Chitosan microparticles have been prepared by emulsion cross-linking method. Glutaraldehyde
was used as cross-linking agent, which allows covalent attachment of the enzyme.
A wide range of particle size could be produced using the emulsion cross-linking method.
Practically, any desired size between 20 and 500 μm could be reached by properly choosing
type and concentration of surfactant. The obtained microparticles proved to be suitable
for immobilization of β-galactosidase. β-Galactosidase enzyme was immobilized onto
microparticles to obtain solid-phase biocatalyst. The
biocatalysts fixed on microspheres were stable at 4ºC for 3. The immobilized β-galactosidase
proved to be very stable and operated with almost the same yields throughout the performed