https://m2.mtmt.hu/ eng 2026-10-25 14:55 JournalArticle 2774695 APPROVED true Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine Hungarian Academy of Sciences, Budapest, 1083, Hungary Department of Anatomy, Hokkaido University School of Medicine, Sapporo, 060-8638, Japan János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, 1085, Hungary Cited By :38 Export Date: 28 January 2025 Correspondence Address: Nyiri, G.; Laboratory of Cerebral Cortex Research, Hungary; email: nyiri.gabor@koki.mta.hu Chemicals/CAS: Glutamates; Receptors, N-Methyl-D-Aspartate; Vesicular Glutamate Transport Proteins Funding details: CNK77793, K83251 Funding details: National Institutes of Health, NIH Funding details: National Institute of Neurological Disorders and Stroke, NINDS, R37NS030549 Funding details: European Commission, EC Funding details: European Research Council, ERC, ERC-2011-ADG-294313 Funding details: European Social Fund, ESF, A/1-11-1-2012-0001 Funding text 1: We thank Em?ke Szépné Simon, Katalin Lengyel, Katalin Iványi and Gy?z? Goda for the excellent technical assistance and Dr. Ferenc Mátyás for technical discussions. We thank Dr. Viktor Varga for his comments on the previous version of the manuscript. The authors wish to thank László Barna, the Nikon Microscopy Center at IEM, Nikon Austria GmbH and Auro-Science Consulting Ltd for kindly providing technical support for fluorescent microscopy. This work was supported by the National Institutes of Health (grant number NS030549), National Office for Research and TechnologyâHungarian Scientific Research Fund (NKTH-OTKA, grant number CNK77793, K83251) and European Research Council (grant number ERC-2011-ADG-294313, SERRACO). András Sz?nyi was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TÃMOP 4.2.4. A/1-11-1-2012-0001 "National Excellence Program". Gabor Nyiri was supported by a János Bolyai Research Scholarship. 0 2018-10-02T08:54:25.000+0000 false 2774695 2026-10-16T21:16:21.354+0000 2024-09-17T09:45:27.765+0000 2014-11-17T06:50:08.000+0000 true 10012277 Nyiri Gábor /api/author/10012277 Author true 10012277 2025-11-12T09:04:03.228+0000 2025-11-12T09:04:03.228+0000 2020-12-16T14:29:03.963+0000 true 10062250 Molnár-Taga Márta /api/admin/10062250 Admin true 10062250 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Szonyi, A The ascending median raphe projections are mainly glutamatergic in the mouse forebrain. true true /api/journal/10006324 REVIEWED true false 10006324 false 10006324 true 1863-2653 1863-2661 Journal FOREIGN 221 2 735 751 735 17 2016 The median raphe region (MRR) is thought to be serotonergic and plays an important role in the regulation of many cognitive functions. In the hippocampus (HIPP), the MRR exerts a fast excitatory control, partially through glutamatergic transmission, on a subpopulation of GABAergic interneurons that are key regulators of local network activity. However, not all receptors of this connection in the HIPP and in synapses established by MRR in other brain areas are known. Using combined anterograde tracing and immunogold methods, we show that the GluN2A subunit of the NMDA receptor is present in the synapses established by MRR not only in the HIPP, but also in the medial septum (MS) and in the medial prefrontal cortex (mPFC) of the mouse. We estimated similar amounts of NMDA receptors in these synapses established by the MRR and in local adjacent excitatory synapses. Using retrograde tracing and confocal laser scanning microscopy, we found that the majority of the projecting cells of the mouse MRR contain the vesicular glutamate transporter type 3 (vGluT3). Furthermore, using double retrograde tracing, we found that single cells of the MRR can innervate the HIPP and mPFC or the MS and mPFC simultaneously, and these double-projecting cells are also predominantly vGluT3-positive. Our results indicate that the majority of the output of the MRR is glutamatergic and acts through NMDA receptor-containing synapses. This suggests that key forebrain areas receive precisely targeted excitatory input from the MRR, which is able to synchronously modify activity in those regions via individual MRR cells with dual projections. 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<span title="Semmelweis University">SU</span>/School of Ph. D. Studies</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10062895"><a href="/gui2/?type=authors&mode=browse&sel=10062895" target="_blank">Mayer MI (<span class="authorship-author-name">Mayer Márton István</span> <span class="authorAux-mtmt"> Neurobiológia</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Cellular and Network Neurobiology">DCNN</span>/Laboratory of Cerebral Cortex Research</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="172"><a href="/gui2/?type=authors&mode=browse&sel=172" target="_blank">Cserep C (<span class="authorship-author-name">Cserép Csaba</span> <span class="authorAux-mtmt"> Idegtudomány, Neurobiológia</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Cellular and Network Neurobiology">DCNN</span>/Laboratory of Cerebral Cortex Research</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="223"><a href="/gui2/?type=authors&mode=browse&sel=223" target="_blank">Takacs VT (<span class="authorship-author-name">Tresóné Takács Virág</span> <span class="authorAux-mtmt"> Neurobiológia</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Cellular and Network Neurobiology">DCNN</span>/Laboratory of Cerebral Cortex Research</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Watanabe M </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000275"><a href="/gui2/?type=authors&mode=browse&sel=10000275" target="_blank">Freund TF (<span class="authorship-author-name">Freund Tamás</span> <span class="authorAux-mtmt"> Neurobiológia, idegtudomány</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Cellular and Network Neurobiology">DCNN</span>/Laboratory of Cerebral Cortex Research</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10012277"><a href="/gui2/?type=authors&mode=browse&sel=10012277" target="_blank">Nyiri G ✉ (<span class="authorship-author-name">Nyiri Gábor</span> <span class="authorAux-mtmt"> Neurobiológia</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Cellular and Network Neurobiology">DCNN</span>/Laboratory of Cerebral Cortex Research</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;2774695" target="_blank">The ascending median raphe projections are mainly glutamatergic in the mouse forebrain.</a></div> <div> <span class="journal-title">BRAIN STRUCTURE & FUNCTION</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1863-2653">1863-2653</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1863-2661">1863-2661</a>)</span>: <span class="journal-volume">221</span> <span class="journal-issue">2</span> <span class="page"> pp 735-751 </span> <span class="year">(2016)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_PAY" title=" Pay "> <a style="color:blue" title="10.1007/s00429-014-0935-1" target="_blank" href="https://doi.org/10.1007/s00429-014-0935-1"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000371380100003" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000371380100003"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="115112" target="_blank" href="https://real.mtak.hu/115112"> REAL </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="84959330257" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84959330257"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="25381463" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=25381463&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Anatomy&nbsp;&nbsp;&nbsp;Rank:&nbsp;D1</div> <div class="journal-subject">Journal subject: Scopus - Histology&nbsp;&nbsp;&nbsp;Rank:&nbsp;D1</div> <div class="journal-subject">Journal subject: Scopus - Neuroscience (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 43</span> | Independent citation: 39 | Self citation: 4 | Unknown citation: 0 | Number of citations in WoS: 42 | Number of citations in Scopus:&nbsp;41 | WoS/Scopus assigned:&nbsp;43 | Number of citations with DOI:&nbsp;43 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;2774695&sort=publishedYear,desc&sort=title"> Number of cited publications: 12 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 2774695</span> | <span class="status-data status-APPROVED"> Published </span> <span class="oldId">Old id: 2774695</span> | Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">MEDLINE</span> </div> <div class="funder"> NINDS(R37NS030549), Seventh Framework Programme(FP7_294313) </div> <div class="lastModified">Last Modified: 2024.09.17. 11:45 Annamária Boros (MTMT Központi admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Comments</u>: Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine Hungarian Academy of Sciences, Budapest, 1083, Hungary Department of Anatomy, Hokkaido University School of Medicine, Sapporo, 060-8638, Japan János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, 1085, Hungary Cited B...</pre> </div></div>