Recruitment of Perisomatic Inhibition during Spontaneous Hippocampal Activity

Beyeler, A; Retailleau, A; Molter, C; Mehidi, A; Szabadics, J [Szabadics, János (Celluláris neurof...), author] MTA Kísérleti Orvostudományi Kutatóintézet; Laboratory of Cellular Neuropharmacology (IEM / DP); Laboratory of Cellular Neuropharmacology; Leinekugel, X

English Article (Journal Article) Scientific
Published: PLOS ONE 1932-6203 1932-6203 8 (6) Paper: e66509 2013
  • Pedagógiai Tudományos Bizottság: A
  • Szociológiai Tudományos Bizottság: A nemzetközi
  • Regionális Tudományok Bizottsága: B nemzetközi
  • SJR Scopus - Agricultural and Biological Sciences (miscellaneous): D1
Subjects:
  • Biological sciences
  • Other agricultural sciences
  • Other medical sciences
  • Other natural sciences
  • Clinical medicine
It was recently shown that perisomatic GABAergic inhibitory postsynaptic potentials (IPSPs) originating from basket and chandelier cells can be recorded as population IPSPs from the hippocampal pyramidal layer using extracellular electrodes (eIPSPs). Taking advantage of this approach, we have investigated the recruitment of perisomatic inhibition during spontaneous hippocampal activity in vitro. Combining intracellular and extracellular recordings from pyramidal cells and interneurons, we confirm that inhibitory signals generated by basket cells can be recorded extracellularly, but our results suggest that, during spontaneous activity, eIPSPs are mostly confined to the CA3 rather than CA1 region. CA3 eIPSPs produced the powerful time-locked inhibition of multi-unit activity expected from perisomatic inhibition. Analysis of the temporal dynamics of spike discharges relative to eIPSPs suggests significant but moderate recruitment of excitatory and inhibitory neurons within the CA3 network on a 10 ms time scale, within which neurons recruit each other through recurrent collaterals and trigger powerful feedback inhibition. Such quantified parameters of neuronal interactions in the hippocampal network may serve as a basis for future characterisation of pathological conditions potentially affecting the interactions between excitation and inhibition in this circuit.
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2024-07-18 00:39