In RNA Mapping- Methods and Protocols expert researchers in the field detail many
of the methods which are now commonly used to study RNA. These include protocols for
the consequence of the emerging interest in the characterization of cellular RNAs
urged by their potential use as diagnostic biomarkers or therapeutic targets. In particular,
the biological relevance of microRNAs in human physiology and disease development
is highlighted in the 16 chapters focused on methods for their physical and functional
mapping. Written in the highly successful Methods in Molecular Biology series format,
chapters include introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible laboratory protocols, and
key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, RNA Mapping- Methods and Protocols provides instruction
and inspiration for scientists who are facing the challenges of the discovery and/or
functional characterization of RNA molecules for a wide variety of applications ranging
from novel biomedical diagnostics to therapeutics and biomaterials.
