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The protocol is sensitive enough for electron microscopic localization of endogenous messenger RNAs encoding β- actin and amphoterin. Three visualization methods were compared: diaminobenzidine enhanced by nickel, Nanogold enhanced by silver and gold toning, and fluorescently labeled tyramides. Diaminobenzidine and Nanogold can be used in both light and electron microscopy. The nickel-enhanced diaminobenzidine was the most sensitive visualization method. It is easy to accomplish but a drawback is poor spatial resolution, which restricts its use at high magnifications. Nanogold visualization has considerably better spatial resolution and is therefore recommended for electron microscopy. Fluorescent tyramides, especially TRITC-tyramide, offer a good detection method for fluorescence and confocal microscopy. The methods were used to localize amphoterin and β-actin mRNAs in motile cells. Both mRNAs were found in the soma and cell processes. In double labeling experiments, β-actin mRNA localized to filamentous structures that also contained ribosomal proteins. Especially in the cortical cytoplasm, β-actin mRNA was associated with actin filaments. 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