A complete SGP null mutant (Sgp‐A1B1D1 null) winter wheat
line was crossed with three wheat varieties: ‘Ukrainka’, ‘Lona’ and ‘Solstice’. Three
backcrosses (BCs) were carried out and marker assisted selection used to identify
lines carrying all the three mutant alleles for each cross. BC3
and BC2xBC2 F3, F4
and F5 lines (2012‐2014) were studied in detail for physical
(thousand kernel weight), compositional (protein, gluten, amylose) and starch viscosity
properties (rapid visco analyser‐RVA). Ten triple mutant high‐amylose lines (∼40%)
were identified among the BC2xBC2 lines,
which had high protein content (>14%), low starch content, gluten index, starch viscosity
values and thousand kernel weight. Two of these lines had moderately high amylose
(∼30%) content, with outstanding gluten quality, but modified starch viscosity properties.
