Idézéskapcsolat

Grkovic Ivana et al. Expression of ecto-nucleoside triphosphate diphosphohydrolase3 (NTPDase3) in the female rat brain during postnatal development. (2016) JOURNAL OF CHEMICAL NEUROANATOMY 0891-0618 1873-6300 77 10-18

Közlemény:
S Kiss David et al. Ecto-nucleoside triphosphate diphosphohydrolase 3 in the ventral and lateral hypothalamic area of female rats: morphological characterization and functional implications. (2009) REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY 1477-7827 7 p. 31
Idéző közlemény:
Grkovic Ivana et al. Expression of ecto-nucleoside triphosphate diphosphohydrolase3 (NTPDase3) in the female rat brain during postnatal development. (2016) JOURNAL OF CHEMICAL NEUROANATOMY 0891-0618 1873-6300 77 10-18
Független: Igen
Megjegyzés: Abstract
Nucleoside triphosphate diphosphohydrolase3 (NTPDase3) is membrane-bound ectoenzyme
which hydrolyzes extracellular ATP, thus modulating the function of purinergic
receptors and the pattern of purinergic signaling. Here we analyzed the developmental
expression of NTPDase3 in female hypothalamus, cerebral cortex and hippocampal formation
at different postnatal ages (PD7–PD90) by qRT-PCR and immunohistochemistry. In
hypothalamus and hippocampus, a similar developmental profile was seen: NTPDase3 gene
expression was stable during postnatal development and increased in adults. In the cortex,
upregulation of NTPDase3 mRNA expression was seen at PD15 and further increase was
evidenced in adults. Immunohistochemical analysis at PD7 revealed faint neuronal NTPDase3
localization, in dorsal hypothalamus. The immunoreactivity (ir) gradually increased in PD15
and PD20, in clusters of cells in the lateral, ventral and dorsomedial hypothalamus.
Furthermore, in PD20 animals, NTPDase3-ir was detected on short fibers in the posterior
hypothalamic area, while in PD30 the fibers appeared progressively longer and markedly
varicose. In adults, the strongest NTPDase3-ir was observed in collections of cells in
dorsomedial hypothalamic nucleus, dorsal and lateral hypothalamus and in several thalamic
areas, whereas the varicose fibers traversed entire diencephalon, particularly 
paraventricular
thalamic nucleus, ventromedial and dorsomedial hypothalamic nuclei, the arcuate nucleus and
the prefornical part of the lateral hypothalamus. The presumably ascending NTPDase3-ir
fibers were first observed in PD20; their density and the varicose appearance increased 
until
the adulthood. Prominent enhancement of NTPDase3-ir in the hypothalamus coincides with
age when animals acquire diurnal rhythms of sleeping and feeding, supporting the hypothesis
that this enzyme may be involved in regulation of homeostatic functions.
2021-03-09 02:27