https://m2.mtmt.hu/ hun 2026-05-01 06:05 JournalArticle 1498567 ADMIN_APPROVED true 0 2015-03-16T10:17:45.000+0000 false 1498567 2026-02-03T13:11:05.474+0000 2022-09-07T10:22:19.921+0000 2011-04-11T17:18:01.000+0000 true 10021050 Csúcs Gábor /api/author/10021050 Author true 10021050 2022-09-07T10:22:07.729+0000 2022-09-07T10:22:07.729+0000 2022-09-07T10:22:20.157+0000 true 10014568 Vándor Andrea /api/admin/10014568 Admin true 10014568 Admin 10014568 /api/admin/10014568 Vándor Andrea true 10014568 true 2022-09-08T06:43:42.919+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Wild, T A Protein Inventory of Human Ribosome Biogenesis Reveals an Essential Function of Exportin 5 in 60S Subunit Export true true /api/journal/10001349 REVIEWED true false 10001349 false 10001349 true 1544-9173 1545-7885 Journal FOREIGN 8 10 e1000522 e1000522 4 2010 The assembly of ribosomal subunits in eukaryotes is a complex, multistep process so far mostly studied in yeast. In S. cerevisiae, more than 200 factors including ribosomal proteins and trans-acting factors are required for the ordered assembly of 40S and 60S ribosomal subunits. To date, only few human homologs of these yeast ribosome synthesis factors have been characterized. Here, we used a systematic RNA interference (RNAi) approach to analyze the contribution of 464 candidate factors to ribosomal subunit biogenesis in human cells. The screen was based on visual readouts, using inducible, fluorescent ribosomal proteins as reporters. By performing computer-based image analysis utilizing supervised machine-learning techniques, we obtained evidence for a functional link of 153 human proteins to ribosome synthesis. Our data show that core features of ribosome assembly are conserved from yeast to human, but differences exist for instance with respect to 60S subunit export. Unexpectedly, our RNAi screen uncovered a requirement for the export receptor Exportin 5 (Exp5) in nuclear export of 60S subunits in human cells. We show that Exp5, like the known 60S exportin Crm1, binds to pre-60S particles in a RanGTP-dependent manner. Interference with either Exp5 or Crm1 function blocks 60S export in both human cells and frog oocytes, whereas 40S export is compromised only upon inhibition of Crm1. Thus, 60S subunit export is dependent on at least two RanGTP-binding exportins in vertebrate cells. 2019 true true true false false false GOLD 2026-02-03 true https://doi.org/10.1371/journal.pbio.1000522 true 10030884 Nemec Évi /api/admin/10030884 Admin true 10030884 171 0 171 147 3 171 170 167 110 171 171 147 147 147 24 0 171 147 171 147 0 1 1 Folyóiratcikk Journal Article 24 167 Könyvrészlet Chapter in Book 25 4 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2011 0 10 9 10 9 2012 0 7 4 7 4 2013 0 18 13 18 13 2014 0 14 11 14 11 2015 0 18 16 18 16 2016 0 14 12 14 12 2017 0 6 5 6 5 2018 0 12 12 12 12 2019 0 13 12 13 12 2020 0 7 6 7 6 2021 0 13 11 13 11 2022 0 13 12 13 12 2023 0 12 11 12 11 2024 0 4 4 4 4 2025 0 10 9 10 9 D1 false false false false 2013-05-24T18:18:00.000+0000 2013-02-23T19:18:00.000+0000 2 9 0 10 0 true 10014568 Language 10002 /api/language/10002 Angol English true 2 true PersonAuthorship 1502473 /api/authorship/1502473 1 0.1 true false Wild T true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 12116112 true PersonAuthorship 1502474 /api/authorship/1502474 2 0.1 false false Author 10030964 /api/author/10030964 Horváth Péter true 10030964 true Horvath P true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 12116111 true PersonAuthorship 1502475 /api/authorship/1502475 3 0.1 false false Wyler E true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 12116110 true PersonAuthorship 1502476 /api/authorship/1502476 4 0.1 false false Widmann B true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 12116109 true PersonAuthorship 1502477 /api/authorship/1502477 5 0.1 false false Badertscher L true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 12116108 true PersonAuthorship 1502478 /api/authorship/1502478 6 0.1 false false Zemp I true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 12116107 true PersonAuthorship 1502479 /api/authorship/1502479 7 0.1 false false Kozak K true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 12116106 true PersonAuthorship 1502480 /api/authorship/1502480 8 0.1 false false Author 10021050 /api/author/10021050 Csúcs Gábor true 10021050 true Csucs G true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 12116105 true PersonAuthorship 1502481 /api/authorship/1502481 9 0.1 false false Lund E true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 12116104 true PersonAuthorship 1502482 /api/authorship/1502482 10 0.1 false true Kutay U true false false AuthorshipType 1 /api/authorshiptype/1 0 true 0 true false 12116103 true PublicationIdentifier 363879 /api/publicationidentifier/363879 PlainSource 6 /api/publicationsource/6 PublicationSourceType 10001 /api/publicationsourcetype/10001 true true true DOI DOI https://doi.org/@@@ true true 6 true GOLD true NO 10.1371/journal.pbio.1000522 https://doi.org/10.1371/journal.pbio.1000522 false 208534 true PublicationIdentifier 363878 /api/publicationidentifier/363878 PlainSource 1 /api/publicationsource/1 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true false IDENTICAL 000283495100019 https://www.webofscience.com/wos/woscc/full-record/000283495100019 false 208535 true PublicationIdentifier 16299441 /api/publicationidentifier/16299441 PlainSource 3 /api/publicationsource/3 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true NO 79952277141 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-79952277141 false true PublicationIdentifier 16299442 /api/publicationidentifier/16299442 PlainSource 17 /api/publicationsource/17 PublicationSourceType 10003 /api/publicationsourcetype/10003 false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true NO 21048991 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=21048991&dopt=Abstract false true Classification 12125 /api/classification/12125 true true Classification 10545 /api/classification/10545 true true Classification 12713 /api/classification/12713 true true SjrRating 5732481 /api/sjrrating/5732481 1 0.0 journal RatingType 10002 /api/ratingtype/10002 sjr true true ClassificationExternal 1101 /api/classificationexternal/1101 true 1101 true D1 DIRECT true 5732481 true /api/publication/1498567 <div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" >Wild T </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10030964"><a href="/gui2/?type=authors&mode=browse&sel=10030964" target="_blank">Horvath P (<span class="authorship-author-name">Horváth Péter</span> <span class="authorAux-mtmt"> biokémia,informatika</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Wyler E </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Widmann B </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Badertscher L </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Zemp I </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Kozak K </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10021050"><a href="/gui2/?type=authors&mode=browse&sel=10021050" target="_blank">Csucs G (<span class="authorship-author-name">Csúcs Gábor</span> <span class="authorAux-mtmt"> Biofizika, sejtbiológia, nanotechnológia</span>) </a> </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Lund E </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Kutay U </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;1498567" target="_blank">A Protein Inventory of Human Ribosome Biogenesis Reveals an Essential Function of Exportin 5 in 60S Subunit Export</a></div> <div> <span class="journal-title">PLOS BIOLOGY</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1544-9173">1544-9173</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1545-7885">1545-7885</a>)</span>: <span class="journal-volume">8</span> <span class="journal-issue">10</span> <span class="page"> Paper e1000522. 4 p. </span> <span class="year">(2010)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_GOLD" title=" Gold "> <a style="color:black" title="10.1371/journal.pbio.1000522" target="_blank" href="https://doi.org/10.1371/journal.pbio.1000522"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000283495100019" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000283495100019"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="79952277141" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-79952277141"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="21048991" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=21048991&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Agricultural and Biological Sciences (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Biochemistry, Genetics and Molecular Biology (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Immunology and Microbiology (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Neuroscience (miscellaneous)&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;D1</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 171</span> | Független: 147 | Függő: 24 | Nem jelölt: 0 | WoS jelölt: 167 | Scopus jelölt:&nbsp;110 | WoS/Scopus jelölt:&nbsp;171 | DOI jelölt:&nbsp;170 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;1498567&sort=publishedYear,desc&sort=title"> Idézett közlemények száma: 1 </a> </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 1498567</span> | <span class="status-data status-ADMIN_APPROVED"> Admin láttamozott </span> <span class="oldId">Régi azonosító: 1498567</span> | Forrás Idéző | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">WOS</span> </div> <div class="lastModified">Utolsó módosítás: 2022.09.07. 12:22 Vándor Andrea (VA, admin) </div> <div class="lockedBy">Központi kezelésű 2022.09.08. 08:43 Vándor Andrea (VA, admin) </div> </div></div>