https://m2.mtmt.hu/ eng 2026-10-24 02:14 JournalArticle 2739697 APPROVED true Cited By :15 Export Date: 28 January 2025 CODEN: JNRSD Correspondence Address: Nusser, Z.; Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Szigony str. 43, Budapest 1083, Hungary; email: nusser@koki.hu Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; 4 ethyl 6,7 dimethoxy beta carboline 3 carboxylic acid methyl ester, 82499-00-1; biocytin, 576-19-2; deoxycorticosterone, 64-85-7; flurazepam, 1172-18-5, 17617-23-1; lysine, 56-87-1, 6899-06-5, 70-54-2; tetrahydrodeoxycorticosterone, 567-03-3 0 2015-02-17T23:20:46.000+0000 false 2739697 2026-10-16T19:29:32.602+0000 2024-09-16T14:58:55.236+0000 2014-09-23T15:19:51.000+0000 true 10000499 Nusser Zoltán /api/author/10000499 Author true 10000499 2025-03-17T08:33:20.369+0000 2025-03-17T08:33:20.369+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Kerti-Szigeti, K Synaptic GABAA receptor clustering without the gamma2 subunit. true true /api/journal/2707 REVIEWED true false 2707 false 2707 true 0270-6474 1529-2401 Journal FOREIGN 34 31 10219 10233 10219 15 2014 Rapid activation of postsynaptic GABAA receptors (GABAARs) is crucial in many neuronal functions, including the synchronization of neuronal ensembles and controlling the precise timing of action potentials. Although the gamma2 subunit is believed to be essential for the postsynaptic clustering of GABAARs, synaptic currents have been detected in neurons obtained from gamma2(-/-) mice. To determine the role of the gamma2 subunit in synaptic GABAAR enrichment, we performed a spatially and temporally controlled gamma2 subunit deletion by injecting Cre-expressing viral vectors into the neocortex of GABAARgamma2(77I)lox mice. Whole-cell recordings revealed the presence of miniature IPSCs in Cre(+) layer 2/3 pyramidal cells (PCs) with unchanged amplitudes and rise times, but significantly prolonged decays. Such slowly decaying currents could be evoked in PCs by action potentials in presynaptic fast-spiking interneurons. Freeze-fracture replica immunogold labeling revealed the presence of the alpha1 and beta3 subunits in perisomatic synapses of cells that lack the gamma2 subunit. Miniature IPSCs in Cre(+) PCs were insensitive to low concentrations of flurazepam, providing a pharmacological confirmation of the lack of the gamma2 subunit. Receptors assembled from only alphabeta subunits were unlikely because Zn(2+) did not block the synaptic currents. Pharmacological experiments indicated that the alphabetagamma3 receptor, rather than the alphabetadelta, alphabetaepsilon, or alphabetagamma1 receptors, was responsible for the slowly decaying IPSCs. Our data demonstrate the presence of IPSCs and the synaptic enrichment of the alpha1 and beta3 subunits and suggest that the gamma3 subunit is the most likely candidate for clustering GABAARs at synapses in the absence of the gamma2 subunit. true true 2014 803170 true true true false true false GOLD 2026-10-16 true https://doi.org/10.1523/JNEUROSCI.1721-14.2014 16 0 16 15 1 16 16 14 16 16 16 15 15 15 1 0 16 15 16 15 0 10 10 Folyóiratcikk Journal Article 24 15 Könyvrészlet Chapter in Book 25 1 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2015 0 1 1 1 1 2016 0 4 3 4 3 2017 0 2 2 2 2 2018 0 3 3 3 3 2019 0 1 1 1 1 2020 0 1 1 1 1 2021 0 1 1 1 1 2022 0 0 0 0 0 2023 0 0 0 0 0 2024 0 1 1 1 1 2025 0 1 1 1 1 2026 0 1 1 1 1 D1 false false false false 2015-02-01T13:44:16.000+0000 2014-11-03T13:44:16.000+0000 Celluláris Idegélettani Kutatócsoport (HRN KOKI) 3 10 1 3 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 5484552 /api/authorship/5484552 1 0.33333334 true false Author 210 /api/author/210 Kerti-Szigeti Katalin true 210 true Kerti-Szigeti K true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 20289471 true PersonAuthorship 5484553 /api/authorship/5484553 2 0.333 false false true Author 10000499 /api/author/10000499 Nusser Zoltán true 10000499 true Nusser Z true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 20289470 true PersonAuthorship 5484554 /api/authorship/5484554 3 0.333 false true Author 10012932 /api/author/10012932 Eyre Mark David true 10012932 true Eyre MD true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 20289469 true PublicationIdentifier 893013 /api/publicationidentifier/893013 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD false IDENTICAL 10.1523/JNEUROSCI.1721-14.2014 https://doi.org/10.1523/JNEUROSCI.1721-14.2014 false 954595 true PublicationIdentifier 15033144 /api/publicationidentifier/15033144 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true IDENTICAL 000341009500008 https://www.webofscience.com/wos/woscc/full-record/000341009500008 false 12053069 true PublicationIdentifier 15033145 /api/publicationidentifier/15033145 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true IDENTICAL 84905027738 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84905027738 false 15457378 true PublicationIdentifier 893014 /api/publicationidentifier/893014 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true false NO 25080584 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=25080584&dopt=Abstract false 954596 true SjrRating 11097833 /api/sjrrating/11097833 11 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 2801 /api/classificationexternal/2801 true 2801 true D1 DIRECT true true /api/publication/2739697 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="210"><a href="/gui2/?type=authors&mode=browse&sel=210" target="_blank">Kerti-Szigeti K (<span class="authorship-author-name">Kerti-Szigeti Katalin</span> <span class="authorAux-mtmt"> Celluláris idegélettan</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Cellular and Network Neurobiology">DCNN</span>/Laboratory of Cellular Neurophysiology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000499"><a href="/gui2/?type=authors&mode=browse&sel=10000499" target="_blank">Nusser Z ✉ (<span class="authorship-author-name">Nusser Zoltán</span> <span class="authorAux-mtmt"> Celluláris idegélettan</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Cellular and Network Neurobiology">DCNN</span>/Laboratory of Cellular Neurophysiology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10012932"><a href="/gui2/?type=authors&mode=browse&sel=10012932" target="_blank">Eyre MD (<span class="authorship-author-name">Eyre Mark David</span> <span class="authorAux-mtmt"> Neuroanatómia, idegélettan</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Cellular and Network Neurobiology">DCNN</span>/Laboratory of Cellular Neurophysiology</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;2739697" target="_blank">Synaptic GABAA receptor clustering without the gamma2 subunit.</a></div> <div> <span class="journal-title">JOURNAL OF NEUROSCIENCE</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0270-6474">0270-6474</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1529-2401">1529-2401</a>)</span>: <span class="journal-volume">34</span> <span class="journal-issue">31</span> <span class="page"> pp 10219-10233 </span> <span class="year">(2014)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:blue" title="10.1523/JNEUROSCI.1721-14.2014" target="_blank" href="https://doi.org/10.1523/JNEUROSCI.1721-14.2014"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000341009500008" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000341009500008"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="84905027738" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84905027738"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="25080584" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=25080584&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Neuroscience (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;D1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 16</span> | Independent citation: 15 | Self citation: 1 | Unknown citation: 0 | Number of citations in WoS: 14 | Number of citations in Scopus:&nbsp;16 | WoS/Scopus assigned:&nbsp;16 | Number of citations with DOI:&nbsp;16 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;2739697&sort=publishedYear,desc&sort=title"> Number of cited publications: 10 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 2739697</span> | <span class="status-data status-APPROVED"> Published </span> <span class="oldId">Old id: 2739697</span> | Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">MEDLINE</span> </div> <div class="lastModified">Last Modified: 2024.09.16. 16:58 Annamária Boros (MTMT Központi admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Comments</u>: Cited By :15 Export Date: 28 January 2025 CODEN: JNRSD Correspondence Address: Nusser, Z.; Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Szigony str. 43, Budapest 1083, Hungary; email: nusser@koki.hu Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; 4 ethyl 6,7 di...</pre> </div></div>