Modeling of in vivo acousto-optic two-photon imaging of the retina in the human eye.

Kusnyerik, A [Kusnyerik, Ákos (Szemészet), author] Department of Ophthalmology (SU / FM / C); Rozsa, B [Rózsa J., Balázs (Idegtudomány, fizika), author] Two-photon Imaging Center (IEM / DP); Veress, M; Szabo, A [Szabó, Arnold (retina), author] Department of Human Morphology and Developmenta... (SU / FM / I); Nemeth, J [Németh, János Tibor (Szemészet), author] Department of Ophthalmology (SU / FM / C); Maak, P [Maák, Pál (Optika, lézerfizika), author] Department of Atomic Physics (BUTE / FNS / IP)

English Article (Journal Article) Scientific
Published: OPTICS EXPRESS 1094-4087 23 (18) pp. 23436-23449 2015
  • SJR Scopus - Atomic and Molecular Physics, and Optics: D1
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Subjects:
  • Physical sciences
Our aim is to establish a novel combined acousto-optical method for in vivo imaging of the human retina with the two-photon microscope. In this paper we present modeling results based on eye model samples constructed with parameters measured on patients. We used effectively the potential of the electronic compensation offered by the acousto-optic lenses to avoid the use of adaptive optical correction. Simulation predicted lateral resolution between 1.6 microm and 3 microm on the retina. This technology allows the visualization of single cells and promises real time measuring of neural activity in individual neurons, neural segments and cell assemblies with 30-100 micros temporal and subcellular spatial resolution.
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2026-10-15 01:25