU604263, EU604264, EU604265, EU604266, EU604267, EU604268, EU604269, EU604270, EU604271, EU604814, EU665683, FJ687486, FJ687487, FJ687488, FJ687489, X04719; N1 : Chemicals/CASDNA Primers; RNA, Viral; Viral Fusion Proteins 0 2016-04-25T13:56:14.000+0000 false 2974496 2025-12-24T13:13:19.361+0000 2025-09-20T10:56:21.277+0000 2015-11-24T08:59:20.000+0000 true 10052149 Farkas Tibor /api/author/10052149 Author true 10052149 2025-09-20T10:56:21.010+0000 2020-09-01T07:09:05.999+0000 true 565 WoS import /api/admin/565 Admin true true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Farkas, T Real-time PCR-based pathotyping of Newcastle disease virus by use of TaqMan minor groove binder probes. true true /api/journal/2369 REVIEWED true false 2369 false 2369 true 0095-1137 1098-660X Journal FOREIGN 47 7 2114 2123 2114 10 2009 A real-time reverse-transcription PCR was developed to detect and pathotype Newcastle disease viruses (NDV) in clinical samples. Degenerate oligonucleotide primers and TaqMan probes with nonfluorescent minor groove binder (MGB) quencher amplified and hybridized to a region in the fusion protein (F) gene that corresponds to the cleavage site of the F0 precursor, which is a key determinant of NDV pathogenicity. The application of degenerate primers and TaqMan MGB probes provided high specificity to the assay, as was shown by the successful and rapid pathotype determination of 39 NDV strains representing all the known genotypes (I to VIII) and pathotypes (lentogens/mesogens/velogens). The PCR assays specific for lentogenic and velogenic/mesogenic strains had high analytical sensitivity, detecting approximately 10 and 20 copies of the target molecule per reaction, respectively. The detection limit was also determined in terms of 50% egg infective dose (EID(50)) by using dilution series of virus stock solutions to be approximately 10(1.0) and 10(-1.3) EID(50)/ml for lentogens and velogens/mesogens, respectively. Organ, swab, and stool specimens from experimentally infected animals were tested to prove the clinical suitability of the method. The results of this study suggest that the described real-time PCR assay has the potential to be used for the rapid detection/pathotyping of NDV isolates and qualitative/quantitative measurement of the virus load. 2015 861095 true true true false false NONE 2025-12-24 false true 10014568 Vándor Andrea /api/admin/10014568 Admin true 10014568 35 0 35 33 0 35 33 35 17 35 35 33 33 33 2 0 35 33 35 33 0 8 8 Folyóiratcikk Journal Article 24 35 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció 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false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 22473194 true PersonAuthorship 6833483 /api/authorship/6833483 4 0.25 false true Author 10009849 /api/author/10009849 Kiss István true 10009849 true Kiss I true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 22473193 true PublicationIdentifier 1038907 /api/publicationidentifier/1038907 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true false IDENTICAL 10.1128/JCM.01652-08 https://doi.org/10.1128/JCM.01652-08 false 1256961 true PublicationIdentifier 15053240 /api/publicationidentifier/15053240 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000267713000018 https://www.webofscience.com/wos/woscc/full-record/000267713000018 false 2966364 true PublicationIdentifier 14884317 /api/publicationidentifier/14884317 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 67650021215 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-67650021215 false 3256646 true PublicationIdentifier 1038908 /api/publicationidentifier/1038908 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true false IDENTICAL 19439542 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=19439542&dopt=Abstract false 1256962 true Keyword 12 /api/keyword/12 true 12 true Keyword 4219 /api/keyword/4219 true 4219 true Keyword 4442 /api/keyword/4442 true 4442 true Keyword 4944 /api/keyword/4944 true 4944 true Keyword 5220 /api/keyword/5220 true 5220 true Keyword 1003512 /api/keyword/1003512 true 1003512 true Keyword 1014756 /api/keyword/1014756 true 1014756 true Keyword 1038174 /api/keyword/1038174 true 1038174 true Keyword 1126251 /api/keyword/1126251 true 1126251 true Keyword 1165353 /api/keyword/1165353 true 1165353 true Keyword 1385477 /api/keyword/1385477 true 1385477 true Keyword 1385478 /api/keyword/1385478 true 1385478 true Keyword 1385479 /api/keyword/1385479 true 1385479 true SjrRating 5810131 /api/sjrrating/5810131 8 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 2726 /api/classificationexternal/2726 true 2726 true D1 DIRECT true 5810131 true /api/publication/2974496 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10052149"><a href="/gui2/?type=authors&mode=browse&sel=10052149" target="_blank">Farkas T (<span class="authorship-author-name">Farkas Tibor</span> <span class="authorAux-mtmt"> Biológia</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Szekely E </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Belak S </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10009849"><a href="/gui2/?type=authors&mode=browse&sel=10009849" target="_blank">Kiss I (<span class="authorship-author-name">Kiss István</span> <span class="authorAux-mtmt"> Állatorvosi laboratóriumi diagnosztika, diagnos...</span>) </a> </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;2974496" target="_blank">Real-time PCR-based pathotyping of Newcastle disease virus by use of TaqMan minor groove binder probes.</a></div> <div> <span class="journal-title">JOURNAL OF CLINICAL MICROBIOLOGY</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0095-1137">0095-1137</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1098-660X">1098-660X</a>)</span>: <span class="journal-volume">47</span> <span class="journal-issue">7</span> <span class="page"> pp 2114-2123 </span> <span class="year">(2009)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.1128/JCM.01652-08" target="_blank" href="https://doi.org/10.1128/JCM.01652-08"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000267713000018" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000267713000018"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="67650021215" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-67650021215"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="19439542" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=19439542&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Microbiology (medical)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 35</span> | Független: 33 | Függő: 2 | Nem jelölt: 0 | WoS jelölt: 35 | Scopus jelölt:&nbsp;17 | WoS/Scopus jelölt:&nbsp;35 | DOI jelölt:&nbsp;33 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;2974496&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 8 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 2974496</span> | <span class="status-data status-VALIDATED"> Egyeztetett </span> <span class="oldId">Régi azonosító: 2974496</span> | Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">MEDLINE</span> </div> <div class="lastModified">Utolsó módosítás: 2025.09.20. 12:56 Csernyik Rita (MTMT központi admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: : 10.1016/j.ijfoodmicro.2011.07.011. Epub 2011 Aug 4. PMC PMC2708511 Hiányzó Besorolás: 'Evaluation Studies\n\nJournal Article\n\nResearch Support, Non-U.S. Gov\'t',24 Megjegyzés-23107078 N1 Molecular Sequence Numbers: GENBANK: AF217084, AF309418, AJ629062, AJ880277, AY562991, DQ097394, EF201805, EU604247, EU604248, EU604249, EU604250, EU604251, EU604252, EU604253, EU604254, EU604255, EU604256...</pre> </div></div>