Mesenchymal stems or stromal cells (MSCs) are rare multipotent cells with potent regenerative
and immunomodulatory properties. Microglial cells (MGs) are specialized tissue macrophages
of the central nervous system (CNS) that continuously survey their environment with
highly motile extensions. Recently, several studies have shown that MSCs are capable
of reprogramming microglia into an "M2-like" phenotype characterized by increased
phagocytic activity and upregulated expression of anti-inflammatory mediators in vitro.
However, the precise polarization states of microglia in the presence of MSCs under
physiological or under inflammatory conditions remain largely unknown. In this study,
we found that MSCs induce a mixed microglia phenotype defined as Arg1-high, CD86-high,
CD206-high, IL-10-high, PGE2-high, MCP-1/CCL2-high, IL-1beta-moderate, NALP-3-low,
and TNF-alpha-low cells. These MSC-elicited MGs have high phagocytic activity and
antigen-presenting ability. Lipopolysaccharide is able to shape this microglia phenotype
quantitatively, but not qualitatively in the presence of MSCs. This unique polarization
state resembles a novel regulatory microglia phenotype, which might contribute to
the resolution of inflammation and to tissue repair in the CNS.
