Ligand-induced changes in oestrogen and thyroid hormone receptor expression in the developing rat cerebellum: a comparative quantitative PCR and Western blot study.

J Scalise, Trudy; Gyorffy, Andrea [Győrffy, Andrea (Energia-metabolizmus), szerző] Élettani és biokémiai tanszék (SZIE / ÁOTK); Toth, Istvan [Tóth, István (Állatorvos-élettan), szerző] Élettani és biokémiai tanszék (SZIE / ÁOTK); S Kiss, David [Kiss, Dávid Sándor (neurobiológia), szerző] Élettani és biokémiai tanszék (SZIE / ÁOTK); Somogyi, Virag [Somogyi, Virág (Immunológia), szerző] Élettani és biokémiai tanszék (SZIE / ÁOTK); Goszleth, Greta [Goszleth, Gréta (Állatorvosi élettan), szerző] Élettani és biokémiai tanszék (SZIE / ÁOTK); Bartha, Tibor [Bartha, Tibor (Állatorvosi élettan), szerző] Élettani és biokémiai tanszék (SZIE / ÁOTK); Frenyo, V Laszlo [Frenyó V., László (Állatorvosi élettan), szerző] Élettani és biokémiai tanszék (SZIE / ÁOTK); Zsarnovszky, Attila [Zsarnovszky, Attila (Állatorvos tudomány), szerző] Élettani és biokémiai tanszék (SZIE / ÁOTK)

Angol nyelvű Tudományos Szakcikk (Folyóiratcikk)
Megjelent: ACTA VETERINARIA HUNGARICA 0236-6290 60 (2) pp. 263-284 Paper: DBIO-11-250 2012
  • SJR Scopus - Veterinary (miscellaneous): Q2
Azonosítók
Szakterületek:
    Oestrogen (E2) and thyroid hormones (THs) are key regulators of cerebel- lar development. Recent reports implicate a complex mechanism through which E2 and THs influence the expression levels of each other’s receptors (ERs and TRs) to precisely mediate developmental signals and modulate signal strength. We examined the modulating effects of E2 and THs on the expression levels of their receptor mRNAs and proteins in cultured cerebellar cells obtained from 7- day-old rat pups. Cerebellar granule cell cultures were treated with either E2, THs or a combination of these hormones, and resulting receptor expression levels were determined by quantitative PCR and Western blot techniques. The results were compared to non-treated controls and to samples obtained from 14-day-old in situ cerebella. Additionally, we determined the glial effects on the regulation of ER- TR expression levels. The results show that (i) ER and TR expression depends on the combined presence of E2 and THs; (ii) glial cells mediate the hormonal regu- lation of neuronal ER-TR expression, and (iii) loss of tissue integrity results in characteristic changes in ER-TR expression levels. These observations suggest that both E2 and THs, in adequate amounts, are required for the precise orchestra- tion of cerebellar development and that alterations in the ratio of E2/THs may in- fluence signalling mechanisms involved in neurodevelopment. Comparison of data from in vitro and in situ samples revealed a shift in receptor expression levels after loss of tissue integrity, suggesting that such adjusting/regenerative mecha- nisms may function after cerebellar tissue injury as well.
    Hivatkozás stílusok: IEEEACMAPAChicagoHarvardCSLMásolásNyomtatás
    2021-03-03 22:10