https://m2.mtmt.hu/ eng 2026-10-25 06:52 JournalArticle 107660 APPROVED true Department of Anaesthesiology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, 10467, United States Department of Pharmacology, Institute of Experimental Medicine, Hungarian Academy of Sciences, P.O. Box 67, Budapest, H-1450, Hungary Cited By :30 Export Date: 12 August 2019 Correspondence Address: Nagano, O.; Department of Anaesthesiology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, 10467, United States Chemicals/CAS: 2 chloroadenosine, 146-77-0; 8 cyclopentyl 1,3 dipropylxanthine, 102146-07-6; 8 phenyltheophylline, 961-45-5; acetylcholine, 51-84-3, 60-31-1, 66-23-9; adenosine 5' (n ethylcarboxamide), 35920-39-9; adenosine, 58-61-7; dipyridamole, 58-32-2; magnesium, 7439-95-4; theophylline, 58-55-9, 5967-84-0, 8055-07-0, 8061-56-1, 99007-19-9; tritium, 10028-17-8; tubocurarine chloride, 57-94-3, 57-95-4, 8006-51-7; 6 n cyclopentyladenosine, 41552-82-3; choline, 123-41-1, 13232-47-8, 1927-06-6, 4858-96-2, 62-49-7, 67-48-1; hemicholinium 3, 16478-59-4; 1,3-dipropyl-8-cyclopentylxanthine, 102146-07-6; 2-Chloroadenosine, 146-77-0; 8-phenyltheophylline, 961-45-5; Acetylcholine, 51-84-3; Adenosine, 58-61-7; Adenosine-5'-(N-ethylcarboxamide), 35920-39-9; Dipyridamole, 58-32-2; Magnesium, 7439-95-4; N(6)-cyclopentyladenosine, 41552-82-3; Receptors, Purinergic; Theophylline, 58-55-9; Tritium, 10028-17-8; Tubocurarine, 57-95-4; Xanthines Manufacturers: rbi, United States; sigma, United States Department of Anaesthesiology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, 10467, United States Department of Pharmacology, Institute of Experimental Medicine, Hungarian Academy of Sciences, P.O. Box 67, Budapest, H-1450, Hungary Cited By :30 Export Date: 20 April 2020 Correspondence Address: Nagano, O.; Department of Anaesthesiology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, 10467, United States Chemicals/CAS: 2 chloroadenosine, 146-77-0; 6 n cyclopentyladenosine, 41552-82-3; 8 cyclopentyl 1,3 dipropylxanthine, 102146-07-6; 8 phenyltheophylline, 961-45-5; acetylcholine, 51-84-3, 60-31-1, 66-23-9; adenosine 5' (n ethylcarboxamide), 35920-39-9; adenosine, 58-61-7; choline, 123-41-1, 13232-47-8, 1927-06-6, 4858-96-2, 62-49-7, 67-48-1; dipyridamole, 58-32-2; hemicholinium 3, 16478-59-4; tubocurarine chloride, 57-94-3, 57-95-4, 8006-51-7; magnesium, 7439-95-4; theophylline, 58-55-9, 5967-84-0, 8055-07-0, 8061-56-1, 99007-19-9; tritium, 10028-17-8; 1,3-dipropyl-8-cyclopentylxanthine, 102146-07-6; 2-Chloroadenosine, 146-77-0; 8-phenyltheophylline, 961-45-5; Acetylcholine, 51-84-3; Adenosine, 58-61-7; Adenosine-5'-(N-ethylcarboxamide), 35920-39-9; Dipyridamole, 58-32-2; Magnesium, 7439-95-4; N(6)-cyclopentyladenosine, 41552-82-3; Receptors, Purinergic; Theophylline, 58-55-9; Tritium, 10028-17-8; Tubocurarine, 57-95-4; Xanthines Manufacturers: rbi, United States; sigma, United States Department of Anaesthesiology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, 10467, United States Department of Pharmacology, Institute of Experimental Medicine, Hungarian Academy of Sciences, P.O. Box 67, Budapest, H-1450, Hungary Cited By :30 Export Date: 21 April 2020 Correspondence Address: Nagano, O.; Department of Anaesthesiology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, 10467, United States Chemicals/CAS: 2 chloroadenosine, 146-77-0; 6 n cyclopentyladenosine, 41552-82-3; 8 cyclopentyl 1,3 dipropylxanthine, 102146-07-6; 8 phenyltheophylline, 961-45-5; acetylcholine, 51-84-3, 60-31-1, 66-23-9; adenosine 5' (n ethylcarboxamide), 35920-39-9; adenosine, 58-61-7; choline, 123-41-1, 13232-47-8, 1927-06-6, 4858-96-2, 62-49-7, 67-48-1; dipyridamole, 58-32-2; hemicholinium 3, 16478-59-4; tubocurarine chloride, 57-94-3, 57-95-4, 8006-51-7; magnesium, 7439-95-4; theophylline, 58-55-9, 5967-84-0, 8055-07-0, 8061-56-1, 99007-19-9; tritium, 10028-17-8; 1,3-dipropyl-8-cyclopentylxanthine, 102146-07-6; 2-Chloroadenosine, 146-77-0; 8-phenyltheophylline, 961-45-5; Acetylcholine, 51-84-3; Adenosine, 58-61-7; Adenosine-5'-(N-ethylcarboxamide), 35920-39-9; Dipyridamole, 58-32-2; Magnesium, 7439-95-4; N(6)-cyclopentyladenosine, 41552-82-3; Receptors, Purinergic; Theophylline, 58-55-9; Tritium, 10028-17-8; Tubocurarine, 57-95-4; Xanthines Manufacturers: rbi, United States; sigma, United States 0 2018-09-12T12:19:44.000+0000 false 107660 2026-10-12T19:00:21.475+0000 2024-08-29T07:38:46.897+0000 2010-06-13T01:45:03.000+0000 true 10003154 Wolf György /api/admin/10003154 Admin true 10003154 2026-01-12T09:23:05.932+0000 2026-01-12T09:23:05.932+0000 Admin 10066925 /api/admin/10066925 Nemec Évi true true Admin 10067876 /api/admin/10067876 Ladányi Gusztáv true true 2024-10-17T16:20:19.023+0000 true false true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle true 10000059 Article true 24 24 /api/publicationtype/24 PublicationType 1 true 24 JournalArticle /api/subtype/10000059 Szakcikk SubType 101 true 10000059 true 1 /api/category/1 Category true 1 Nagano, O Presynaptic A₁-purinoceptor-mediated inhibitory effects of adenosine and its stable analogues on the mouse hemidiaphragm preparation true true /api/journal/3297 REVIEWED true false 3297 false 3297 true 0028-1298 1432-1912 Journal FOREIGN 346 2 197 202 197 6 1992 The effect of adenosine or its stable analogues (2-chloroadenosine, CADO; 5\\t'-N-ethylcarboxamidoadenosine, NECA; and N6-cyclopentyladenosine, CPA) on the release of [3H]-acetylcholine ([3H]-ACh), and on the development of force of contraction evoked by electrical stimulation of the nerve, were studied in the mouse phrenic nerve-hemidiaphragm preparation. Evidence was obtained that the release of ACh is subject to presynaptic modulation through presynaptic A1(P1)-purinoceptors. Adenosine or its stable analogues (CADO, NECA, CPA) inhibited, in a concentration-dependent manner, both the release of ACh and the force of the indirectly elicited contraction of hemidiaphragm preparation, provided in the latter case that the margin of safety was reduced by (\\s+)-tubocurarine or magnesium. The order of potency in reducing ACh release was CPA > NECA > CADO > adenosine with IC50 values of 0.08 \\+- 0.01, 0.74 \\+- 0.05, 9.05 \\+- 0.20, and 410.2 \\+- 42.5 \\gmmol/l, respectively. The order of potency in reducing twitch tension was CPA > NECA > CADO > adenosine with IC50 values of 0.11 \\+- 0.02, 0.48 \\+- 0.03, 2.07 \\+- 0.49, and 240.4 \\+- 20.0 \\gmol/l, respectively. 8-Phenyltheophylline (8-PT) antagonized the inhibitory effects of the adenosine receptor agonists on ACh release and twitch tension. The finding that -log KB and pA2 values estimated for 8-PT on ACh release and twitch tension were similar when adenosine or its stable analogues were applied as agonists suggest (i) that the motor nerve terminals are equipped with inhibitory P1-purinoceptors and (ii) that twitch responses of the partially curarized phrenic nerve-hemidiaphragm preparation to electrical stimulation can be used for studying the presynaptic inhibitory effect of P1-purinoceptor agonists. The findings that 8-PT and 8-cyclopentyl-1,3dipropylxanthine (DPCPX), a selective A1-purinoceptor antagonist, enhanced the release of [3H]-ACh in response to nerve stimulation and DPCPX antagonized the inhibitory effect of CADO on twitch tension (pA2 = 10.77 \\+- 1.34) indicate that these receptors are of A1-subtype. Dipyridamole, an adenosine uptake blocker, reduced the release of [3H]-ACh, and reinforced the effect of adenosine, but did not affect responses to adenosine analogues not taken up by the tissue. It is concluded that the cholinergic axon terminals located at the neuromuscular junction are equipped with inhibitory A1-purinoceptors. Endogenous adenosine derived either from ATP or directly from the muscle or nerve terminals might thus activate these receptors to inhibit ACh release and thereby presynaptically influences chemical neurotransmission at the neuromuscular junction. © 1992, Springer-Verlag. 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title="Idézőközlemény" style="float: left" src="/frontend/resources/grid/publication-citation-icon.png"> <div class="autype autype0"> <span class="author-name" >Nagano O </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Földes FF </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Nakatsuka H </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Reich D </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" >Ohta Y </span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10008942"><a href="/gui2/?type=authors&mode=browse&sel=10008942" target="_blank">Sperlagh B (<span class="authorship-author-name">Sperlágh Beáta</span> <span class="authorAux-mtmt"> Neurofarmakológia</span>) </a> </span> <span class="author-affil">MTA Kísérleti Orvostudományi Kutatóintézet; <span title="Semmelweis University">SU</span>/<span title="Faculty of Medicine">FM</span>/<span title="Instituties">I</span>/Department of Pharmacology and Pharmacotherapy</span> ;&nbsp;&nbsp;&nbsp; <span class="author-name" mtid="10000023"><a href="/gui2/?type=authors&mode=browse&sel=10000023" target="_blank">Vizi ES (<span class="authorship-author-name">Vizi E. Szilveszter</span> <span class="authorAux-mtmt"> Neurofarmakológia, neurokémia</span>) </a> </span> <span class="author-affil"><span title="MTA Kísérleti Orvostudományi Kutatóintézet">IEMHAS</span>/<span title="Department of Pharmacology">DP</span>/Laboratory of Drug Research</span> </div> </div> <div class="title"><a href="/gui2/?mode=browse&params=publication;107660" target="_blank">Presynaptic A₁-purinoceptor-mediated inhibitory effects of adenosine and its stable analogues on the mouse hemidiaphragm preparation</a></div> <div> <span class="journal-title">NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY</span> <span class="journal-issn">(<a target="_blank" href="https://portal.issn.org/resource/ISSN/0028-1298">0028-1298</a> <a target="_blank" href="https://portal.issn.org/resource/ISSN/1432-1912">1432-1912</a>)</span>: <span class="journal-volume">346</span> <span class="journal-issue">2</span> <span class="page"> pp 197-202 </span> <span class="year">(1992)</span> </div> <div class="pub-footer"> <span class="language" xmlns="http://www.w3.org/1999/html">Language: English | </span> <span class="identifiers"> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="10.1007/BF00165301" target="_blank" href="https://doi.org/10.1007/BF00165301"> DOI </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="A1992JM89600010" target="_blank" href="https://www.webofscience.com/wos/woscc/full-record/A1992JM89600010"> WoS </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:blue" title="0026616835" target="_blank" href="http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-0026616835"> Scopus </a> </span> <span class="id identifier oa_none" title="none"> <a style="color:black" title="1333058" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=1333058&dopt=Abstract"> PubMed </a> </span> </span> <div class="publication-citation" style="margin-left: 0.5cm;"> <span title="" class="citingPub-count">Citing papers: 37</span> | Independent citation: 30 | Self citation: 7 | Unknown citation: 0 | Number of citations in WoS: 34 | Number of citations in Scopus:&nbsp;35 | WoS/Scopus assigned:&nbsp;37 | Number of citations with DOI:&nbsp;33 </div> <div class="publication-citation"> <a target="_blank" href="/api/publication?cond=citations.related;eq;107660&sort=publishedYear,desc&sort=title"> Number of cited publications: 5 </a> </div> <div class="mtid"><span class="long-pub-mtid">Publication: 107660</span> | <span class="status-data status-APPROVED"> Published </span> <span class="oldId">Old id: 107660</span> | Core Citing | <span class="type-subtype">Journal Article ( Article ) </span> | <span class="pub-category">Scientific</span> | <span class="publication-sourceOfData">kézi felvitel</span> </div> <div class="lastModified">Last Modified: 2024.08.29. 09:38 Márta Turek (KOKI admin 4) </div> <div class="lockedBy">Centrally managed 2024.10.17. 18:20 Évi Nemec (MTMT Központi 3, admin) Gusztáv Ladányi (MTMT API user, admin) </div> <pre class="comment" style="margin-top: 0; margin-bottom: 0;"><u>Comments</u>: Department of Anaesthesiology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, 10467, United States Department of Pharmacology, Institute of Experimental Medicine, Hungarian Academy of Sciences, P.O. Box 67, Budapest, H-1450, Hungary Cited By :30 Export Date: 12 August 2019 Cor...</pre> </div></div>