Endothelial function and vascular oxidative stress in long-lived GH/IGF-deficient Ames dwarf mice

Csiszar, A [Csiszar, Anna (Orvostudomany), szerző]; Labinskyy, N; Perez, V; Recchia, FA; Podlutsky, A; Mukhopadhyay, P; Losonczy, G [Losonczy, György (Pulmonológia), szerző] Pulmonológiai Klinika (SE / AOK / K); Pacher, P [Pacher, Pál (Kardiovaszkuláris...), szerző]; Austad, SN; Bartke, A; Ungvari, Z ✉ [Ungvári, Zoltán István (Orvostudomány, él...), szerző] Pulmonológiai Klinika (SE / AOK / K)

Angol nyelvű Tudományos Szakcikk (Folyóiratcikk)
  • SJR Scopus - Cardiology and Cardiovascular Medicine: D1
Azonosítók
Szakterületek:
    Csiszar A, Labinskyy N, Perez V, Recchia FA, Podlutsky A, Mukhopadhyay P, Losonczy G, Pacher P, Austad SN, Bartke A, Ungvari Z. Endothelial function and vascular oxidative stress in long-lived GH/IGF-deficient Ames dwarf mice. Am J Physiol Heart Circ Physiol 295: H1882-H1894, 2008. First published August 29, 2008; doi:10.1152/ajpheart.412.2008. - Hypopituitary Ames dwarf mice have low circulating growth hormone (GH)/IGF-I levels, and they have extended longevity and exhibit many symptoms of delayed aging. To elucidate the vascular consequences of Ames dwarfism we compared endothelial O-2(center dot-) and H2O2 production, mitochondrial reactive oxygen species (ROS) generation, expression of antioxidant enzymes, and nitric oxide (NO) production in aortas of Ames dwarf and wild-type control mice. In Ames dwarf aortas endothelial O-2(center dot-) and H2O2 production and ROS generation by mitochondria were enhanced compared with those in vessels of wild-type mice. In Ames dwarf aortas there was a less abundant expression of Mn-SOD, Cu, Zn-SOD, glutathione peroxidase (GPx)-1, and endothelial nitric oxide synthase (eNOS). NO production and acetylcholine-induced relaxation were also decreased in aortas of Ames dwarf mice. In cultured wild-type mouse aortas and in human coronary arterial endothelial cells treatment with GH and IGF significantly reduced cellular O-2(center dot-) and H2O2 production and ROS generation by mitochondria and upregulated expression of Mn-SOD, Cu, Zn-SOD, GPx-1, and eNOS. Thus GH and IGF-I promote antioxidant phenotypic changes in the endothelial cells, whereas Ames dwarfism leads to vascular oxidative stress.
    Hivatkozás stílusok: IEEEACMAPAChicagoHarvardCSLMásolásNyomtatás
    2021-04-21 04:52