https://m2.mtmt.hu/ hun 2026-05-01 15:36 JournalArticle 1498566 APPROVED true 0 2014-10-16T16:19:10.000+0000 false 1498566 2025-12-20T23:26:12.787+0000 2014-10-16T16:19:10.000+0000 2011-04-11T17:18:01.000+0000 true 10021050 Csúcs Gábor /api/author/10021050 Author true 10021050 2018-10-20T16:22:38.653+0000 2018-10-20T16:22:38.653+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Kozak, K Workflow-Based Software Environment for Large-Scale Biological Experiments true true /api/journal/23785 REVIEWED true false 23785 false 23785 true 1087-0571 1552-454X Journal FOREIGN 15 7 892 899 892 8 2010 High-content screening (HCS) technologies are becoming increasingly used in both large-scale drug discovery and basic research programs. These automated imaging and analysis technologies enable the researcher to elucidate the complex biology that underlies the functions of genes, proteins, and other biomolecules at the cellular level. HCS combines the power of automated digital microscopy and advanced software-based image analysis algorithms to detect and quantify biological changes in cells and tissues. This technology is a particularly powerful tool when used to interrogate the cellular effects of exogenously applied agents such as RNAi and/or small molecules. HCS allows for the evaluation of cellular perturbations that occur both at the level of the single cell and within cellular populations. In a multivariate approach, multiple cellular parameters are collected, allowing for more complex analysis. However, in these scenarios, data flow and management still represent substantial bottlenecks in HCS projects. HCS data include a diversity of information from multiple sources such as details pertaining to screening libraries (e.g., siRNA and small molecules), image stacks acquired from automated microscopes (of which there may be up to several million), and the image analysis data. From this, postprocessing algorithms are required to generate statistical, quality control bioinformatic information and ultimately a final hit list. To accomplish these individual tasks, numerous tools can be used to perform each analytical step; however, management of the entire information flow currently requires the use of commercially available proprietary software, the scope of which is often limited, or bespoke customized scripts. In this article, the authors introduce an open-source research tool that allows for the management of the entire data flow of the HCS data chain, by handling and linking information and providing many powerful postprocessing and visualization tools. 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<span class="author-name" >Bakos G </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Hoff A </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Bennett E </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Dunican D </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Davies A </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Kelleher D </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Long A </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10021050"><a href="/gui2/?type=authors&mode=browse&sel=10021050" target="_blank">Csucs G (<span class="authorship-author-name">Csúcs Gábor</span> <span class="authorAux-mtmt"> Biofizika, sejtbiológia, nanotechnológia</span>) </a> </span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;1498566" target="_blank">Workflow-Based Software Environment for Large-Scale Biological Experiments</a></div> <div> <span class="journal-title">JOURNAL OF BIOMOLECULAR SCREENING</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/1087-0571">1087-0571</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1552-454X">1552-454X</a>)</span>: <span class="journal-volume">15</span> <span class="journal-issue">7</span> <span class="page"> pp 892-899 </span> <span class="year">(2010)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Nyelv: Angol | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:black" title="10.1177/1087057110377354" target="_blank" href="https://doi.org/10.1177/1087057110377354"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000283799100018" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000283799100018"> WoS </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Folyóirat szakterülete: Scopus - Drug Discovery&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q1</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Analytical Chemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Biochemistry&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Biotechnology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Molecular Medicine&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> <div class="journal-subject">Folyóirat szakterülete: Scopus - Pharmacology&nbsp;&nbsp;&nbsp;SJR indikátor:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="Nyilvános idézőközlemények összesen, említések nélkül" class="citingPub-count">Nyilvános idéző összesen: 13</span> | Független: 10 | Függő: 3 | Nem jelölt: 0 | WoS jelölt: 13 | Scopus jelölt:&nbsp;4 | WoS/Scopus jelölt:&nbsp;13 | DOI jelölt:&nbsp;12 </div> <div class="mtid"><span class="long-pub-mtid">Közlemény: 1498566</span> | <span class="status-data status-APPROVED"> Nyilvános </span> <span class="oldId">Régi azonosító: 1498566</span> | Forrás | <span class="type-subtype">Folyóiratcikk ( Szakcikk ) </span> | <span class="pub-category">Tudományos</span> | <span class="publication-sourceOfData">WOS</span> </div> <div class="lastModified">Utolsó módosítás: 2014.10.16. 18:19 MTMT Admin (admin) </div> </div></div>