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These results were utilized to develop an analytical method for the simultaneous determination of H2O2 and organic hydroperoxides in aqueous solutions. The total amount of H2O2 and organic hydroperoxides can be determined by the spectrophotometric measurement of I-3(-) formed quantitatively during 30 min of heating at 60degreesC. Catalase selectively decomposes H2O2 in solutions containing organic hydroperoxides. The total amount of the latter can therefore be determined iodometrically after H2O2 decomposition. In the oxidation of leuco crystal violet to crystal violet by H2O2 and organic hydroperoxides, horseradish peroxidase exerts similar activities in the reactions involving methyl and ethyl hydroperoxides and H2O2 , but its activity is much lower with tert -butyl and cumene hydroperoxide. 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