We propose a two-photon microscope scheme capable of real-time, three-dimensional
investigation of the electric activity pattern of neural networks or signal summation
rules of individual neurons in a 0.6 mm X 0.6 mm X 0.2 mm volume of the sample. The
points of measurement are chosen according to a conventional scanning two-photon image,
and they are addressed by separately adjustable optical fibers. This allows scanning
at kilohertz repetition rates of as many as 100 data points. Submicrometer spatial
resolution is maintained during the measurement similarly to conventional two-photon
microscopy. (c) 2007 Optical Society of America.
