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Dynamic light scattering (DLS) was used to monitor the coil-globule transition of calf thymus and bacterial DNAs by poly(ethylene glycol) (PEG 600 and PEG 6000) in aqueous NaCl solutions. The contribution of PEG 600 and PEG 6000 to DNA coil diameter change was investigated. Compaction of DNA molecules was observed for a PEG 6000 concentration ranging from 5.5 to 8%. Hydrophilic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) - P(HEMA-co-GMA), poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) - P(HEMA-co-EDMA), and poly(glycidyl methacrylate) (PGMA) microspheres containing carboxyl groups were used for DNA isolation. The highest DNA yield was achieved using 16% PEG 6000 and 2.0 M NaCl. The amount of isolated DNA correlated with the content of carboxyl groups on the microsphere surface. RNA adsorption on the surface of the microspheres tested was also studied. RNA recovery was more than one order lower than DNA recovery under the same conditions. All types of microspheres were tested for DNA isolation from crude cell lysates of different dairy products in solutions of 16% PEG 6000 and 2.0 M NaCl. Isolated DNAs were without PCR inhibitors, which was demonstrated using real-time PCR including melting analysis of the amplicons. © 2014 The Institution of Chemical Engineers.
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(Tóth Judit (Részecsketechnológia, p...); 2015.01.08. 09:43:30)
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<div class="JournalArticle Publication short-list"> <div class="authors"> <span class="author-name" > Trachtová, S </span> <span class="author-type"> </span> ; <span class="author-name" > Španová, A </span> <span class="author-type"> </span> ; <span class="author-name" mtid="10014679"> <a href="/gui2/?type=authors&mode=browse&sel=10014679" target="_blank">Tóth, J</a> </span> <span class="author-type"> </span> ; <span class="author-name" > Prettl, Z </span> <span class="author-type"> </span> ; <span class="author-name" > Horák, D </span> <span class="author-type"> </span> ; <span class="author-name" mtid="10001631"> <a href="/gui2/?type=authors&mode=browse&sel=10001631" target="_blank">Gyenis, J</a> </span> <span class="author-type"> </span> ; <span class="author-name" > Rittich, B </span> <span class="author-type"> </span> </div ><div class="title"><a href="/gui2/?mode=browse¶ms=publication;2806497" mtid="2806497" target="_blank">Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres</a></div> <div class="pub-info"> <span class="journal-title">FOOD AND BIOPRODUCTS PROCESSING</span> <span class="journal-volume">94</span> <span class="page"> pp. 375-81. </span> <span class="year">(2015)</span> </div> <div class="pub-end"><div class="identifier-list"> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:black" title="10.1016/j.fbp.2014.05.001" target="_blank" href="https://doi.org/10.1016/j.fbp.2014.05.001"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="84902072310" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84902072310"> Scopus </a> </span> </span> </div> <div class="short-pub-prop-list"> <span class="short-pub-mtid"> Közlemény:2806497 </span> <span class="status-holder"><span class="status-data status-APPROVED"> Nyilvános </span></span> <span class="pub-core">Forrás </span> <span class="pub-type">Folyóiratcikk (Szakcikk ) </span> <!-- && !record.category.scientific --> <span class="pub-category">Tudományos</span> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 12</span> | Független: 9 | Függő: 3 | Nem jelölt: 0 | WoS jelölt: 12 | Scopus jelölt: 12 | WoS/Scopus jelölt: 12 | DOI jelölt: 12 </div> </div> </div> </div><div class="JournalArticle Publication long-list">
<div class="authors">
<img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png">
<div class="autype autype0"> <span class="author-name" >Trachtová S
</span>
;
<span class="author-name" >Španová A
</span>
;
<span class="author-name" mtid="10014679"><a
href="/gui2/?type=authors&mode=browse&sel=10014679" target="_blank">Tóth J
(<span class="authorship-author-name">Tóth Judit</span>
<span class="authorAux-mtmt"> Részecsketechnológia, plazmakémia</span>)
</a>
</span>
<span class="author-affil"><span title="MTA Természettudományi Kutatóközpont">MTA TTK</span>/<span title="Anyag- és Környezetkémiai Intézet">AKI</span>/Funkcionális Nanorészecskék Csoport</span>
;
<span class="author-name" >Prettl Z
</span>
;
<span class="author-name" >Horák D
</span>
;
<span class="author-name" mtid="10001631"><a
href="/gui2/?type=authors&mode=browse&sel=10001631" target="_blank">Gyenis J
(<span class="authorship-author-name">Gyenis János</span>
<span class="authorAux-mtmt"> Vegyipari műveletek, eljárások</span>)
</a>
</span>
<span class="author-affil"><span title="Pannon Egyetem">PE</span>/<span title="Műszaki Informatikai Kar">MIK</span>/Műszaki Kémiai Kutatóintézet</span>
;
<span class="author-name" >Rittich B
</span>
</div>
</div>
<div class="title"><a href="/gui2/?mode=browse¶ms=publication;2806497" target="_blank">Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres</a></div> <div> <span class="journal-title">FOOD AND BIOPRODUCTS PROCESSING</span>
<span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0960-3085">0960-3085</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1744-3571">1744-3571</a>)</span>:
<span class="journal-volume">94</span>
<span class="page">
pp 375-81
</span> <span class="year">(2015)</span>
</div>
<div class="pub-footer">
<span class="language" xmlns="http://www.w3.org/1999/html">Nyelv:
Angol
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<span class="identifiers">
<span class="id identifier oa_GOLD" title=" Gold
">
<a style="color:black" title="10.1016/j.fbp.2014.05.001" target="_blank" href="https://doi.org/10.1016/j.fbp.2014.05.001">
DOI
</a>
</span>
<span class="id identifier oa_none" title="none">
<a style="color:black" title="84902072310" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84902072310">
Scopus
</a>
</span>
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<div class="publication-citation" style="margin-left: 0.5cm;">
<span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 12</span>
| Független: 9
| Függő: 3
| Nem jelölt: 0
| WoS jelölt: 12
| Scopus jelölt: 12
| WoS/Scopus jelölt: 12
| DOI jelölt: 12
</div>
<div class="mtid"><span class="long-pub-mtid">Közlemény: 2806497</span>
| <span class="status-data status-APPROVED"> Nyilvános
</span>
<span class="oldId">Régi azonosító: 2806497</span> |
Forrás
| <span class="type-subtype">Folyóiratcikk
( Szakcikk
)
</span>
| <span class="pub-category">Tudományos</span>
| <span class="publication-sourceOfData">Scopus</span>
</div>
<div class="lastModified">Utolsó módosítás: 2023.09.19. 17:48 MTMT API (MTMT API user, admin)
</div>
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