https://m2.mtmt.hu/ eng 2026-10-25 11:57 JournalArticle 2015276 APPROVED true Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary Two-Photon Imaging Center, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary Institute of Anatomy and Cell Biology, Department of Neuroanatomy, University of Freiburg, Freiburg, Germany Centre for Biological Signaling Studies, University of Freiburg, Freiburg, Germany Department of Anatomy, Hokkaido University School of Medicine, Sapporo, Japan Cited By :288 Export Date: 12 June 2023 CODEN: NANEF Correspondence Address: Nusser, Z.; Laboratory of Cellular Neurophysiology, , Budapest, Hungary; email: nusser@koki.hu Chemicals/CAS: glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; Calcium Channels, N-Type; voltage-dependent calcium channel (P-Q type) Funding details: 1.1.1-08/1-2008-0085 Funding details: Wellcome Trust, WT, 083484/Z/07/Z, 090197/Z/09/Z, 094513/Z/10/Z Funding details: Seventh Framework Programme, FP7, 293681 Funding details: European Research Council, ERC Funding details: Deutsche Forschungsgemeinschaft, DFG, SFB 780 Funding details: Magyar Tudományos Akadémia, MTA Funding text 1: N.H. and A.L. are funded by Janos Bolyai Scholarships of the Hungarian Academy of Sciences. Z.N. is supported by Wellcome Trust Equipment (083484/Z/07/Z) and Project Grants (090197/Z/09/Z; 094513/Z/10/Z), a European Research Council Advanced Grant, and a Hungarian National Office for Research and Technology-French National Research Agency TéT Fund (NKTH-Neurogen). B.R. was supported by a GOP grant (1.1.1-08/1-2008-0085). A.K. is supported by a Deutsche Forschungsgemeinschaft (SFB 780) grant. We thank N. Suzuki (Mie University, Japan) for providing Cav2.1−/− mice, A. Unger for helping with the Cav2.1−/− replica labeling, E. Dobai and D. Ronaszéki for technical assistance, M. Sümegi for his help with the modeling, members of Synaptic Systems GmbH for providing the rabbit anti-Cav2.1 antibody, E. Neher and M. Eyre for their comments on the manuscript, and H. Koester for helpful discussions. 0 2018-09-30T14:01:47.000+0000 false 2015276 2026-10-16T10:13:15.668+0000 2022-11-30T09:27:12.790+0000 2012-07-09T23:58:32.000+0000 true 10003154 Wolf György /api/admin/10003154 Admin true 10003154 2025-04-16T09:23:57.809+0000 2025-04-16T09:23:57.809+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Holderith, N Release probability of hippocampal glutamatergic terminals scales with the size of the active zone true true /api/journal/19517 REVIEWED true false 19517 false 19517 true 1097-6256 1546-1726 Journal FOREIGN 15 7 988 997 988 10 2012 Cortical synapses have structural, molecular and functional heterogeneity; our knowledge regarding the relationship between their ultrastructural and functional parameters is still fragmented. Here we asked how the neurotransmitter release probability and presynaptic [Ca(2+)] transients relate to the ultrastructure of rat hippocampal glutamatergic axon terminals. Two-photon Ca(2+) imaging-derived optical quantal analysis and correlated electron microscopic reconstructions revealed a tight correlation between the release probability and the active-zone area. Peak amplitude of [Ca(2+)] transients in single boutons also positively correlated with the active-zone area. Freeze- fracture immunogold labeling revealed that the voltage-gated calcium channel subunit Cav2.1 and the presynaptic protein Rim1/2 are confined to the active zone and their numbers scale linearly with the active-zone area. Gold particles labeling Cav2.1 were nonrandomly distributed in the active zones. Our results demonstrate that the numbers of several active-zone proteins, including presynaptic calcium channels, as well as the number of docked vesicles and the release probability, scale linearly with the active-zone area. true true 2012 789360 true true true false true false GREEN GREEN 2026-10-16 true https://real.mtak.hu/86115 true 10012931 Lőrincz Andrea /api/author/10012931 Author true 10012931 365 0 364 337 35 360 364 348 357 363 363 336 336 338 27 0 365 338 364 337 0 7 7 Folyóiratcikk Journal Article 24 358 Könyvrészlet Chapter in Book 25 6 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 1 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2007 0 2 2 2 2 2008 0 0 0 0 0 2009 0 0 0 0 0 2010 0 0 0 0 0 2011 0 6 6 6 6 2012 0 7 6 7 6 2013 0 29 28 29 28 2014 0 24 21 24 21 2015 0 37 34 37 34 2016 0 38 35 38 35 2017 0 27 23 27 23 2018 0 34 30 34 30 2019 0 27 25 27 25 2020 0 29 28 29 28 2021 0 37 36 37 36 2022 0 25 24 25 24 2023 0 18 18 18 18 2024 0 15 14 15 14 2025 0 9 7 9 7 2026 0 1 1 1 1 D1 false 10000499 false false false 2012-11-09T13:26:37.000+0000 2012-07-10T00:09:39.000+0000 Celluláris Idegélettani Kutatócsoport (HRN KOKI); Két-foton Képalkotó Központ (KOKI / GYO) 5 26 2 7 0 true Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 2953687 /api/authorship/2953687 1 0.14285715 true false Author 10011496 /api/author/10011496 Holderith Noémi true 10011496 true Holderith N true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 15395294 true PersonAuthorship 2953688 /api/authorship/2953688 2 0.14285715 false false Author 10012931 /api/author/10012931 Lőrincz Andrea true 10012931 true Lorincz A true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 15395293 true PersonAuthorship 2953689 /api/authorship/2953689 3 0.14285715 false false Author 191 /api/author/191 Katona Gergely true 191 true Katona G true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 15395292 true PersonAuthorship 2953690 /api/authorship/2953690 4 0.14285715 false false Author 10012926 /api/author/10012926 Rózsa J. Balázs true 10012926 true Rozsa B true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 15395291 true PersonAuthorship 2953691 /api/authorship/2953691 5 0.14285715 false false Kulik A true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 15395290 true PersonAuthorship 2953692 /api/authorship/2953692 6 0.14285715 false false Watanabe M true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 15395289 true PersonAuthorship 2953693 /api/authorship/2953693 7 0.143 false true true Author 10000499 /api/author/10000499 Nusser Zoltán true 10000499 true Nusser Z true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 15395288 true PublicationIdentifier 548529 /api/publicationidentifier/548529 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true false IDENTICAL 10.1038/nn.3137 https://doi.org/10.1038/nn.3137 false 336362 true PublicationIdentifier 548531 /api/publicationidentifier/548531 SwordSource 36 /api/publicationsource/36 PublicationSourceType 10007 /api/publicationsourcetype/10007 true true true REAL REAL https://real.mtak.hu/@@@ true true 36 true GREEN true NO 86115 https://real.mtak.hu/86115 false 1845589 true PublicationIdentifier 548527 /api/publicationidentifier/548527 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true false IDENTICAL 000305819600017 https://www.webofscience.com/wos/woscc/full-record/000305819600017 false 614529 true PublicationIdentifier 548528 /api/publicationidentifier/548528 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true false IDENTICAL 84862860810 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84862860810 false 1547549 true PublicationIdentifier 548530 /api/publicationidentifier/548530 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true false NO 22683683 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=22683683&dopt=Abstract false 336363 true Classification 12125 /api/classification/12125 true true Keyword 12 /api/keyword/12 true 12 true Keyword 62 /api/keyword/62 true 62 true Keyword 1547 /api/keyword/1547 true 1547 true Keyword 3436 /api/keyword/3436 true 3436 true Keyword 6763 /api/keyword/6763 true 6763 true Keyword 6915 /api/keyword/6915 true 6915 true Keyword 15440 /api/keyword/15440 true 15440 true Keyword 1330830 /api/keyword/1330830 true 1330830 true Keyword 1330831 /api/keyword/1330831 true 1330831 true Keyword 1330832 /api/keyword/1330832 true 1330832 true SjrRating 5671822 /api/sjrrating/5671822 3 0.1 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 2801 /api/classificationexternal/2801 true 2801 true D1 DIRECT true 5671822 true /api/publication/2015276 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10011496"><a href="/gui2/?type=authors&mode=browse&sel=10011496" target="_blank">Holderith N (<span class="authorship-author-name">Holderith Noémi</span> <span class="authorAux-mtmt"> Neurobiológia</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Cellular and Network Neurobiology">DCNN</span>/Laboratory of Cellular Neurophysiology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10012931"><a href="/gui2/?type=authors&mode=browse&sel=10012931" target="_blank">Lorincz A (<span class="authorship-author-name">Lőrincz Andrea</span> <span class="authorAux-mtmt"> Neuroanatómia</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Cellular and Network Neurobiology">DCNN</span>/Laboratory of Cellular Neurophysiology</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="191"><a href="/gui2/?type=authors&mode=browse&sel=191" target="_blank">Katona G (<span class="authorship-author-name">Katona Gergely</span> <span class="authorAux-mtmt"> Farmakológia</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Pharmacology">DP</span>/Two-photon Imaging Center</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10012926"><a href="/gui2/?type=authors&mode=browse&sel=10012926" target="_blank">Rozsa B (<span class="authorship-author-name">Rózsa J. Balázs</span> <span class="authorAux-mtmt"> Idegtudomány, fizika</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Pharmacology">DP</span>/Two-photon Imaging Center</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Kulik A </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Watanabe M </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000499"><a href="/gui2/?type=authors&mode=browse&sel=10000499" target="_blank">Nusser Z ✉ (<span class="authorship-author-name">Nusser Zoltán</span> <span class="authorAux-mtmt"> Celluláris idegélettan</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Cellular and Network Neurobiology">DCNN</span>/Laboratory of Cellular Neurophysiology</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;2015276" target="_blank">Release probability of hippocampal glutamatergic terminals scales with the size of the active zone</a></div> <div> <span class="journal-title">NATURE NEUROSCIENCE</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1097-6256">1097-6256</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1546-1726">1546-1726</a>)</span>: <span class="journal-volume">15</span> <span class="journal-issue">7</span> <span class="page"> pp 988-997 </span> <span class="year">(2012)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.1038/nn.3137" target="_blank" href="https://doi.org/10.1038/nn.3137"> DOI </a> </span> <span class="id identifier oa_GREEN" title=" Green "> <a style="color:black" title="86115" target="_blank" href="https://real.mtak.hu/86115"> REAL </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000305819600017" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000305819600017"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="84862860810" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-84862860810"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="22683683" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=22683683&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Neuroscience (miscellaneous)&nbsp;&nbsp;&nbsp;Rank:&nbsp;D1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 365</span> | Independent citation: 338 | Self citation: 27 | Unknown citation: 0 | Number of citations in WoS: 348 | Number of citations in Scopus:&nbsp;357 | WoS/Scopus assigned:&nbsp;363 | Number of citations with DOI:&nbsp;364 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;2015276&sort=publishedYear,desc&sort=title"> Number of cited publications: 7 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 2015276</span> | <span class="status-data status-APPROVED"> Published </span> <span class="oldId">Old id: 2015276</span> | Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">MEDLINE</span> </div> <div class="lastModified">Last Modified: 2022.11.30. 10:27 Márta Turek (KOKI admin 4) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Comments</u>: Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary Two-Photon Imaging Center, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary Institute of Anatomy and Cell Biology, Department of Neuroanatomy, University of Freiburg, Freiburg, Germany ...</pre> </div></div>