Appearance of fast astrocytic component in voltage-sensitive dye imaging of neural activity.

Pal, I [Pál, Ildikó (Kémia), szerző] Molekuláris Idegtudományi Laboratórium (MTA TTK / SZKI / FFCs); Kardos, J [Kardos, Julianna (Neurokémia, az id...), szerző] Szerves Kémiai Intézet (MTA TTK); Dobolyi, A [Dobolyi, Árpád (Idegtudomány), szerző] MTA-ELTE-NAP B Molekuláris és Rendszer Neurobio... (ELTE / TTK / Bio_I); Heja, L [Héja, László (Neurokémia), szerző] Szerves Kémiai Intézet (MTA TTK)

Angol nyelvű Tudományos Szakcikk (Folyóiratcikk)
Megjelent: MOLECULAR BRAIN 1756-6606 8 (1) Paper: 35 , 20 p. 2015
  • SJR Scopus - Cellular and Molecular Neuroscience: Q1
    BACKGROUND: Voltage-sensitive dye (VSD) imaging and intrinsic optical signals (IOS) are widely used methods for monitoring spatiotemporal neural activity in extensive networks. In spite of that, identification of their major cellular and molecular components has not been concluded so far. RESULTS: We addressed these issues by imaging spatiotemporal spreading of IOS and VSD transients initiated by Schaffer collateral stimulation in rat hippocampal slices with temporal resolution comparable to standard field potential recordings using a 464-element photodiode array. By exploring the potential neuronal and astroglial molecular players in VSD and IOS generation, we identified multiple astrocytic mechanisms that significantly contribute to the VSD signal, in addition to the expected neuronal targets. Glutamate clearance through the astroglial glutamate transporter EAAT2 has been shown to be a significant player in VSD generation within a very short (<5 ms) time-scale, indicating that astrocytes do contribute to the development of spatiotemporal VSD transients previously thought to be essentially neuronal. In addition, non-specific anion channels, astroglial K(+) clearance through Kir4.1 channel and astroglial Na(+)/K(+) ATPase also contribute to IOS and VSD transients. CONCLUSION: VSD imaging cannot be considered as a spatially extended field potential measurement with predominantly neuronal origin, instead it also reflects a fast communication between neurons and astrocytes.
    Hivatkozás stílusok: IEEEACMAPAChicagoHarvardCSLMásolásNyomtatás
    2020-07-07 08:53