Monitoring of Short-Term Erythropoietin Therapy in Rats with Acute Spinal Cord Injury Using Manganese-Enhanced Magnetic Resonance Imaging

Freitag, MT; Marton, G; Pajer, K [Pajer, Krisztián (idegtudomány), szerző] Anatómiai, Szövet- és Fejlődéstani Intézet (SZTE / SZAOK); Hartmann, J; Walder, N; Rossmann, M; Parzer, P; Redl, H; Nogradi, A [Nógrádi, Antal (idegtudomány, sze...), szerző] Anatómiai, Szövet- és Fejlődéstani Intézet (SZTE / SZAOK); Stieltjes, B ✉

Angol nyelvű Tudományos Szakcikk (Folyóiratcikk)
Megjelent: JOURNAL OF NEUROIMAGING 1051-2284 1552-6569 25 (4) pp. 582-589 2015
  • SJR Scopus - Medicine (miscellaneous): Q2
Azonosítók
Szakterületek:
    BACKGROUND AND PURPOSE: To evaluate the short-term outcome of erythropoietin (EPO) therapy in rats with spinal cord injury (SCI) using manganese-enhanced magnetic resonance imaging (MEMRI). METHODS: Rats were divided in an EPO and a control group. Laminectomy at Th11 was performed, followed by SCI. MnCl2 was applied into the cisterna magna and functional recovery was examined after injury using BBB-scoring. Then, rats were euthanized and the spinal cord was extracted for MEMRI. Finally, histological analysis was performed and correlated with MEMRI. RESULTS: EPO-treated animals showed significantly better functional recovery (P = .008, r = .62) and higher mean signal-to-noise ratio (SNR) in MEMRI compared to controls for slices 10-13 (P = .017, R2 = .31) at the level of the lesion epicenter. Functional recovery correlated significantly with higher SNR values, determined using the mean SNR between slices 10 and 13 (P = .047, R2 = .36). In this region, histology revealed a significantly decreased number of microglia cells and apoptosis in EPO-treated animals. CONCLUSION: MEMRI successfully depicts the therapeutic effect of EPO in early SCI that leads to a significant recovery in rats, a significantly reduced immune response and significantly reduced number of apoptotic cells at the height of the lesion epicenter.
    Hivatkozás stílusok: IEEEACMAPAChicagoHarvardCSLMásolásNyomtatás
    2022-01-25 03:44