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Our experiments were aimed at exploring whether synthetic γ-lactones had such effects. None of the seven γ-lactones studied showed antibacterial activity, but two of them inhibited β-lactamases isolated from various bacteria. These two γ-lactones did not reduce colony formation of murine bone marrow cells in vitro, indicating that they were not toxic to proliferating mammalian cells. Four γ-lactones, including the two inhibiting β-lactamase, stimulated sporulation in the non-sporulating S. griseus bald 7 mutant. 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