{ "labelLang" : "hun", "responseDate" : "2024-03-29 03:08", "content" : { "otype" : "Citation", "mtid" : 24139930, "status" : "APPROVED", "published" : true, "comment" : "Abstract\n\nEctonucleotidases are membrane-bound or secreted proteins that hydrolyze extracellular \nnucleotides. Recently, we identified three ectonucleotidases that hydrolyze extracellular \nadenosine 5’-monophosphate (AMP) to adenosine in primary somatosensory neurons. Currently, \nit is unclear which ectonucleotidases hydrolyze ATP and ADP in these neurons. \nEctonucleoside triphosphate diphosphohydrolases (ENTPDs) comprise a class of enzymes that \ndephosphorylate extracellular ATP and ADP. Here, we found that ENTPD3 (also known as \nNTPDase3 or CD39L3) was located in nociceptive and non-nociceptive neurons of the dorsal \nroot ganglion (DRG), in the dorsal horn of the spinal cord, and in free nerve endings in \nthe skin. To determine if ENTPD3 contributes directly to ATP and ADP hydrolysis in these \ntissues, we generated and characterized an Entpd3 knockout mouse. This mouse lacks ENTPD3 \nprotein in all tissues examined, including the DRG, spinal cord, skin, and bladder. \nHowever, DRG and spinal cord tissues from Entpd3-/- mice showed no reduction in \nhistochemical staining when ATP, ADP, AMP, or UTP were used as substrates. Additionally, \nusing fast-scan cyclic voltammetry (FSCV), adenosine production was not impaired in the \ndorsal spinal cord of Entpd3-/- mice when the substrate ADP was applied. Further, Entpd3-\n/- mice did not differ in nociceptive behaviors when compared to wild-type mice, although \nEntpd3-/- mice showed a modest reduction in β-alanine-mediated itch. Taken together, our \ndata indicate that deletion of Entpd3 does not impair ATP or ADP hydrolysis in primary \nsomatosensory neurons or in dorsal spinal cord. Moreover, our data suggest there could be \nmultiple ectonucleotidases that act redundantly to hydrolyze nucleotides in these regions \nof the nervous system.", "unhandledTickets" : 0, "oldTimestamp" : "2015-05-07T14:36:03.000+0000", "deleted" : false, "oldId" : 14139930, "lastRefresh" : "2023-09-19T03:30:12.681+0000", "lastModified" : "2015-05-07T14:36:03.000+0000", "created" : "2014-08-26T08:14:23.000+0000", "adminApproved" : "2015-01-21T11:59:35.000+0000", "adminApprover" : { "otype" : "Admin", "mtid" : 10047806, "link" : "/api/admin/10047806", "label" : "Mudri Diána (SZIE ÁOTK könyvtáros, admin)", "familyName" : "Mudri", "givenName" : "Diána", "published" : true, "oldId" : 10047806, "snippet" : true }, "tempLockers" : [ { "otype" : "Admin", "mtid" : 10073918, "link" : "/api/admin/10073918", "label" : "Kovács Marcell (MTMT Közp., admin)", "familyName" : "Kovács", "givenName" : "Marcell", "published" : true, "snippet" : true } ], "tempLocked" : "2021-09-15T08:56:12.404+0000", "publication" : { "otype" : "JournalArticle", "mtid" : 1228647, "link" : "/api/publication/1228647", "label" : "Kiss David S et al. Ecto-nucleoside triphosphate diphosphohydrolase 3 in the ventral and lateral hypothalamic area of female rats: morphological characterization and functional implications. 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