Development of efficient screening methods has increasing significance in rapid evaluation
of novel biocatalysts. Our study reveals the scopes and limitations of a novel GC-based
multi-substrate screening method for initial characterization of the activity and
selectivity of lipase biocatalysts. The multi-substrate kinetic resolution of four
different racemic alcohols 1-4a by native and immobilized biocatalysts were analyzed
by GC using enantiomer selective stationary phase.