https://m2.mtmt.hu/ hun 2026-10-23 04:49 JournalArticle 1629372 VALIDATED true Funding Agency and Grant Number: Hungarian Scientific Research FundOrszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [PD73064]; European Social FundEuropean Social Fund (ESF); European Regional Development FundEuropean Commission; [TAMOP 4.2.1./B-09/1/KONV-2010-0007]; NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Dental & Craniofacial Research (NIDCR) [R01DE016291] Funding Source: NIH RePORTER Funding text: This work was supported by the Hungarian Scientific Research Fund (Project No. PD73064) and by the TAMOP 4.2.1./B-09/1/KONV-2010-0007 project. The project is implemented through the New Hungary Development Plan, co-financed by the European Social Fund and the European Regional Development Fund. 0 2014-10-16T11:08:48.000+0000 false 1629372 2026-10-14T19:36:21.063+0000 2025-07-01T20:12:54.444+0000 2011-07-12T16:35:11.000+0000 true 1010831 Antus Sándor /api/author/1010831 Author true 1010831 2025-07-01T20:12:54.631+0000 2025-07-01T20:12:54.631+0000 2023-07-08T17:42:23.117+0000 true 10036259 Zékány László /api/admin/10036259 Admin true 10036259 Admin 10063514 /api/admin/10063514 Mikola Katalin true 10063514 true 2023-08-11T08:11:43.622+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Fekete, A Synthesis of beta-(1->6)-linked N-acetyl-D-glucosamine oligosaccharide substrates and their hydrolysis by Dispersin B true true /api/journal/917 REVIEWED true false 917 false 917 true 0008-6215 1873-426X Journal FOREIGN 346 12 1445 1453 1445 9 2011 Dispersin B (DspB) from Aggregatibacter actinomycetemcomitans is a p-hexosaminidase exhibiting biofilm detachment activity. A series of beta-(1 -> 6)-linked N-acetyl-D-glucosamine thiophenyl glycosides with degree of polymerisation (DP) of 2, 3, 4 and 5 were synthesized, and substrate specificity of DspB was studied on the obtained oligosaccharides. For oligomer synthesis a 1+2, 2+2, 1+4 coupling strategy was applied, using bromo-sugars as glycosyl donors. The formation of 1,2-trans interglycosidic bond has been ensured by 2-phtalimido protecting group: chloroacetyl group was installed to mask temporarily the 6-hydroxyl and acetate esters were applied as permanent protecting groups. Enzymatic studies revealed that DP of the GlcNAc oligomers strongly affected the hydrolysis rate, and the hydrolytic activity of DspB on the tetramer and pentamer have been found to be approximately 10-fold higher than that of the dimer. This fact indicates that four units are required for a strong binding at the active centre of DspB. The role of aromatic amino acids W237, Y187 and Y278 in substrate specificity and catalysis was also examined using mutant enzymes. (C) 2011 Elsevier Ltd. 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Sándor true 1010831 true Antus S true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 13064242 true Institute 19040 /api/institute/19040 0 false false true Akadémikus bibliográfia Bibliografy of Hungarian of Academy of Sciences' academicians true 19040 true PublicationIdentifier 394596 /api/publicationidentifier/394596 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GREEN true IDENTICAL 10.1016/j.carres.2011.03.029 https://doi.org/10.1016/j.carres.2011.03.029 false 231439 true PublicationIdentifier 394594 /api/publicationidentifier/394594 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true false IDENTICAL 000292852100010 https://www.webofscience.com/wos/woscc/full-record/000292852100010 false 231440 true 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/api/publication/1629372 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="10012839"><a href="/gui2/?type=authors&mode=browse&sel=10012839" target="_blank">Fekete A (<span class="authorship-author-name">Fekete Anikó</span> <span class="authorAux-mtmt"> Szerves kémia, szénhidrátkémia</span>) </a> </span> <span class="author-affil"><span title="MTA Támogatott Kutatócsoportok">MTA TKI</span>/MTA-DE Szénhidrát-kémiai Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10002015"><a href="/gui2/?type=authors&mode=browse&sel=10002015" target="_blank">Borbás A (<span class="authorship-author-name">Borbás Anikó</span> <span class="authorAux-mtmt"> Szerves kémia, Gyógyszerészi kémia</span>) </a> </span> <span class="author-affil"><span title="MTA Támogatott Kutatócsoportok">MTA TKI</span>/MTA-DE Szénhidrát-kémiai Kutatócsoport</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10002900"><a href="/gui2/?type=authors&mode=browse&sel=10002900" target="_blank">Gyémánt Gy ✉ (<span class="authorship-author-name">Gyémánt Gyöngyi</span> <span class="authorAux-mtmt"> Kromatográfia, biokémia, szénhidrátkémia</span>) </a> </span> <span class="author-affil"><span title="Debreceni Egyetem">DE</span>/<span title="Természettudományi és Technológiai Kar">TTK</span>/<span title="Kémiai Intézet">KemI</span>/Szervetlen és Analitikai Kémiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10007645"><a href="/gui2/?type=authors&mode=browse&sel=10007645" target="_blank">Kandra L (<span class="authorship-author-name">Kandra Lili</span> <span class="authorAux-mtmt"> Biokémia</span>) </a> </span> <span class="author-affil"><span title="Debreceni Egyetem">DE</span>/<span title="Természettudományi és Technológiai Kar">TTK</span>/<span title="Kémiai Intézet">KemI</span>/Szervetlen és Analitikai Kémiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10037465"><a href="/gui2/?type=authors&mode=browse&sel=10037465" target="_blank">Fazekas E (<span class="authorship-author-name">Fazekas Erika</span> <span class="authorAux-mtmt"> Biokémia</span>) </a> </span> <span class="author-affil"><span title="Debreceni Egyetem">DE</span>/<span title="Természettudományi és Technológiai Kar">TTK</span>/<span title="Kémiai Intézet">KemI</span>/Szervetlen és Analitikai Kémiai Tanszék</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Ramasubbu N </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="1010831"><a href="/gui2/?type=authors&mode=browse&sel=1010831" target="_blank">Antus S (<span class="authorship-author-name">Antus Sándor</span> <span class="authorAux-mtmt"> Szerves kémia</span>) </a> </span> <span class="author-affil"><span title="MTA Támogatott Kutatócsoportok">MTA TKI</span>/MTA-DE Szénhidrát-kémiai Kutatócsoport; <span title="Debreceni Egyetem">DE</span>/<span title="Tudományegyetemi Karok">TEK</span>/<span title="Természettudományi és Technológiai Kar">TTK</span>/<span title="Kémiai Intézet">KemI</span>/Szerves Kémiai Tanszék</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;1629372" target="_blank">Synthesis of beta-(1->6)-linked N-acetyl-D-glucosamine oligosaccharide substrates and their hydrolysis by Dispersin B</a></div> <div> <span class="journal-title">CARBOHYDRATE RESEARCH</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0008-6215">0008-6215</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1873-426X">1873-426X</a>)</span>: <span class="journal-volume">346</span> <span class="journal-issue">12</span> <span class="page"> pp 1445-1453 </span> <span class="year">(2011)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GREEN" title=" Green "> <a style="color:blue" title="10.1016/j.carres.2011.03.029" target="_blank" href="https://doi.org/10.1016/j.carres.2011.03.029"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000292852100010" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000292852100010"> WoS </a> </span> <span class="id identifier oa_CLOSED" title=" Zárt "> <a style="color:blue" title="109976" target="_blank" href="http://hdl.handle.net/2437/109976"> DEA </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="79959962700" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-79959962700"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> 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class="lastModified">Utolsó módosítás: 2025.07.01. 22:12 Ladányi Gusztáv (MTMT API user, admin) </div> <div class="lockedBy">Központi kezelésű 2023.08.11. 10:11 Mikola Katalin (MTMT 3, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Megjegyzés</u>: Funding Agency and Grant Number: Hungarian Scientific Research FundOrszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [PD73064]; European Social FundEuropean Social Fund (ESF); European Regional Development FundEuropean Commission; [TAMOP 4.2.1./B-09/1/KONV-2010-0007]; NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCHUnited States Department of Health & Human ServicesNational Institutes of He...</pre> </div></div>