Impaired regulatory T-cell homeostasis due to vitamin D deficiency in undifferentiated connective tissue disease

Zold, E [Zöld, Éva (belgyógyászat, im...), szerző] C épület (DE / KK / BelgyKL); Szodoray, P; Kappelmayer, J [Kappelmayer, János (Haemostasis, leuk...), szerző] Laboratóriumi Medicina Intézet (DE / ÁOK); Gaal, J [Gaál, János (belgyógyászat, re...), szerző]; Csathy, L [Csáthy, László (Áramlási citometria), szerző] Laboratóriumi Medicina Intézet (DE / ÁOK); Barath, S [Baráth, Sándor (immunológia, klin...), szerző] C épület (DE / KK / BelgyKL); Gyimesi, E [Gyimesi, Edit (Immunológia), szerző]; Hajas, A [Hajas, Ágota (orvostudományok), szerző]; Zeher, M [Zeher, Margit (Belgyógyászat, kl...), szerző] C épület (DE / KK / BelgyKL); Szegedi, G [Szegedi, Gyula (Belgyógyászat), szerző] C épület (DE / KK / BelgyKL); Bodolay, E [Bodolay, Edit (Immunológia), szerző] C épület (DE / KK / BelgyKL)

Angol nyelvű Szakcikk (Folyóiratcikk) Tudományos
  • SJR Scopus - Medicine (miscellaneous): Q1
Azonosítók
Szakterületek:
  • Általános orvostudomány
  • Biológiai tudományok
  • Egyéb orvostudományok
  • Klinikai orvostan
Objective: The aim of this study was to perform a quantitative and functional analysis of natural CD4+CD25highFoxp3+ regulatory T cells (nTregs) and CD4+IL-17+ T cells, and to assess the serum levels of proinflammatory cytokines in patients with undifferentiated connective tissue disease (UCTD) before and after 5 weeks of 0.5 μg/day alfacalcidol supplementation. Methods: Twenty-five patients with UCTD were enrolled in an open-label trial of alfacalcidol. Plasma levels of 25-hydroxyvitamin D [25(OH)D] were assessed by a high-performance liquid chromatography (HPLC) method. Flow cytometry was used for the quantification of nTregs and the IL-17 expression of T-helper (Th)17 cells. The serum concentrations of cytokines interleukin (IL)-12, interferon (IFN)-γ, IL-23, IL-17, IL-6, and IL-10 were measured by an enzyme-linked immunosorbent assay (ELISA). Results: Treatment with alfacalcidol raised 25(OH)D levels from a mean of 23.5 ± 5.6 to 34.5 ± 7.4 ng/mL (p = 0.059; NS). Alfacalcidol treatment decreased both Th1- (IL-12 and IFN-γ) and Th17-related (IL-23, IL-17, IL-6) cytokine levels in UCTD patients, while the soluble IL-10 level increased (IL-12: 156.7 ± 75.2 vs. 87.5 ± 42.1 pg/mL, p < 0.001; IFN-γ: 41.5 ± 12.0 vs. 21.7 ± 9.9 pg/mL, p < 0.001; IL-23: 385.2 ± 82.2 vs. 210.0 ± 69.3 pg/mL, p < 0.001; IL-17: 37.8 ± 9.6 vs. 17.8 ± 4.5 pg/mL, p 0.009; IL-6: 39.4 ± 11.3 vs. 23.5 ± 6.3 pg/mL, p < 0.001, IL-10: 8.4 ± 3.0 vs. 21.4 ± 9.7 pg/mL, p < 0.001). Alfacalcidol improved the Th17nTreg imbalance, as it inhibited the IL-17 expression of Th17 cells, and increased the number of nTregs. The alfacalcidol might increase the capacity of nTreg cells to suppress the proliferation of autologous CD4+CD25- cells. Conclusion: Our findings support the idea that vitamin D influences the Th17nTreg imbalance in vitamin D-insufficient patients with UCTD and could be beneficial in the management of the disease. © 2010 Taylor & Francis on license from Scandinavian Rheumatology Research Foundation.
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2025-02-10 16:40