https://m2.mtmt.hu/ eng 2026-05-01 23:49 JournalArticle 109677 APPROVED true Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony utca 43, H-1083 Budapest, Hungary Department of Anatomy, Hokkaido University School of Medicine, Sapporo, 060-8638, Japan Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN 47405, United States Department of Molecular Psychiatry, Life and Brain Center, University of Bonn, 53105 Bonn, Germany Cited By :144 Export Date: 8 June 2023 CODEN: NEPHB Correspondence Address: Katona, I.; Institute of Experimental Medicine, Szigony utca 43, H-1083 Budapest, Hungary; email: katona@koki.hu Chemicals/CAS: lipoprotein lipase, 83137-80-8, 9004-02-8; tyrosine 3 monooxygenase, 9036-22-0; 2-arachidonylglycerol, 53847-30-6; Arachidonic Acids; Endocannabinoids; Galactolipids; Glutamic Acid, 56-86-0; Glycerides; Receptor, Cannabinoid, CB1; Tyrosine 3-Monooxygenase, 1.14.16.2; diacylgalactosylglycerol; gamma-Aminobutyric Acid, 56-12-2 Funding details: 1A/002/2004 Funding details: National Institutes of Health, NIH Funding details: Howard Hughes Medical Institute, HHMI Funding details: National Institute on Drug Abuse, NIDA, K02DA000286, K05DA021696, R01DA011322 Funding details: National Institute of Neurological Disorders and Stroke, NINDS, R01NS030549 Funding details: Egészségügyi Tudományos Tanács, ETT, 561/2006 Funding details: European Commission, EC, LSHM-CT-2004-005166 Funding details: Hungarian Scientific Research Fund, OTKA, F046407, T046820 Funding text 1: This work was supported by the Howard Hughes Medical Institute (T.F.F.), Országos Tudományos Kutatási Alapprogramok (OTKA) Grants T046820 (T.F.F.) and F046407 (I.K.), Egészségügyi Tudományos Tanács (ETT 561/2006) (I.K.), Nemzeti Kutatási és Fejlesztési Pályázatok (NKFP) Grant 1A/002/2004 (T.F.F.), National Institutes of Health Grants DA00286 and DA11322 (K.M.), NS30549 (T.F.F) and European Union Contract LSHM-CT-2004-005166 (T.F.F. and A.Z.). I.K. is a grantee of the János Bolyai scholarship. We are grateful to Katalin Lengyel, Emöke Simon, Katalin Iványi and Gyözö Goda for excellent technical assistance and to Rita Nyilas for help with brain samples. 0 2017-02-22T09:05:44.000+0000 false 109677 2026-04-22T10:32:04.946+0000 2023-03-21T10:40:52.806+0000 2010-06-13T18:16:49.000+0000 true 10003154 Wolf György /api/admin/10003154 Admin György Wolf (KOKI admin) true 10003154 2025-04-16T13:30:02.169+0000 2025-04-16T13:30:02.169+0000 true false true 24 24 /api/publicationtype/24 PublicationType Journal Article 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType Journal Article 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType Article (Journal Article) 101 true 10000059 true 1 /api/category/1 Category Scientific true 1 Mátyás, F true true /api/journal/3361 REVIEWED NEUROPHARMACOLOGY 0028-3908 1873-7064 true false 3361 false 3361 true 0028-3908 1873-7064 Journal FOREIGN 54 95 107 95 13 2008 Intact endogenous cannabinoid signaling is involved in several aspects of drug addiction. Most importantly, endocannabinoids exert pronounced influence on primary rewarding effects of abused drugs, including exogenous cannabis itself, through the regulation of drug-induced increase in bursting activity of dopaminergic neurons in the ventral tegmental area (VTA). Previous electrophysiological studies have proposed that these dopaminergic neurons may release endocannabinoids in an activity-dependent manner to regulate their various synaptic inputs; however, the underlying molecular and anatomical substrates have so far been elusive. To facilitate understanding of the neurobiological mechanisms involving endocannabinoid signaling in drug addiction, we carried out detailed analysis of the molecular architecture of the endocannabinoid system in the VTA. In situ hybridization for sn-1-diacylglycerol lipase-alpha (DGL-alpha), the biosynthetic enzyme of the most abundant endocannabinoid, 2-arachidonoylglycerol (2-AG), revealed that DGL-alpha was expressed at moderate to high levels by most neurons of the VTA. Immunostaining for DGL-alpha resulted in a widespread punctate pattern at the light microscopic level, whereas high-resolution electron microscopic analysis demonstrated that this pattern is due to accumulation of the enzyme adjacent to postsynaptic specializations of several distinct morphological types of glutamatergic and GABAergic synapses. These axon terminal types carried presynaptic CB(1) cannabinoid receptors on the opposite side of DGL-alpha-containing synapses and double immunostaining confirmed that DGL-alpha is present on the plasma membrane of both tyrosine hydroxylase (TH)-positive (dopaminergic) and TH-negative dendrites. These findings indicate that retrograde synaptic signaling mediated by 2-AG via CB(1) may influence the drug-reward circuitry at multiple types of synapses in the VTA. true true 2010 true true true false true false NONE 2026-04-22 false 158 0 158 145 8 156 155 145 155 158 158 145 145 145 13 0 158 145 158 145 0 7 7 Folyóiratcikk Journal Article 24 141 Könyvrészlet Chapter in Book 25 17 Könyv Book 23 0 Egyéb konferenciaközlemény Conference paper 31 0 Egyéb konferenciakötet Conference proceedings 32 0 Oltalmi formák Protection forms 26 0 Disszertáció Thesis 28 0 Egyéb Miscellaneous 29 0 Alkotás Achievement 22 0 Kutatási adat Research data 33 0 Folyóiratcikk Journal Article 24 0 0 0 Könyvrészlet Chapter in Book 25 0 0 0 Könyv Book 23 0 0 0 Egyéb konferenciaközlemény Conference paper 31 0 0 0 Egyéb konferenciakötet Conference proceedings 32 0 0 0 Oltalmi formák Protection forms 26 0 0 0 Disszertáció Thesis 28 0 0 0 Egyéb Miscellaneous 29 0 0 0 Alkotás Achievement 22 0 0 0 Kutatási adat Research data 33 0 0 0 2008 0 7 6 7 6 2009 0 5 2 5 2 2010 0 12 11 12 11 2011 0 9 8 9 8 2012 0 19 15 19 15 2013 0 7 7 7 7 2014 0 5 5 5 5 2015 0 18 17 18 17 2016 0 13 12 13 12 2017 0 7 7 7 7 2018 0 7 7 7 7 2019 0 5 4 5 4 2020 0 5 5 5 5 2021 0 12 12 12 12 2022 0 7 7 7 7 2023 0 12 12 12 12 2024 0 4 4 4 4 2025 0 4 4 4 4 Q1 false false false false 2013-02-13T13:16:02.000+0000 2013-02-13T13:13:15.000+0000 Agykéreg Kutatócsoport (KOKI / CHO); MTA Kísérleti Orvostudományi Kutatóintézet; Thalamus Kutatócsoport (HRN KOKI) 4 40 3 7 0 true Language 10002 /api/language/10002 English Angol English true 2 true PersonAuthorship 90229 /api/authorship/90229 Mátyás, F [Mátyás, Ferenc (Neurobiológia), author] Laboratory of Thalamus Research 1 0.14285715 true false Author 163 /api/author/163 Ferenc Mátyás (Neurobiológia) Mátyás Ferenc true 163 true Mátyás F true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false 17341 true PersonAuthorship 90230 /api/authorship/90230 Urbán, GM [Katona-Urbán, Gabriella (Molekuláris neuro...), author] MTA Kísérleti Orvostudományi Kutatóintézet 2 0.143 false false Author 164 /api/author/164 Gabriella Katona-Urbán (Molekuláris neurobiológia) Katona-Urbán Gabriella true 164 true Urbán GM true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false 17340 true PersonAuthorship 90231 /api/authorship/90231 Watanabe, M 3 0.14285715 false false Watanabe M true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false 17339 true PersonAuthorship 90232 /api/authorship/90232 Mackie, K 4 0.14285715 false false Mackie K true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false 17338 true PersonAuthorship 90233 /api/authorship/90233 Zimmer, A 5 0.14285715 false false Zimmer A true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false 17337 true PersonAuthorship 90234 /api/authorship/90234 Freund, TF [Freund, Tamás (Neurobiológia, id...), author] Laboratory of Cerebral Cortex Research (IEM / DCNN) 6 0.14285715 false false Author 10000275 /api/author/10000275 Tamás Freund (Neurobiológia, idegtudomány) Freund Tamás true 10000275 true Freund TF true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false 17336 true PersonAuthorship 90235 /api/authorship/90235 Katona, I ✉ [Katona, István (Neurobiológia), author] MTA Kísérleti Orvostudományi Kutatóintézet 7 0.143 false true true Author 10011502 /api/author/10011502 István Katona (Neurobiológia) Katona István true 10011502 true Katona I true false false AuthorshipType 1 /api/authorshiptype/1 Author 0 true 0 true false 17335 true PublicationIdentifier 36512 /api/publicationidentifier/36512 DOI: 10.1016/j.neuropharm.2007.05.028 PlainSource 6 /api/publicationsource/6 DOI PublicationSourceType 10001 /api/publicationsourcetype/10001 DOI true true true DOI DOI https://doi.org/@@@ true true 6 true false IDENTICAL 10.1016/j.neuropharm.2007.05.028 https://doi.org/10.1016/j.neuropharm.2007.05.028 false 11252 true PublicationIdentifier 36510 /api/publicationidentifier/36510 WoS: 000252560300013 PlainSource 1 /api/publicationsource/1 WoS PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true WoS WoS https://www.webofscience.com/wos/woscc/full-record/@@@ true true 1 true false IDENTICAL 000252560300013 https://www.webofscience.com/wos/woscc/full-record/000252560300013 false 11253 true PublicationIdentifier 36511 /api/publicationidentifier/36511 Scopus: 37349043211 PlainSource 3 /api/publicationsource/3 Scopus PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true Scopus Scopus http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@ true true 3 true false IDENTICAL 37349043211 http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-37349043211 false 1401046 true PublicationIdentifier 36513 /api/publicationidentifier/36513 PubMed: 17655884 PlainSource 17 /api/publicationsource/17 PubMed PublicationSourceType 10003 /api/publicationsourcetype/10003 Indexelő adatbázis false true true PubMed PubMed http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract true true 17 true false NO 17655884 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17655884&dopt=Abstract false 11254 true Classification 12125 /api/classification/12125 Basic medicine true true Classification 10545 /api/classification/10545 Biological sciences true true SjrRating 5858885 /api/sjrrating/5858885 sjr:Q1 (2008) Scopus - Pharmacology NEUROPHARMACOLOGY 0028-3908 1873-7064 34 0.24 journal RatingType 10002 /api/ratingtype/10002 sjr sjr true true ClassificationExternal 3004 /api/classificationexternal/3004 Scopus - Pharmacology true 3004 true Q1 DIRECT true 5858885 true /api/publication/109677 Mátyás F et al. Identification of the sites of 2-arachidonoylglycerol synthesis and action imply retrograde endocannabinoid signaling at both gabaergic and glutamatergic synapses in the ventral tegmental area. (2008) NEUROPHARMACOLOGY 0028-3908 1873-7064 54 95-107<div class="JournalArticle Publication long-list"> <div class="authors"> <img title="Forrásközlemény" style="float: left" src="/frontend/resources/grid/publication-core-icon.png"> <img title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" mtid="163"><a href="/gui2/?type=authors&mode=browse&sel=163" target="_blank">Mátyás F (<span class="authorship-author-name">Mátyás Ferenc</span> <span class="authorAux-mtmt"> Neurobiológia</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Cellular and Network Neurobiology">DCNN</span>/Laboratory of Thalamus Research</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="164"><a href="/gui2/?type=authors&mode=browse&sel=164" target="_blank">Urbán GM (<span class="authorship-author-name">Katona-Urbán Gabriella</span> <span class="authorAux-mtmt"> Molekuláris neurobiológia</span>) </a> </span> <span class="author-affil">MTA Kísérleti Orvostudományi Kutatóintézet</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Watanabe M </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Mackie K </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Zimmer A </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000275"><a href="/gui2/?type=authors&mode=browse&sel=10000275" target="_blank">Freund TF (<span class="authorship-author-name">Freund Tamás</span> <span class="authorAux-mtmt"> Neurobiológia, idegtudomány</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Cellular and Network Neurobiology">DCNN</span>/Laboratory of Cerebral Cortex Research</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10011502"><a href="/gui2/?type=authors&mode=browse&sel=10011502" target="_blank">Katona I ✉ (<span class="authorship-author-name">Katona István</span> <span class="authorAux-mtmt"> Neurobiológia</span>) </a> </span> <span class="author-affil">MTA Kísérleti Orvostudományi Kutatóintézet</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;109677" target="_blank">Identification of the sites of 2-arachidonoylglycerol synthesis and action imply retrograde endocannabinoid signaling at both gabaergic and glutamatergic synapses in the ventral tegmental area</a></div> <div> <span class="journal-title">NEUROPHARMACOLOGY</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0028-3908">0028-3908</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1873-7064">1873-7064</a>)</span>: <span class="journal-volume">54</span> <span class="page"> pp 95-107 </span> <span class="year">(2008)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.1016/j.neuropharm.2007.05.028" target="_blank" href="https://doi.org/10.1016/j.neuropharm.2007.05.028"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="000252560300013" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/000252560300013"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="37349043211" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-37349043211"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="17655884" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17655884&dopt=Abstract"> PubMed </a> </span> </span> <OnlyViewableByAuthor><div class="ratings"> <div class="journal-subject">Journal subject: Scopus - Pharmacology&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q1</div> <div class="journal-subject">Journal subject: Scopus - Cellular and Molecular Neuroscience&nbsp;&nbsp;&nbsp;Rank:&nbsp;Q2</div> </div></OnlyViewableByAuthor> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 158</span> | Independent citation: 145 | Self citation: 13 | Unknown citation: 0 | Number of citations in WoS: 145 | Number of citations in Scopus:&nbsp;155 | WoS/Scopus assigned:&nbsp;158 | Number of citations with DOI:&nbsp;155 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;109677&sort=publishedYear,desc&sort=title"> Number of cited publications: 7 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 109677</span> | <span class="status-data status-APPROVED"> Published </span> <span class="oldId">Old id: 109677</span> | Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">MEDLINE</span> </div> <div class="lastModified">Last Modified: 2023.03.21. 11:40 Márta Turek (KOKI admin 4) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Comments</u>: Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony utca 43, H-1083 Budapest, Hungary Department of Anatomy, Hokkaido University School of Medicine, Sapporo, 060-8638, Japan Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN 47405, United States Department of Molecular...</pre> </div></div>