https://m2.mtmt.hu/ eng 2026-10-23 17:47 JournalArticle 2710791 APPROVED true Interdisciplinary Institute for Neuroscience (IINS), University of Bordeaux, Bordeaux, France UMR 5297, Centre National de la Recherche Scientifique (CNRS), Bordeaux, France Two-photon Imaging Center, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary Cited By :266 Export Date: 23 March 2023 CODEN: NANEF Correspondence Address: Nägerl, U.V.; Interdisciplinary Institute for Neuroscience (IINS), University of Bordeaux, Bordeaux, France; email: valentin.nagerl@u-bordeaux.fr Chemicals/CAS: glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4 Funding details: ANR-10-INSB-04 Funding details: 272351 Funding details: European Molecular Biology Organization, EMBO, 1518-2010 Funding details: Agence Nationale de la Recherche, ANR Funding details: Institut National de la Santé et de la Recherche Médicale, Inserm Funding text 1: We thank D. DiGregorio (Pasteur Institute) and all members of the Nägerl laboratory for comments on the manuscript, and J. Angibaud for excellent technical support. This work was supported by postdoctoral fellowships from the Marie-Curie Program (grant 272351) and the European Molecular Biology Organization (EMBO; grant 1518-2010) to J.T. and by grants from the French Institute of Health and Medical Research (INSERM), Agence Nationale de la Recherche (ANR), the Human Frontiers Science program (HFSP) and France-BioImaging (ANR-10-INSB-04) to U.V.N. 0 2015-12-17T12:35:02.000+0000 false 2710791 2026-10-16T17:50:58.150+0000 2023-03-21T13:04:43.949+0000 2014-07-22T05:21:29.000+0000 true 10012926 Rózsa J. Balázs /api/author/10012926 Author true 10012926 2025-03-22T18:51:35.124+0000 2025-03-22T18:51:35.124+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Tonnesen, J Spine neck plasticity regulates compartmentalization of synapses. true true /api/journal/19517 REVIEWED true false 19517 false 19517 true 1097-6256 1546-1726 Journal FOREIGN 17 5 678 685 678 8 2014 Dendritic spines have been proposed to transform synaptic signals through chemical and electrical compartmentalization. However, the quantitative contribution of spine morphology to synapse compartmentalization and its dynamic regulation are still poorly understood. We used time-lapse super-resolution stimulated emission depletion (STED) imaging in combination with fluorescence recovery after photobleaching (FRAP) measurements, two-photon glutamate uncaging, electrophysiology and simulations to investigate the dynamic link between nanoscale anatomy and compartmentalization in live spines of CA1 neurons in mouse brain slices. We report a diversity of spine morphologies that argues against common categorization schemes and establish a close link between compartmentalization and spine morphology, wherein spine neck width is the most critical morphological parameter. We demonstrate that spine necks are plastic structures that become wider and shorter after long-term potentiation. These morphological changes are predicted to lead to a substantial drop in spine head excitatory postsynaptic potential (EPSP) while preserving overall biochemical compartmentalization. true true 2014 789316 true true true false true false GREEN GREEN 2026-10-16 true https://real.mtak.hu/22358 375 0 372 344 8 364 363 342 349 371 371 343 343 347 28 0 375 347 372 344 0 3 3 Folyóiratcikk Journal Article 24 347 Könyvrészlet Chapter in Book 25 22 Könyv Book 23 2 Egyéb konferenciaközlemény Conference paper 31 1 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 3 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2014 0 9 8 9 8 2015 0 37 32 37 32 2016 0 51 48 51 48 2017 0 34 31 34 31 2018 0 33 29 33 29 2019 0 26 25 26 25 2020 0 25 22 25 22 2021 0 29 28 29 28 2022 0 29 28 29 28 2023 0 32 30 32 30 2024 0 29 28 29 28 2025 0 27 26 27 26 2026 0 14 12 14 12 D1 false false false false 2014-08-15T07:20:42.000+0000 2014-07-22T05:23:27.000+0000 Két-foton Képalkotó Központ (KOKI / GYO) 2 21 1 4 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 5394455 /api/authorship/5394455 1 0.25 true false Tonnesen J true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 20131567 true PersonAuthorship 5394456 /api/authorship/5394456 2 0.25 false false Author 191 /api/author/191 Katona Gergely true 191 true Katona G true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 20131566 true PersonAuthorship 5394457 /api/authorship/5394457 3 0.25 false false Author 10012926 /api/author/10012926 Rózsa J. Balázs true 10012926 true Rozsa B true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 20131565 true PersonAuthorship 5394458 /api/authorship/5394458 4 0.25 false true true Nagerl UV true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 20131564 true PublicationIdentifier 876350 /api/publicationidentifier/876350 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true PAY false IDENTICAL 10.1038/nn.3682 https://doi.org/10.1038/nn.3682 false 909906 true PublicationIdentifier 876352 /api/publicationidentifier/876352 SwordSource 36 /api/publicationsource/36 PublicationSourceType 10007 /api/publicationsourcetype/10007 true true true REAL REAL https://real.mtak.hu/@@@ true true 36 true GREEN true NO 22358 https://real.mtak.hu/22358 false 1094256 true PublicationIdentifier 876348 /api/publicationidentifier/876348 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true false IDENTICAL 000335016200011 https://www.webofscience.com/wos/woscc/full-record/000335016200011 false 1165846 true PublicationIdentifier 18717342 /api/publicationidentifier/18717342 PlainSource 111 /api/publicationsource/111 PublicationSourceType 10007 /api/publicationsourcetype/10007 true true true ResearchGate publ. https://www.researchgate.net/publication/@@@ true true 111 true 261033645 https://www.researchgate.net/publication/261033645 true true PublicationIdentifier 876349 /api/publicationidentifier/876349 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true false IDENTICAL 84899633952 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84899633952 false 1218585 true PublicationIdentifier 876351 /api/publicationidentifier/876351 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true false NO 24657968 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=24657968&dopt=Abstract false 909907 true Classification 12125 /api/classification/12125 true true Keyword 12 /api/keyword/12 true 12 true Keyword 629 /api/keyword/629 true 629 true Keyword 3441 /api/keyword/3441 true 3441 true Keyword 7884 /api/keyword/7884 true 7884 true Keyword 7927 /api/keyword/7927 true 7927 true Keyword 10282 /api/keyword/10282 true 10282 true Keyword 16571 /api/keyword/16571 true 16571 true Keyword 16672 /api/keyword/16672 true 16672 true Keyword 21259 /api/keyword/21259 true 21259 true Keyword 21428 /api/keyword/21428 true 21428 true Keyword 1006324 /api/keyword/1006324 true 1006324 true Keyword 1006635 /api/keyword/1006635 true 1006635 true Keyword 1042479 /api/keyword/1042479 true 1042479 true Keyword 1043420 /api/keyword/1043420 true 1043420 true Keyword 1049967 /api/keyword/1049967 true 1049967 true Keyword 1068511 /api/keyword/1068511 true 1068511 true Keyword 1106092 /api/keyword/1106092 true 1106092 true Keyword 1116784 /api/keyword/1116784 true 1116784 true Keyword 1325607 /api/keyword/1325607 true 1325607 true SjrRating 11097825 /api/sjrrating/11097825 3 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 2801 /api/classificationexternal/2801 true 2801 true D1 DIRECT true true /api/publication/2710791 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" >Tonnesen J </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="191"><a href="/gui2/?type=authors&mode=browse&sel=191" target="_blank">Katona G (<span class="authorship-author-name">Katona Gergely</span> <span class="authorAux-mtmt"> Farmakológia</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Pharmacology">DP</span>/Two-photon Imaging Center</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10012926"><a href="/gui2/?type=authors&mode=browse&sel=10012926" target="_blank">Rozsa B (<span class="authorship-author-name">Rózsa J. Balázs</span> <span class="authorAux-mtmt"> Idegtudomány, fizika</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Pharmacology">DP</span>/Two-photon Imaging Center</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Nagerl UV ✉ </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;2710791" target="_blank">Spine neck plasticity regulates compartmentalization of synapses.</a></div> <div> <span class="journal-title">NATURE NEUROSCIENCE</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1097-6256">1097-6256</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1546-1726">1546-1726</a>)</span>: <span class="journal-volume">17</span> <span class="journal-issue">5</span> <span class="page"> pp 678-685 </span> <span class="year">(2014)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_PAY" title=" Pay "> <a style="color:blue" title="10.1038/nn.3682" target="_blank" href="https://doi.org/10.1038/nn.3682"> DOI </a> </span> <span class="id identifier oa_GREEN" title=" Green "> <a style="color:black" title="22358" target="_blank" href="https://real.mtak.hu/22358"> REAL </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000335016200011" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000335016200011"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="261033645" target="_blank" href="https://www.researchgate.net/publication/261033645"> ResearchGate publ. </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="84899633952" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84899633952"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="24657968" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=24657968&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Neuroscience (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;D1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 375</span> | Independent citation: 347 | Self citation: 28 | Unknown citation: 0 | Number of citations in WoS: 342 | Number of citations in Scopus:&nbsp;349 | WoS/Scopus assigned:&nbsp;371 | Number of citations with DOI:&nbsp;363 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;2710791&sort=publishedYear,desc&sort=title"> Number of cited publications: 3 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 2710791</span> | <span class="status-data status-APPROVED"> Published </span> <span class="oldId">Old id: 2710791</span> | Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">MEDLINE</span> </div> <div class="lastModified">Last Modified: 2023.03.21. 14:04 Márta Turek (KOKI admin 4) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Comments</u>: Interdisciplinary Institute for Neuroscience (IINS), University of Bordeaux, Bordeaux, France UMR 5297, Centre National de la Recherche Scientifique (CNRS), Bordeaux, France Two-photon Imaging Center, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary Cited By :266 E...</pre> </div></div>