Influence of hyperthyroidism on the effect of adenosine transport blockade assessed by a novel method in guinea pig atria

Karsai, D; Gesztelyi, R [Gesztelyi, Rudolf (Általános farmako...), author] Faculty of Pharmacy (UD); Zsuga, J [Zsuga, Judit (orvostudomány), author] Neurológiai Klinika (UD / ÁOK); Jakab, A; Szendrei, L; Juhasz, B [Juhász, Béla (Kardiovaszkuláris...), author] Faculty of Pharmacy (UD); Bak, I [Bak, István (Kardiovaszkuláris...), author] Faculty of Pharmacy (UD); Szabo, G; Lekli, I [Lekli, István (Kísérletes farmak...), author] Gyógyszerhatástani Tanszék (UD); Vecsernyes, M [Vecsernyés, Miklós (gyógyszerésztudomány), author] Faculty of Pharmacy (UD); Varga, E; Szentmiklosi, AJ [Szentmiklósi, József András (Elméleti orvostud...), author]; Tosaki, A [Tósaki, Árpád (Kardiovaszkuláris...), author] Gyógyszerhatástani Tanszék (UD)

English Scientific Article (Journal Article)
  • SJR Scopus - Medicine (miscellaneous): Q1
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    The aim of the present study was to investigate the effect of hyperthyroidism on the trans-sarcolemmal adenosine (Ado) flux via equilibrative and nitrobenzylthioinosine (NBTI)-sensitive nucleoside transporters (ENT1) in guinea pig atria, by assessing the change in the Ado concentration of the interstitial fluid ([Ado](ISF)) under nucleoside transport blockade with NBTL For the assessment, we applied our novel method, which estimates the change in [Ado](ISF) utilizing the altered inotropic response to N-6-cyclopentyladenosine (CPA), a relative stable selective agonist of A(1) Ado receptors, by providing a relative index, the equivalent concentration of CPA. Our results show an interstitial Ado accumulation upon ENT1 blockade, which was more extensive in the hyperthyroid samples (CPA concentrations equieffective with the surplus [Ado](ISF) were two to three times higher in hyperthyroid atria than in euthyroid ones, with regard to the negative inotropic effect of CPA and Ado). This suggests an enhanced Ado influx via ENT1 in hyperthyroid atria. It is concluded that hyperthyroidism does not alter the prevailing direction of the Ado transport, moreover intensifies the Ado influx in the guinea pig atrium.
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    2020-09-30 21:37