Idézéskapcsolat

Eric McCoy et al. Deletion of ENTPD3 does not impair nucleotide hydrolysis in primary somatosensory neurons or spinal cord. (2014) F1000RESEARCH 2046-1402 2046-1402 3 p. 163

Közlemény:
S Kiss David et al. Ecto-nucleoside triphosphate diphosphohydrolase 3 in the ventral and lateral hypothalamic area of female rats: morphological characterization and functional implications. (2009) REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY 1477-7827 1477-7827 7 p. 31
Idéző közlemény:
Eric McCoy et al. Deletion of ENTPD3 does not impair nucleotide hydrolysis in primary somatosensory neurons or spinal cord. (2014) F1000RESEARCH 2046-1402 2046-1402 3 p. 163
Független: Igen
Megjegyzés: Abstract

Ectonucleotidases are membrane-bound or secreted proteins that hydrolyze extracellular 
nucleotides. Recently, we identified three ectonucleotidases that hydrolyze extracellular 
adenosine 5’-monophosphate (AMP) to adenosine in primary somatosensory neurons. Currently, 
it is unclear which ectonucleotidases hydrolyze ATP and ADP in these neurons. 
Ectonucleoside triphosphate diphosphohydrolases (ENTPDs) comprise a class of enzymes that 
dephosphorylate extracellular ATP and ADP. Here, we found that ENTPD3 (also known as 
NTPDase3 or CD39L3) was located in nociceptive and non-nociceptive neurons of the dorsal 
root ganglion (DRG), in the dorsal horn of the spinal cord, and in free nerve endings in 
the skin. To determine if ENTPD3 contributes directly to ATP and ADP hydrolysis in these 
tissues, we generated and characterized an Entpd3 knockout mouse. This mouse lacks ENTPD3 
protein in all tissues examined, including the DRG, spinal cord, skin, and bladder. 
However, DRG and spinal cord tissues from Entpd3-/- mice showed no reduction in 
histochemical staining when ATP, ADP, AMP, or UTP were used as substrates. Additionally, 
using fast-scan cyclic voltammetry (FSCV), adenosine production was not impaired in the 
dorsal spinal cord of Entpd3-/- mice when the substrate ADP was applied. Further, Entpd3-
/- mice did not differ in nociceptive behaviors when compared to wild-type mice, although 
Entpd3-/- mice showed a modest reduction in β-alanine-mediated itch. Taken together, our 
data indicate that deletion of Entpd3 does not impair ATP or ADP hydrolysis in primary 
somatosensory neurons or in dorsal spinal cord. Moreover, our data suggest there could be 
multiple ectonucleotidases that act redundantly to hydrolyze nucleotides in these regions 
of the nervous system.
2021-03-09 03:26