https://m2.mtmt.hu/ eng 2026-10-23 00:39 JournalArticle 2714460 APPROVED true Cited By :8 Export Date: 28 February 2025 CODEN: JMLEE Correspondence Address: Gereben, B.; Department of Endocrine Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony Street 43, Hungary Chemicals/CAS: lysine, 56-87-1, 6899-06-5, 70-54-2; thyroxine deiodinase, 70712-46-8; ubiquitin, 60267-61-0; iodide peroxidase, 9031-28-1; Iodide Peroxidase; Recombinant Fusion Proteins; Thyroid Hormones 0 2018-02-28T20:07:12.000+0000 false 2714460 2026-10-16T18:01:56.122+0000 2025-02-27T14:00:08.149+0000 2014-08-01T07:12:23.000+0000 true 10011473 Gereben Balázs /api/author/10011473 Author true 10011473 2025-11-11T08:58:37.803+0000 2025-11-11T08:58:37.803+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Egri, P Minimal requirements for ubiquitination mediated regulation of thyroid hormone activation. true true /api/journal/2671 REVIEWED true false 2671 false 2671 true 0952-5041 1479-6813 Journal FOREIGN 53 2 217 226 217 10 2014 Activation of thyroxine by outer ring deiodination is the crucial first step of thyroid hormone action. Substrate-induced ubiquitination of type 2 deiodinase (D2) is the most rapid and sensitive mechanism known to regulate thyroid hormone activation. While the molecular machinery responsible for D2 ubiquitination has been extensively studied, the combination of molecular features sufficient and required to allow D2 ubiquitination remained to be determined. To address this question we constructed chimeric deiodinases by introducing different combinations of D2-specific elements into type 1 deiodinase (D1), another member of the deiodinase enzyme family, which however does not undergo ubiquitination in its native form. Studies on the chimeric proteins expressed transiently in HEK-293T cells revealed that combined insertion of the D2-specific instability loop and the K237/K244 D2 ubiquitin-carrier lysines into the corresponding positions of D1 could-not ubiquitinate D1 unless the chimera was directed to the endoplasmic reticulum (ER). Fluorescence resonance energy transfer measurements demonstrated that the C-terminal globular domain of the ER-directed chimera was able to interact with the E3 ligase subunit WSB1. However, this interaction did not occur between the chimera and the TEB4 E3 ligase although a native D2 could readily interact with the N-terminus of TEB4. In conclusion, insertion of the instability loop and ubiquitin-carrier lysines in combination with direction to the ER are sufficient and required to govern WSB1-mediated ubiquitination of an activating deiodinase enzyme. 2014 797441 true true true false true false GREEN GREEN 2026-10-16 true https://real.mtak.hu/18190 true 10030885 Bálint Eszter /api/admin/10030885 Admin true 10030885 9 0 9 2 7 9 9 9 9 9 9 2 2 2 7 0 9 2 9 2 0 14 14 Folyóiratcikk Journal Article 24 9 Könyvrészlet Chapter in Book 25 0 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2015 0 1 0 1 0 2016 0 2 0 2 0 2017 0 1 1 1 1 2018 0 1 0 1 0 2019 0 2 0 2 0 2020 0 0 0 0 0 2021 0 1 1 1 1 2022 0 0 0 0 0 2023 0 0 0 0 0 2024 0 0 0 0 0 2025 0 1 0 1 0 Q1 false 206 false false false 2015-01-07T14:50:33.000+0000 2014-10-09T13:50:33.000+0000 Doktori Iskola (SE); Molekuláris Sejt Metabolizmus Kutatócsoport (HRN KOKI) 2 19 2 2 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 5402001 /api/authorship/5402001 1 0.5 true false Author 206 /api/author/206 Egri Péter true 206 true Egri P true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 20145308 true PersonAuthorship 5402002 /api/authorship/5402002 2 0.5 false true true Author 10011473 /api/author/10011473 Gereben Balázs true 10011473 true Gereben B true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 20145307 true PublicationIdentifier 878547 /api/publicationidentifier/878547 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true PAY false IDENTICAL 10.1530/JME-14-0156 https://doi.org/10.1530/JME-14-0156 false 919195 true PublicationIdentifier 878549 /api/publicationidentifier/878549 SwordSource 36 /api/publicationsource/36 PublicationSourceType 10007 /api/publicationsourcetype/10007 true true true REAL REAL https://real.mtak.hu/@@@ true true 36 true GREEN true NO 18190 https://real.mtak.hu/18190 false 985284 true PublicationIdentifier 878545 /api/publicationidentifier/878545 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true false IDENTICAL 000345620300012 https://www.webofscience.com/wos/woscc/full-record/000345620300012 false 1165810 true PublicationIdentifier 878546 /api/publicationidentifier/878546 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true false IDENTICAL 84908001580 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84908001580 false 1731459 true PublicationIdentifier 878548 /api/publicationidentifier/878548 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true false NO 25074266 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=25074266&dopt=Abstract false 919196 true Classification 10545 /api/classification/10545 true true Classification 12215 /api/classification/12215 true true SjrRating 11077267 /api/sjrrating/11077267 22 0.24 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 1310 /api/classificationexternal/1310 true 1310 true Q1 DIRECT true true /api/publication/2714460 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="206"><a href="/gui2/?type=authors&mode=browse&sel=206" target="_blank">Egri P (<span class="authorship-author-name">Egri Péter</span> <span class="authorAux-mtmt"> Endokrinológia</span>) </a> </span> <span class="author-affil"><span title="Semmelweis University">SU</span>/School of Ph. D. Studies; <span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Endocrine Neurobiology">DEN</span>/Laboratory of Molecular Cell Metabolism</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10011473"><a href="/gui2/?type=authors&mode=browse&sel=10011473" target="_blank">Gereben B ✉ (<span class="authorship-author-name">Gereben Balázs</span> <span class="authorAux-mtmt"> Pajzsmirigy endokrinológia</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Endocrine Neurobiology">DEN</span>/Laboratory of Molecular Cell Metabolism</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;2714460" target="_blank">Minimal requirements for ubiquitination mediated regulation of thyroid hormone activation.</a></div> <div> <span class="journal-title">JOURNAL OF MOLECULAR ENDOCRINOLOGY</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0952-5041">0952-5041</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1479-6813">1479-6813</a>)</span>: <span class="journal-volume">53</span> <span class="journal-issue">2</span> <span class="page"> pp 217-226 </span> <span class="year">(2014)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_PAY" title=" Pay "> <a style="color:blue" title="10.1530/JME-14-0156" target="_blank" href="https://doi.org/10.1530/JME-14-0156"> DOI </a> </span> <span class="id identifier oa_GREEN" title=" Green "> <a style="color:black" title="18190" target="_blank" href="https://real.mtak.hu/18190"> REAL </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000345620300012" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000345620300012"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="84908001580" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84908001580"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="25074266" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=25074266&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Endocrinology&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> <div class="journal-subject">Journal subject: Scopus - Molecular Biology&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 9</span> | Independent citation: 2 | Self citation: 7 | Unknown citation: 0 | Number of citations in WoS: 9 | Number of citations in Scopus:&nbsp;9 | WoS/Scopus assigned:&nbsp;9 | Number of citations with DOI:&nbsp;9 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;2714460&sort=publishedYear,desc&sort=title"> Number of cited publications: 14 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 2714460</span> | <span class="status-data status-APPROVED"> Published </span> <span class="oldId">Old id: 2714460</span> | Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">MEDLINE</span> </div> <div class="lastModified">Last Modified: 2025.02.27. 15:00 Márta Turek (KOKI admin 4) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Comments</u>: Cited By :8 Export Date: 28 February 2025 CODEN: JMLEE Correspondence Address: Gereben, B.; Department of Endocrine Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony Street 43, Hungary Chemicals/CAS: lysine, 56-87-1, 6899-06-5, 70-54-2; thyroxine deiodinase, 707...</pre> </div></div>