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677-712; \n\nGhosh, S., Singh, A., Mandal, S., Mandal, L., Active hematopoietic hubs in Drosophila adults generate hemocytes and contribute to immune response (2015) Dev Cell, 33 (4), pp. 478-488; \n\nLeitao, A.B., Sucena, E., Drosophila sessile hemocyte clusters are true hematopoietic tissues that regulate larval blood cell differentiation (2015) Elife, 4; \n\nMakhijani, K., Alexander, B., Tanaka, T., Rulifson, E., Bruckner, K., The peripheral nervous system supports blood cell homing and survival in the Drosophila larva (2011) Development, 138 (24), pp. 5379-5391; \n\nCrozatier, M., Ubeda, J.M., Vincent, A., Meister, M., Cellular immune response to parasitization in Drosophila requires the EBF orthologue collier (2004) Plos Biol, 2 (8), p. 196; \n\nMarkus, R., Sessile hemocytes as a hematopoietic compartment in Drosophila melanogaster (2009) Proc Natl Acad Sci U S A, 106 (12), pp. 4805-4809; \n\nMinakhina, S., Steward, R., Melanotic mutants in Drosophila: Pathways and phenotypes (2006) Genetics, 174 (1), pp. 253-263; \n\nBina, S., Wright, V.M., Fisher, K.H., Milo, M., Zeidler, M.P., Transcriptional targets of Drosophila JAK/STAT pathway signalling as effectors of haematopoietic tumour formation (2010) EMBO Rep, 11 (3), pp. 201-207; \n\nAvet-Rochex, A., An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis (2010) BMC Dev Biol, 10, p. 65; \n\nRodriguez, A., Identification of immune system and response genes, and novel mutations causing melanotic tumor formation in Drosophila melanogaster (1996) Genetics, 143 (2), pp. 929-940; \n\nMandal, L., Banerjee, U., Hartenstein, V., Evidence for a fruit fly hemangioblast and similarities between lymph-gland hematopoiesis in fruit fly and mammal aorta-gonadal-mesonephros mesoderm (2004) Nat Genet, 36 (9), pp. 1019-1023; \n\nGrigorian, M., Mandal, L., Hakimi, M., Ortiz, I., Hartenstein, V., The convergence of Notch and MAPK signaling specifies the blood progenitor fate in the Drosophila mesoderm (2011) Dev Biol, 353 (1), pp. 105-118; \n\nLebestky, T., Jung, S.H., Banerjee, U.A., Serrate-expressing signaling center controls Drosophila hematopoiesis (2003) Genes Dev, 17 (3), pp. 348-353; \n\nKrzemien, J., Control of blood cell homeostasis in Drosophila larvae by the posterior signalling centre (2007) Nature, 446 (7133), pp. 325-328; \n\nMandal, L., Martinez-Agosto, J.A., Evans, C.J., Hartenstein, V., Banerjee, U.A., Hedgehog- and Antennapedia-dependent niche maintains Drosophila haematopoietic precursors (2007) Nature, 446 (7133), pp. 320-324; \n\nBenmimoun, B., Polesello, C., Haenlin, M., Waltzer, L., The EBF transcription factor Collier directly promotes Drosophila blood cell progenitor maintenance independently of the niche (2015) Proc Natl Acad Sci U S A, 112 (29), pp. 9052-9057; \n\nSinenko, S.A., Mandal, L., Martinez-Agosto, J.A., Banerjee, U., Dual role of wingless signaling in stem-like hematopoietic precursor maintenance in Drosophila (2009) Dev Cell, 16 (5), pp. 756-763; \n\nPennetier, D., Size control of the Drosophila hematopoietic niche by bone morphogenetic protein signaling reveals parallels with mammals (2012) Proc Natl Acad Sci U S A, 109 (9), pp. 3389-3394; \n\nDragojlovic-Munther, M., Martinez-Agosto, J.A., Extracellular matrix-modulated Heartless signaling in Drosophila blood progenitors regulates their differentiation via a Ras/ETS/FOG pathway and target of rapamycin function (2013) Dev Biol, 384 (2), pp. 313-330; \n\nFerguson, G.B., Martinez-Agosto, J.A., Yorkie and Scalloped signaling regulates Notch-dependent lineage specification during Drosophila hematopoiesis (2014) Curr Biol, 24 (22), pp. 2665-2672; \n\nMilton, C.C., The Hippo pathway regulates hematopoiesis in Drosophila melanogaster (2014) Curr Biol, 24 (22), pp. 2673-2680; \n\nEvans, C.J., Liu, T., Banerjee, U., Drosophila hematopoiesis: Markers and methods for molecular genetic analysis (2014) Methods, 68 (1), pp. 242-251; \n\nNeyen, C., Bretscher, A.J., Binggeli, O., Lemaitre, B., Methods to study Drosophila immunity (2014) Methods, 68 (1), pp. 116-128; \n\nSmall, C., Paddibhatla, I., Rajwani, R., Govind, S., An introduction to parasitic wasps of Drosophila and the antiparasite immune response (2012) J Vis Exp., 63, p. 3347; \n\nPetraki, S., Alexander, B., Bruckner, K., Assaying Blood Cell Populations of the Drosophila melanogaster Larva (2015) J Vis Exp., 105; \n\nRizki, M.T.M., Rizki, R.M., Functional significance of the crystal cells in the larva of Drosophila mekmogaster (1959) Journal of Biophysical and Biochemical Cytology, 5, pp. 235-240; \n\nKurucz, E., Definition of Drosophila hemocyte subsets by cell-type specific antigens (2007) Acta Biol Hung, 58, pp. 95-111; \n\nJung, S.H., Evans, C.J., Uemura, C., Banerjee, U., The Drosophila lymph gland as a developmental model of hematopoiesis (2005) Development, 132 (11), pp. 2521-2533; \n\nKrzemien, J., Crozatier, M., Vincent, A., Ontogeny of the Drosophila larval hematopoietic organ, hemocyte homeostasis and the dedicated cellular immune response to parasitism (2010) Int J Dev Biol, 54 (6-7), pp. 1117-1125; \n\nRizki, T.M., Rizki, R.M., Properties of the Larval Hemocytes of Drosophila-Melanogaster (1980) Experientia, 36 (10), pp. 1223-1226; \n\nLanot, R., Zachary, D., Holder, F., Meister, M., Postembryonic hematopoiesis in Drosophila (2001) Dev Biol, 230 (2), pp. 243-257; \n\nMcBrayer, Z., Prothoracicotropic hormone regulates developmental timing and body size in Drosophila (2007) Dev Cell, 13 (6), pp. 857-871; \n\nReimels, T.A., Pfleger, C.M., Drosophila Rabex-5 restricts Notch activity in hematopoietic cells and maintains hematopoietic homeostasis (2015) J Cell Sci, 128 (24), pp. 4512-4525", "hasCitationDuplums" : false, "userChangeableUntil" : "2017-08-23T10:13:11.000+0000", "publishDate" : "2017-07-19T11:57:08.000+0000", "directInstitutesForSort" : "", "ownerAuthorCount" : 12, "ownerInstituteCount" : 13, 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