Technological advances in the field of microarray production and analysis lead to
the development of protein microarrays. Of these, antigen microarrays are one particular
format that allows the study of antigen-antibody interactions in a miniaturized and
highly multiplexed fashion. Here, we describe the parallel detection of antibodies
with different specificities in human serum, a procedure also called antibody profiling.
Autoantigens printed on microarray slides are reacted with test sera and the bound
antibodies are identified by fluorescently labeled secondary reagents. Reactivity
patterns generated this way characterize individuals and can help design novel diagnostic
tools.
