{ "labelLang" : "hun", "responseDate" : "2024-03-28 22:07", "paging" : { "last" : true, "first" : true, "totalPages" : 1, "totalElements" : 10, "size" : 10, "number" : 1, "numberOfElements" : 10, "sort" : [ { "direction" : "DESC", "property" : "publishedYear", "ascending" : false }, { "direction" : "ASC", "property" : "firstAuthor", "ascending" : true }, { "direction" : "ASC", "property" : "mtid", "ascending" : true } ] }, "content" : [ { "otype" : "JournalArticle", "mtid" : 32111413, "status" : "VALIDATED", "published" : true, "comment" : "Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, Greece \n Department of Microbiology, ELTE Eötvös Loránd University, Budapest, Hungary \n Cited By :4 \n Export Date: 8 December 2022 \n CODEN: AEMID", "unhandledTickets" : 0, "deleted" : false, "lastRefresh" : "2024-02-21T13:10:59.384+0000", "lastModified" : "2021-09-29T19:01:15.200+0000", "created" : "2021-07-23T13:11:44.255+0000", "creator" : { "otype" : "Admin", "mtid" : 10019782, "link" : "/api/admin/10019782", "label" : "Csámer Iván (ELTE TTK kari admin 5)", "familyName" : "Csámer", "givenName" : "Iván", "published" : true, "oldId" : 10019782, "snippet" : true }, "lastDuplumOK" : "2023-07-04T13:52:38.238+0000", "lastDuplumSearch" : "2023-07-04T13:52:38.238+0000", "validated" : "2021-09-29T19:01:15.209+0000", "validator" : { "otype" : "Admin", "mtid" : 10071503, "link" : "/api/admin/10071503", "label" : "Szabó Regina (ELTE TTK admin 5)", "familyName" : "Szabó", "givenName" : "Regina", "published" : true, "snippet" : true }, "core" : true, "citation" : true, "publicationPending" : false, "type" : { "otype" : "PublicationType", "mtid" : 24, "link" : "/api/publicationtype/24", "label" : "Folyóiratcikk", "code" : 24, "otypeName" : "JournalArticle", "listPosition" : 1, "published" : true, "oldId" : 24, "snippet" : true }, "subType" : { "otype" : "SubType", "mtid" : 10000059, "link" : "/api/subtype/10000059", "label" : "Szakcikk (Folyóiratcikk)", "name" : "Szakcikk", "nameEng" : "Article", "docType" : { "otype" : "PublicationType", "mtid" : 24, "link" : "/api/publicationtype/24", "label" : "Folyóiratcikk", "code" : 24, "otypeName" : "JournalArticle", "listPosition" : 1, "published" : true, "oldId" : 24, "snippet" : true }, "listPosition" : 101, "published" : true, "oldId" : 10000059, "snippet" : true }, "category" : { "otype" : "Category", "mtid" : 1, "link" : "/api/category/1", "label" : "Tudományos", "published" : true, "oldId" : 1, "snippet" : true }, "languages" : [ { "otype" : "Language", "mtid" : 10002, "link" : "/api/language/10002", "label" : "Angol", "name" : "Angol", "nameEng" : "English", "published" : true, "oldId" : 2, "snippet" : true } ], "firstAuthor" : "Garefalaki, Vasiliki", "authorships" : [ { "otype" : "PersonAuthorship", "mtid" : 96877205, "link" : "/api/authorship/96877205", "label" : "Garefalaki, Vasiliki", "listPosition" : 1, "share" : 0.125, "first" : true, "last" : false, "corresponding" : false, "familyName" : "Garefalaki", "givenName" : "Vasiliki", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 96877206, "link" : "/api/authorship/96877206", "label" : "Papavergi, Maria-Giusy", "listPosition" : 2, "share" : 0.125, "first" : false, "last" : false, "corresponding" : false, "familyName" : "Papavergi", "givenName" : "Maria-Giusy", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 96877207, "link" : "/api/authorship/96877207", "label" : "Savvidou, Olga", "listPosition" : 3, "share" : 0.125, "first" : false, "last" : false, "corresponding" : false, "familyName" : "Savvidou", "givenName" : "Olga", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 96877208, "link" : "/api/authorship/96877208", "label" : "Papanikolaou, Georgia", "listPosition" : 4, "share" : 0.125, "first" : false, "last" : false, "corresponding" : false, "familyName" : "Papanikolaou", "givenName" : "Georgia", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 96877209, "link" : "/api/authorship/96877209", "label" : "Felföldi, Tamás [Felföldi, Tamás (mikrobiológia), szerző] Mikrobiológiai Tanszék (ELTE / TTK / Bio_I)", "listPosition" : 5, "share" : 0.125, "first" : false, "last" : false, "corresponding" : false, "author" : { "otype" : "Author", "mtid" : 10020429, "link" : "/api/author/10020429", "label" : "Felföldi Tamás (mikrobiológia)", "familyName" : "Felföldi", "givenName" : "Tamás", "published" : true, "oldId" : 10020429, "snippet" : true }, "familyName" : "Felföldi", "givenName" : "Tamás", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 96877210, "link" : "/api/authorship/96877210", "label" : "Márialigeti, Károly [Márialigeti, Károly (Mikrobiológia), szerző] Mikrobiológiai Tanszék (ELTE / TTK / Bio_I)", "listPosition" : 6, "share" : 0.125, "first" : false, "last" : false, "corresponding" : false, "author" : { "otype" : "Author", "mtid" : 10005182, "link" : "/api/author/10005182", "label" : "Márialigeti Károly (Mikrobiológia)", "familyName" : "Márialigeti", "givenName" : "Károly", "published" : true, "oldId" : 10005182, "snippet" : true }, "familyName" : "Márialigeti", "givenName" : "Károly", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 96877211, "link" : "/api/authorship/96877211", "label" : "Fakis, Giannoulis", "listPosition" : 7, "share" : 0.125, "first" : false, "last" : false, "corresponding" : false, "familyName" : "Fakis", "givenName" : "Giannoulis", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true }, { "otype" : "PersonAuthorship", "mtid" : 96877212, "link" : "/api/authorship/96877212", "label" : "Boukouvala, Sotiria", "listPosition" : 8, "share" : 0.125, "first" : false, "last" : true, "corresponding" : false, "familyName" : "Boukouvala", "givenName" : "Sotiria", "authorTyped" : true, "editorTyped" : false, "otherTyped" : false, "type" : { "otype" : "AuthorshipType", "mtid" : 1, "link" : "/api/authorshiptype/1", "label" : "Szerző", "code" : 0, "published" : true, "oldId" : 0, "snippet" : true }, "published" : false, "snippet" : true } ], "title" : "Comparative Investigation of Fifteen Xenobiotic Metabolizing N-Acetyltransferase (NAT) Homologs from Bacteria.", "identifiers" : [ { "otype" : "PublicationIdentifier", "mtid" : 19037155, "link" : "/api/publicationidentifier/19037155", "label" : "DOI: 10.1128/AEM.00819-21", "source" : { "otype" : "PlainSource", "mtid" : 6, "link" : "/api/publicationsource/6", "label" : "DOI", "type" : { "otype" : "PublicationSourceType", "mtid" : 10001, "link" : "/api/publicationsourcetype/10001", "label" : "DOI", "mayHaveOa" : true, "published" : true, "snippet" : true }, "name" : "DOI", "nameEng" : "DOI", "linkPattern" : "https://doi.org/@@@", "publiclyVisible" : true, "published" : true, "oldId" : 6, "snippet" : true }, "validState" : "IDENTICAL", "idValue" : "10.1128/AEM.00819-21", "realUrl" : "https://doi.org/10.1128/AEM.00819-21", "published" : false, "snippet" : true }, { "otype" : "PublicationIdentifier", "mtid" : 19440419, "link" : "/api/publicationidentifier/19440419", "label" : "WoS: 000697455200005", "source" : { "otype" : "PlainSource", "mtid" : 1, "link" : "/api/publicationsource/1", "label" : "WoS", "type" : { "otype" : "PublicationSourceType", "mtid" : 10003, "link" : "/api/publicationsourcetype/10003", "label" : "Indexelő adatbázis", "mayHaveOa" : false, "published" : true, "snippet" : true }, "name" : "WoS", "nameEng" : "WoS", "linkPattern" : "https://www.webofscience.com/wos/woscc/full-record/@@@", "publiclyVisible" : true, "published" : true, "oldId" : 1, "snippet" : true }, "validState" : "IDENTICAL", "idValue" : "000697455200005", "realUrl" : "https://www.webofscience.com/wos/woscc/full-record/000697455200005", "published" : false, "snippet" : true }, { "otype" : "PublicationIdentifier", "mtid" : 22210388, "link" : "/api/publicationidentifier/22210388", "label" : "Scopus: 85116441763", "source" : { "otype" : "PlainSource", "mtid" : 3, "link" : "/api/publicationsource/3", "label" : "Scopus", "type" : { "otype" : "PublicationSourceType", "mtid" : 10003, "link" : "/api/publicationsourcetype/10003", "label" : "Indexelő adatbázis", "mayHaveOa" : false, "published" : true, "snippet" : true }, "name" : "Scopus", "linkPattern" : "http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-@@@", "publiclyVisible" : true, "published" : true, "oldId" : 3, "snippet" : true }, "validState" : "IDENTICAL", "idValue" : "85116441763", "realUrl" : "http://www.scopus.com/record/display.url?origin=inward&eid=2-s2.0-85116441763", "published" : false, "snippet" : true }, { "otype" : "PublicationIdentifier", "mtid" : 19037154, "link" : "/api/publicationidentifier/19037154", "label" : "PubMed: 34288706", "source" : { "otype" : "PlainSource", "mtid" : 17, "link" : "/api/publicationsource/17", "label" : "PubMed", "type" : { "otype" : "PublicationSourceType", "mtid" : 10003, "link" : "/api/publicationsourcetype/10003", "label" : "Indexelő adatbázis", "mayHaveOa" : false, "published" : true, "snippet" : true }, "name" : "PubMed", "nameEng" : "PubMed", "linkPattern" : "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=@@@&dopt=Abstract", "publiclyVisible" : true, "published" : true, "oldId" : 17, "snippet" : true }, "validState" : "IDENTICAL", "idValue" : "34288706", "realUrl" : "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=34288706&dopt=Abstract", "published" : false, "snippet" : true } ], "journal" : { "otype" : "Journal", "mtid" : 451, "link" : "/api/journal/451", "label" : "APPLIED AND ENVIRONMENTAL MICROBIOLOGY 0099-2240 1098-5336", "pIssn" : "0099-2240", "eIssn" : "1098-5336", "reviewType" : "REVIEWED", "noIF" : false, "sciIndexed" : true, "scopusIndexed" : true, "lang" : "FOREIGN", "hungarian" : false, "published" : true, "oldId" : 451, "snippet" : true }, "volume" : "87", "issue" : "19", "internalId" : "e00819-21", "firstPageOrInternalIdForSort" : "e00819-21", "publishedYear" : 2021, "abstractText" : "Arylamines constitute a large group of industrial chemicals detoxified by certain bacteria through conjugation reactions catalyzed by N-acetyltransferase (NAT) enzymes. NAT homologs, mostly from pathogenic bacteria, have been the subject of individual studies that do not facilitate direct comparisons. By implementing a practicable pipeline, we present comparative investigation of fifteen NAT homologs from ten bacteria, mainly bacilli, streptomycetes, and one alphaproteobacterium. The new homologs were characterized for their sequence, phylogeny, predicted structural features, substrate specificity, thermal stability, and interaction with components of the enzymatic reaction. Bacillus NATs demonstrated the characteristics of xenobiotic metabolizing N-acetyltransferases, with the majority of homologs generating high activities. Non-pathogenic bacilli are thus proposed as suitable mediators of arylamine bioremediation. Of the Streptomyces homologs, the NAT2 isoenzyme of S. venezuelae efficiently transformed highly toxic arylamines, while the remaining homologs were inactive or generated low activities suggesting that xenobiotic metabolism may not be their primary role. The functional divergence of Streptomyces NATs was consistent with their observed sequence, phylogenetic, and structural variability. These and previous findings support classification of microbial NATs into three groups. The first includes xenobiotic metabolizing enzymes with dual acetyl-/propionyl-CoA selectivity. Homologs of the second group are more rarely encountered, acting as malonyltransferases mediating specialized ecological interactions. Homologs of the third group effectively lack acyltransferase activity and their study may represent an interesting research area. Comparative NAT enzyme screens from a broad microbial spectrum may guide rational selection of homologs likely to share similar biological functions, allowing their combined investigation and use in biotechnological applications. IMPORTANCE Arylamines are encountered as industrial chemicals or byproducts of agrochemicals that may constitute highly toxic contaminants of soils and groundwaters. Although such chemicals may be recalcitrant to biotransformation, they can be enzymatically converted into less toxic forms by some bacteria. Therefore, exploitation of the arylamine detoxification capabilities of microorganisms is investigated as an effective approach for bioremediation. Among microbial biotransformations of arylamines, enzymatic conjugation reactions have been reported, including NAT-mediated N-acetylation. Comparative investigations of NAT enzymes across a range of microorganisms can be laborious and expensive, so here we present a streamlined methodology for implementing such work. We compare fifteen NAT homologs from non-pathogenic, free-living bacteria of potential biotechnological utility, mainly Terrabacteria known for their rich secondary and xenobiotic metabolism. The analysis allowed insights into the evolutionary and functional divergence of bacterial NAT homologs, combined with assessment of their fundamental structural and enzymatic differences and similarities.", "digital" : null, "printed" : null, "sourceYear" : 2021, "foreignEdition" : true, "foreignLanguage" : true, "fullPublication" : true, "conferencePublication" : false, "nationalOrigin" : null, "missingAuthor" : false, "oaType" : "NONE", "oaCheckDate" : "2024-02-21", "oaFree" : false, "citationCount" : 4, "citationCountUnpublished" : 0, "citationCountWoOther" : 4, "independentCitCountWoOther" : 2, "nationalOriginCitationCount" : 0, "foreignEditionCitationCount" : 4, "doiCitationCount" : 4, "wosCitationCount" : 4, "scopusCitationCount" : 4, "wosScopusCitationCount" : 4, "wosScopusCitationCountWoOther" : 4, "wosScopusIndependentCitationCount" : 2, "wosScopusIndependentCitationCountWoOther" : 2, "independentCitationCount" : 2, "selfCitationCount" : 2, "unhandledCitationCount" : 0, "citingPubCount" : 4, "independentCitingPubCount" : 2, "citingPubCountWoOther" : 4, "independentCitingPubCountWoOther" : 2, "unhandledCitingPubCount" : 0, "citedPubCount" : 1, "citedCount" : 1, "ratings" : [ { "otype" : "SjrRating", "mtid" : 11187308, "link" : "/api/sjrrating/11187308", "label" : "sjr:D1 (2021) Scopus - Food Science APPLIED AND ENVIRONMENTAL MICROBIOLOGY 0099-2240 1098-5336", "listPos" : 27, "rankValue" : 0.1, "type" : "journal", "ratingType" : { "otype" : "RatingType", "mtid" : 10002, "link" : "/api/ratingtype/10002", "label" : "sjr", "code" : "sjr", "published" : true, "snippet" : true }, "subject" : { "otype" : "ClassificationExternal", "mtid" : 1106, "link" : "/api/classificationexternal/1106", "label" : "Scopus - Food Science", "published" : true, "oldId" : 1106, "snippet" : true }, "ranking" : "D1", "calculation" : "DIRECT", "published" : true, "snippet" : true } ], "ratingsForSort" : "D1", "hasCitationDuplums" : false, "inSelectedPubs" : "10020429,10005182", "userChangeableUntil" : "2021-09-28T19:01:15.017+0000", "directInstitutesForSort" : "Mikrobiológiai Tanszék (ELTE / TTK / Bio_I)", "ownerAuthorCount" : 2, "ownerInstituteCount" : 8, "directInstituteCount" : 1, "authorCount" : 8, "contributorCount" : 0, "hasQualityFactor" : true, "link" : "/api/publication/32111413", "label" : "Garefalaki Vasiliki et al. Comparative Investigation of Fifteen Xenobiotic Metabolizing N-Acetyltransferase (NAT) Homologs from Bacteria.. (2021) APPLIED AND ENVIRONMENTAL MICROBIOLOGY 0099-2240 1098-5336 87 19", "template" : "