Phasic, nonsynaptic GABA-A receptor-mediated inhibition entrains thalamocortical oscillations.

Rovo, Z [Rovó, Zita (Neurobiológia), author] Laboratory of Thalamus Research; Matyas, F* [Mátyás, Ferenc (Neurobiológia), author] Laboratory of Thalamus Research; Bartho, P [Barthó, Péter (Idegélettan), author] Laboratory of Thalamus Research; Slezia, A [Slézia, Andrea (Idegtudomány), author] Laboratory of Thalamus Research; Lecci, S; Pellegrini, C; Astori, S; David, C [Dávid, Csaba (idegrendszer), author] Laboratory of Thalamus Research; Department of Human Morphology and Developmenta... (SU / FM / I); Hangya, B [Hangya, Balázs (Neurobiológia), author] Laboratory of Cerebral Cortex Research (IEM / DCNN); Luthi, A; Acsady, L ✉ [Acsády, László (Neurobiológia), author] Laboratory of Thalamus Research

English Article (Journal Article) Scientific
Published: JOURNAL OF NEUROSCIENCE 0270-6474 1529-2401 34 (21) pp. 7137-7147 2014
  • SJR Scopus - Neuroscience (miscellaneous): D1
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  • Basic medicine
GABA-A receptors (GABA-ARs) are typically expressed at synaptic or nonsynaptic sites mediating phasic and tonic inhibition, respectively. These two forms of inhibition conjointly control various network oscillations. To disentangle their roles in thalamocortical rhythms, we focally deleted synaptic, gamma2 subunit-containing GABA-ARs in the thalamus using viral intervention in mice. After successful removal of gamma2 subunit clusters, spontaneous and evoked GABAergic synaptic currents disappeared in thalamocortical cells when the presynaptic, reticular thalamic (nRT) neurons fired in tonic mode. However, when nRT cells fired in burst mode, slow phasic GABA-AR-mediated events persisted, indicating a dynamic, burst-specific recruitment of nonsynaptic GABA-ARs. In vivo, removal of synaptic GABA-ARs reduced the firing of individual thalamocortical cells but did not abolish slow oscillations or sleep spindles. We conclude that nonsynaptic GABA-ARs are recruited in a phasic manner specifically during burst firing of nRT cells and provide sufficient GABA-AR activation to control major thalamocortical oscillations.
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2024-05-29 23:47