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A 99.8% of the starch was removed by a\ntwo-step enzymtic hydrolysis. The remaining solid fraction was\npretreated with alkali (2.5% NaOH) and alkaline peroxide (2.5% NaOH,\n0.6% H2O2), in order to isolate corn fibre gum (CFG). The highest yield\ncould be obtained under the most severe conditions (alkaline peroxide,\n120degreesC, 120 min). Following the pretreatment 51.3% of the corn\nfibre gum could be isolated by alcoholic precipitation from the soluble\nfraction. The solid fraction was used as carbon source for cellulase\nenzyme fermentation and as substrate for enzymatic hydrolysis. A 30%\nhigher filter paper activity was obtained using pre-treated corn fibre\nas carbon source compared to the activities reached on original and\ndestarched corn fibre. However, 15% higher filter paper activities were\nobtained on Solka Floe than on the pre-treated corn fibre samples. The\nenzymes produced were used for the hydrolysis of the pre-treated corn\nfibre substrate. 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